A5072629352- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Cardiovascular Function and Risk Factors
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Metabolism, Diabetes, and Cancer
- Cardiac Ischemia and Reperfusion
- Telomeres, Telomerase, and Senescence
- Advanced MIMO Systems Optimization
- Energy Harvesting in Wireless Networks
- Autophagy in Disease and Therapy
- Cancer Genomics and Diagnostics
- Inflammasome and immune disorders
- Stress Responses and Cortisol
- Advanced Wireless Network Optimization
- Pulmonary Hypertension Research and Treatments
- Endoplasmic Reticulum Stress and Disease
- Advanced biosensing and bioanalysis techniques
- Cardiac Valve Diseases and Treatments
University of Pittsburgh
2016-2025
University of Pittsburgh Medical Center
2018-2024
University of Pennsylvania
2011-2023
ORCID
2021
Pittsburg State University
2016-2019
United Mitochondrial Disease Foundation
2019
Aalto University
2015
Sheba Medical Center
2014
Tel Aviv University
2014
Rice University
2007-2013
The BRCA1/2 proteins are involved in regulation of cellular proliferation by DNA damage repair via homologous recombination. Therefore, mutation carriers with pancreatic cancer may have distinct biologic outcomes. Patients BRCA1/2-associated ductal adenocarcinoma (PDAC) diagnosed between January 1994 and December 2012 were identified from databases at three participating institutions. Clinical data collected. Disease-free survival overall (OS) analysed. Overall, 71 patients PDAC BRCA1...
Packaging DNA into condensed structures is integral to the transmission of genomes. The mammalian mitochondrial genome (mtDNA) a high copy, maternally inherited in which mutations cause variety multisystem disorders. In all eukaryotic cells, multiple mtDNAs are packaged with protein spheroid bodies called nucleoids, fundamental units mtDNA segregation. mechanism nucleoid formation, however, remains unknown. Here, we show that transcription factor TFAM, an abundant and highly conserved High...
The mechanisms that ensure the removal of damaged mitochondrial proteins and lipids are critical for health cell, errors in these pathways implicated numerous degenerative diseases. We recently uncovered a new pathway selective mediated by derived vesicular carriers (MDVs) transit to lysosome. However, it was not determined whether vesicles were selectively enriched oxidized, or proteins, extent which complexes electron transport chain mtDNA-containing nucloids may have been incorporated. In...
Abstract Patients with primary mitochondrial oxidative phosphorylation (OxPhos) defects present fatigue and multi-system disorders, are often lean, die prematurely, but the mechanistic basis for this clinical picture remains unclear. By integrating data from 17 cohorts of patients diseases ( n = 690) we find evidence that these disorders increase resting energy expenditure, a state termed hypermetabolism . We examine phenomenon longitudinally in patient-derived fibroblasts multiple donors....
Mitochondrial DNA (mtDNA) is essential for cells to maintain respiratory competency and inherited as a protein-DNA complex called the nucleoid. We have identified 22 mtDNA-associated proteins in yeast, among which mitochondrial aconitase (Aco1p). show that this Krebs-cycle enzyme mtDNA maintenance independent of its catalytic activity. Regulation ACO1 expression by HAP retrograde metabolic signaling pathways directly affects maintenance. When constitutively expressed, Aco1p can replace...
The letter presents experimental results for a proximity-coupled microstrip patch antenna capable of 13% bandwidth. impedance match (VSWR ≤ 2), copolarised radiation patterns and crosspolarised were measured over this bandwidth to confirm operation. construction is quite simple, consisting feedline on substrate rectangular covering superstrate; small open-circuit tuning stub connected in shunt with the feed line.
Three methods for the measurement of antenna efficiency are evaluated: (1) Wheeler cap method, (2) radiometric and (3) directivity/gain method. Each these was used to measure four different printed antennas (three microstrip patches with various feeds, an eight-element series-fed array). These experimental results which were obtained discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Spectrum sharing is a novel opportunistic strategy to improve spectral efficiency of wireless networks. Much the research quantify such gain done under premise that spectrum being used inefficiently by primary network. Our main result even in spectrally efficient network, device users can exploit network topology render gains additional throughput. The focus will be on providing ad-hoc multihop access for users, are transparent cellular while network's resources.
The segregating unit of mtDNA is a protein-DNA complex called the nucleoid. In an effort to understand how nucleoid proteins contribute organization and inheritance, we have developed in organello formaldehyde crosslinking procedure identify associated with mtDNA. Using highly purified mitochondria, observed time-dependent protein as determined by sedimentation through isopycnic cesium chloride gradients. We detected approximately 20 crosslinked identified 11, mostly mass spectrometry. Among...
In an attempt to utilize spectrum resources more efficiently, protocols sharing licensed with unlicensed users are receiving increased attention. From the perspective of cellular networks, underutilization makes spatial reuse a feasible complement existing standards. Interference management is major component in designing these schemes as it critical that maintain their expected quality service. We develop distributed dynamic protocol which ad-hoc device-to-device opportunistically access...
Calcium signaling stimulates the accumulation of mitochondrial calcium uniporter to regulate metabolism.
Mitochondrial DNA (mtDNA) deletions cause disease and accumulate during aging, yet our understanding of the molecular mechanisms underlying their formation remains rudimentary. Guanine-quadruplex (GQ) structures are associated with nuclear instability in cancer; recent evidence indicates they can also form mitochondrial nucleic acids, suggesting that these non-B could be mtDNA deletions. Currently, multiple types GQ sequences association human stability unknown. Here, we show an between...
Abstract Single-stranded DNA or RNA sequences rich in guanine (G) can adopt non-canonical structures known as G-quadruplexes (G4). Mitochondrial (mtDNA) that are predicted to form G4 enriched on the heavy-strand and have been associated with formation of deletion breakpoints. Increasing evidence supports ability mtDNA cancer cells; however, functional roles regulating mitochondrial nucleic acid homeostasis non-cancerous cells remain unclear. Here, we demonstrate by live cell imaging...
We have previously shown that endoplasmic reticulum stress (ER stress) represses the PTEN inducible kinase 1 (PINK1) in lung type II alveolar epithelial cells (AECII) reducing mitophagy and increasing susceptibility to fibrosis. Although increased circulating mitochondrial DNA (mtDNA) has been reported chronic diseases, contribution of modulation DAMP release activation profibrotic responses is unknown. In this study, we show ER PINK1 deficiency AECII led with significant oxidation damage...
Abstract The dynamin-like GTPases Mitofusin 1 and 2 (Mfn1 Mfn2) are essential for mitochondrial function, which has been principally attributed to their regulation of fission/fusion dynamics. Here, we report that Mfn1 critical glucose-stimulated insulin secretion (GSIS) primarily through control DNA (mtDNA) content. Whereas Mfn2 individually were dispensable glucose homeostasis, combined Mfn1/2 deletion in β-cells reduced mtDNA content, impaired morphology networking, decreased respiratory...