A5029645183- Vibrio bacteria research studies
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Aquaculture disease management and microbiota
- Salmonella and Campylobacter epidemiology
- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Molecular Communication and Nanonetworks
- Genetics, Aging, and Longevity in Model Organisms
- Microbial Inactivation Methods
- Bacillus and Francisella bacterial research
- Aquatic Ecosystems and Biodiversity
- thermodynamics and calorimetric analyses
- Child Nutrition and Water Access
Tufts University
2017-2020
Georgia Institute of Technology
2014-2017
Institute of Bioinformatics
2008
Savitribai Phule Pune University
2008
The facultative pathogen Vibrio cholerae transitions between its human host and aquatic reservoirs where it colonizes chitinous surfaces. Growth on chitin induces expression of utilization genes, genes involved in DNA uptake by natural transformation, a type VI secretion system that allows contact-dependent killing neighboring bacteria. We have previously shown the transcription factor CytR, thought to primarily regulate pyrimidine nucleoside scavenging response, is required for competence...
ABSTRACT Horizontal gene transfer (HGT) can have profound effects on bacterial evolution by allowing individuals to rapidly acquire adaptive traits that shape their strategies for competition. One strategy intermicrobial antagonism often used Proteobacteria is the genetically encoded contact-dependent type VI secretion system (T6SS), a weapon kill heteroclonal neighbors direct injection of toxic effectors. Here, we experimentally demonstrate Vibrio cholerae new T6SS effector genes via...
Many bacteria use quorum sensing (QS) to regulate virulence factor production in response changes population density. QS is mediated through the production, secretion, and detection of signaling molecules called autoinducers (AIs) modulate population-wide behavioral changes. Four histidine kinases, LuxPQ, CqsS, CqsR VpsS, have been identified Vibrio cholerae as receptors activate gene expression at low cell Detection AIs by these leads repression high The redundancy among puzzling since any...
Like many bacteria, Vibrio cholerae deploys a harpoon-like type VI secretion system (T6SS) to compete against other microbes in environmental and host settings. The T6SS punctures adjacent cells delivers toxic effector proteins that are harmless bacteria carrying cognate immunity factors. Only four effector/immunity pairs encoded on one large three auxiliary gene clusters have been characterized from largely clonal, patient-derived strains of V. cholerae.We sequence two dozen strain genomes...
Abstract Many bacteria can become naturally competent to take up extracellular DNA across their outer and inner membranes by a dedicated competence apparatus. Whereas some studies show that the delivered cytoplasm may be used for genome repair or nutrition, it also recombined onto chromosome homologous recombination: process called natural transformation. Along with conjugation transduction, transformation represents mechanism horizontal transfer of genetic material, e.g., antibiotic...
The human pathogen Vibrio cholerae employs several adaptive mechanisms for environmental persistence, including natural transformation and type VI secretion, creating a reservoir the spread of disease. Here, we report whole-genome sequences 26 diverse V. isolates, significantly increasing sequence diversity publicly available genomes.
ABSTRACT The pathogen that causes cholera, Vibrio cholerae , uses the cell-cell communication process known as quorum sensing (QS) to regulate virulence factor production and biofilm formation in response changes population density complexity. QS is mediated through detection of extracellular chemical signals called autoinducers. Four histidine kinases, LuxPQ, CqsS, CqsR VpsS, have been identified receptors activate key regulator LuxO at low cell density. At high density, autoinducers by...
Genetically engineered bacteria can be used for a wide range of applications, from monitoring environmental toxins to studying complex communication networks in the human digestive system. Although great strides have been made single strains well-controlled microfluidic environments, there remains need tools reliably control and measure between multiple discrete bacterial populations. Stable long-term experiments (e.g., days) with controlled population sizes regulated input concentration)...
Abstract Aging is one of the most intriguing processes biology and despite decades research, many aspects aging are poorly understood. known to occur in bacteria yeast that divide with morphological asymmetry. Morphologically symmetrically dividing such as Escherichia coli were assumed not age until they shown functional asymmetry leading death some cells even exponentially growing cultures. In asymmetrically E. newly synthesized components presumed occupy pole so after division daughter...
Abstract Horizontal gene transfer can have profound effects on bacterial evolution by allowing individuals to rapidly acquire adaptive traits that shape their strategies for competition. One strategy intermicrobial antagonism often used Proteobacteria is the genetically-encoded contact-dependent Type VI secretion system (T6SS); a weapon kill heteroclonal neighbors direct injection of toxic effectors. Here, we experimentally demonstrate Vibrio cholerae new T6SS effector genes via horizontal...