A5089362139- Identification and Quantification in Food
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Environmental DNA in Biodiversity Studies
- Forensic and Genetic Research
- Genetic and phenotypic traits in livestock
- Wildlife Ecology and Conservation
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Viral gastroenteritis research and epidemiology
- Human-Animal Interaction Studies
- Genetic Mapping and Diversity in Plants and Animals
- Molecular Biology Techniques and Applications
- Ichthyology and Marine Biology
- Rabies epidemiology and control
- Animal Virus Infections Studies
- CRISPR and Genetic Engineering
- Metabolism and Genetic Disorders
- Fish Biology and Ecology Studies
- SARS-CoV-2 detection and testing
- Animal Diversity and Health Studies
- Cancer therapeutics and mechanisms
- Immunotherapy and Immune Responses
- Species Distribution and Climate Change
Lund University
2021-2024
Centro de Genética Clínica
2021-2023
University of Trás-os-Montes and Alto Douro
2023
Universidade do Porto
2012-2022
University of Coimbra
2012-2022
University of Minho
2022
Polytechnic Institute of Cávado and Ave
2022
Poznań University of Technology
2022
University of Maribor
2022
Cornell University
2022
Sheep retroviruses can be used to map the selective preferences of early farmers and trace livestock movements across Europe.
Endogenous retroviruses (ERVs) are remnants of ancient retroviral infections the host germline transmitted vertically from generation to generation. It is hypothesized that some ERVs used by as restriction factors block infection pathogenic retroviruses. Indeed, efficiently interfere with replication related exogenous However, data suggesting these mechanisms have influenced coevolution endogenous and/or and their hosts been more difficult obtain. Sheep an interesting model system study...
Mitochondrial DNA (mtDNA) deletions cause disease and accumulate during aging, yet our understanding of the molecular mechanisms underlying their formation remains rudimentary. Guanine-quadruplex (GQ) structures are associated with nuclear instability in cancer; recent evidence indicates they can also form mitochondrial nucleic acids, suggesting that these non-B could be mtDNA deletions. Currently, multiple types GQ sequences association human stability unknown. Here, we show an between...
Nature is the major reservoir of biologically active molecules. The urgent need finding novel molecules for pharmaceutical application prompting research underexplored environments, such as marine ecosystems. Here, we investigated cultivable actinobacteria associated with macroalgae Laminaria ochroleuca and assessed their potential to produce compounds antimicrobial or anticancer activities. A specimen L. was collected in a rocky shore northern Portugal, fragments tissues from different...
Highly adaptable and versatile populations of domestic sheep, the result millennia intense husbandry, are found in almost every corner world. Here we describe a genetic survey sheep from western fringe its European distribution. We studied mitochondrial DNA control region sequences 161 individuals belonging to 7 Portuguese breeds. Our study revealed high level diversity, with an average breed haplotype diversity 0.983, substantially above that observed central breeds, as well presence...
Valuable insights into the history of human populations have been obtained by studying genetic composition their domesticated species. Here we address some long-standing questions about origin and subsequent movements goat pastoralism in Northern Africa. We present first study combining results from mitochondrial DNA (mtDNA) Y chromosome loci for characterization a domestic population. Our analyses indicate remarkably high diversity maternal paternal lineages sample indigenous goats...
Mitochondrial DNA (mtDNA) mutations are a common cause of primary mitochondrial disorders, and have also been implicated in broad collection conditions, including aging, neurodegeneration, cancer. Prevalent among these pathogenic variants mtDNA deletions, which show strong bias for the loss sequence major arc between, but not including, heavy light strand origins replication. Because individual deletions can accumulate focally, occur with multiple mixed breakpoints, presence normal...
The quest for a universal and efficient method of identifying species has been longstanding challenge in biology. Here, we show that accurate identification all domains life can be accomplished by multiplex analysis variable-length sequences containing multiple insertion/deletion variants. new method, called SPInDel, is able to discriminate 93.3% eukaryotic from 18 taxonomic groups. We also demonstrate the prokaryotic viral with numeric profiles fragment lengths generally straightforward. A...
