A5091182112- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- interferon and immune responses
- Sex and Gender in Healthcare
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Hearing, Cochlea, Tinnitus, Genetics
- Research on Leishmaniasis Studies
- Respiratory viral infections research
- Transgenic Plants and Applications
- Immune responses and vaccinations
- Cancer Genomics and Diagnostics
- Immune Response and Inflammation
- Mosquito-borne diseases and control
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Ear and Head Tumors
- vaccines and immunoinformatics approaches
- Glioma Diagnosis and Treatment
- CAR-T cell therapy research
- Toxin Mechanisms and Immunotoxins
University of Manitoba
2008-2024
Research Manitoba
2019-2024
University of California, San Diego
2013-2017
The vaccinia virus double-stranded RNA binding protein E3 has been demonstrated to inhibit the expression of cytokines, including beta interferon (IFN-beta) and tumor necrosis factor alpha (TNF-alpha). However, few details regarding molecular mechanisms this inhibition have described. Using real-time PCR arrays, we found that suppressed induction a diverse array cytokines representing members IFN, interleukin (IL), TNF, transforming growth cytokine families. We discovered factor(s)...
Cellular tropism of vaccinia virus (VACV) is regulated by host range genes, including K1L, C7L, and E3L. While E3L known to support viral replication antagonizing interferon (IFN) effectors, PKR, the exact functions K1L C7L are unclear. Here, we show that can also inhibit antiviral effectors induced type I IFN. In human Huh7 MCF-7 cells, a VACV mutant lacking both (vK1L-C7L-) replicated as efficiently wild-type (WT) VACV, even in presence However, pretreating cells with IFN, while having...
Abstract Memory T cells are critical for secondary immunity against pathogens, yet their persistence in the absence of antigen remains unclear. While memory CD8 + known to persist independently cognate antigen, durability CD4 controversial. Recovery from cutaneous leishmaniasis confers lifelong immunity, largely mediated by cells, but necessity persistent parasites sustaining this has not been empirically confirmed. We investigated mice infected with a dihydrofolate reductase-thymidylate...
ABSTRACT Poxviruses are important human and animal pathogens that have evolved elaborate strategies for antagonizing host innate adaptive immunity. The E3 protein of vaccinia virus, the prototypic member orthopoxviruses, functions as an inhibitor immune signaling is essential virus replication in vivo many cell culture systems. However, function orthologues expressed by poxviruses other genera with different specificity remains largely unknown. In present study, we characterized from...
During an immune response against a microbial pathogen, activated naive T lymphocytes give rise to effector cells that provide acute host defense and memory long-lived immunity. It has been shown can undergo asymmetric division, enabling the daughter inherit unequal amounts of fate-determining proteins thereby acquire distinct fates from their inception. In this study, we show absence atypical protein kinase C (PKC) isoforms, PKCζ PKCλ/ι, disrupts CD8(+) lymphocyte division. These...
Abstract OTX2 is a potent oncogene that promotes tumor growth in Group 3 medulloblastoma. However, the mechanisms by which represses neural differentiation are not well characterized. Here, we perform extensive multiomic analyses to identify an regulatory network controls medulloblastoma cell fate. silencing modulates repressive chromatin landscape, decreases levels of PRC2 complex genes and increases expression neurodevelopmental transcription factors including PAX3 PAX6 . Expression...
Abstract Background Cisplatin-induced ototoxicity (CIO), characterized by irreversible and progressive bilateral hearing loss, is a prevalent adverse effect of cisplatin chemotherapy. Alongside clinical risk factors, genetic variants contribute to CIO genome-wide association studies (GWAS) have highlighted the polygenicity this drug reaction. Polygenic scores (PGS), which integrate information from multiple across genome, offer promising tool for identification individuals who are at higher...
<title>Abstract</title> CD8+ T cell diversity is essential to control infections and chronic antigen stimulation. In acute-resolving infection, effector cells mediate acute responses memory provide long-lived protection against future exposures. infection cancer, an altered state called exhaustion occurs. Exhausted are molecularly functionally distinct from cells. Differences in immune exist between biological sexes, however, how sex influences the timing transcriptional programs of during...
Abstract Although it is well established that heterogeneity of lymphocyte fate an essential feature adaptive immune responses, how and when these divergent cellular fates are specified remains unknown. It has been previously shown a T responding to microbial infection can undergo asymmetric division yield two daughter cells differentially fated from inception. Such model suggests the progeny arising each might exhibit distinct patterns gene expression. Because strategies analyzing bulk cell...