Mitochondrial DNA (mtDNA) deletions are a primary cause of mitochondrial disease and believed to contribute the aging process various neurodegenerative diseases. Despite strong observational experimental evidence, molecular basis deletion remains obscure. In this study, we test hypothesis that mtDNA vulnerability breakage resides in formation non-B conformations, namely hairpin, cruciform cloverleaf-like elements. Using largest database human built thus far (753 different cases), show...
Mitochondrial DNA (mtDNA) rearrangements are key events in the development of many diseases. Investigations mtDNA regions affected by (i.e. breakpoints) can lead to important discoveries about rearrangement mechanisms and offer clues causes mitochondrial Here, we present breakpoints database (MitoBreak; http://mitobreak.portugene.com), a free, web-accessible comprehensive list from three classes somatic rearrangements: circular deleted (deletions), partially duplicated (duplications) linear...
The pinewood nematode, Bursaphelenchus xylophilus, is one of the greatest threats to coniferous forests worldwide, causing severe ecological damage and economic loss. biology B. xylophilus similar that its closest relative, mucronatus, as both species share food resources insect vectors, have very morphological characteristics, although little pathogenicity conifers has been associated with mucronatus. Using nuclear mitochondrial DNA markers, we show mucronatus form distinct phylogenetic...
A novel method is presented here as an analytical tool for food control and authentication of dairy products manufactured from the milk cow, sheep, goat, buffalo. The based on multiplex polymerase chain reaction (PCR) species-specific mitochondrial DNA (mtDNA) targets followed by fragment size analysis capillary electrophoresis. includes (a) simultaneous detection four species, (b) internal extraction PCR, (c) mtDNA a target (d) amplicons <200 bp, (e) flexibility in electrophoresis method....
Here we present a mitogenomic perspective on the evolution of sharks and rays, being first glance complete mitochondrial history such an old diversified group vertebrates. The Elasmobranchii is diverse subclass Chondrichthyes, or cartilaginous fish, with about 1200 species ocean- freshwater-dwelling fishes spread all over world's seas, including some ocean's largest fishes. dates back 400 million years near Devonian–Silurian boundary, nowadays represented by several derivative lineages,...
Abstract Goat is believed to be the first true livestock domesticated and, apart from its historical importance, keeps playing an essential economic role in very diverse human societies. We have analysed female gene pool of all Portuguese autochthonous breeds (Bravia, Serrana, Charnequeira, Serpentina and Algarvia) through mtDNA HVI sequencing 288 unrelated animals sampled throughout country. All proved extremely (average haplotype diversity 0.977), contrast with peripheral geographic...
A combined effect of functional constraints and random mutational events is responsible for the sequence evolution human mitochondrial DNA (mtDNA) control region. Most studies targeting this noncoding segment usually focus on its primary information disregarding other informative levels such as secondary or tertiary conformations. In work, we most recent developments in folding calculations with a phylogenetic comparative approach order to investigate formation intrastrand structures mtDNA...
Abstract A single multiplex PCR assay capable of simultaneously amplifying nine canine‐specific autosomal STR markers (FH3210, FH3241, FH2004, FH2658, FH4012, REN214L11, FH2010, FH2361 and the newly described C38) was developed for individual identification parentage testing in domestic dogs. In order to increase genotyping efficiency, amplicon sizes were optimized a 90–350 bp range, with fluorescently labelled primers use Applied Biosystems, Inc., platforms. The performance this new system...
Natural compounds have had increasing applications in the biotechnological sector, with a large fraction of these substances being channeled to pharmaceutical industry due their important pharmacological properties. The discovery new bioactive molecules novel mechanisms action constitutes promising solution for design alternative therapeutic solutions. Actinobacteria are group morphologically and physiologically diverse bacteria well known production biotechnologically relevant compounds....
Deletions in the 16.6 kb mitochondrial genome have been implicated numerous disorders that often display muscular and/or neurological symptoms due to high-energy demands of these tissues. We describe a catalogue 4489 putative DNA (mtDNA) deletions, including their frequency and relative read rate, using combinatorial approach mitochondria-targeted PCR, next-generation sequencing, bioinformatics, post-hoc filtering, annotation, validation steps. Our bioinformatics pipeline uses MapSplice, an...