A5086818620- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Glioma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Bacterial Genetics and Biotechnology
- Internet Traffic Analysis and Secure E-voting
- Immune cells in cancer
- interferon and immune responses
- Connexins and lens biology
- Network Security and Intrusion Detection
- Cell Adhesion Molecules Research
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer-related gene regulation
- Animal Virus Infections Studies
- Software-Defined Networks and 5G
- Retinal Diseases and Treatments
- RNA modifications and cancer
- Pancreatic function and diabetes
- Chromosomal and Genetic Variations
- Cellular transport and secretion
University of California, San Diego
2017-2024
Chongqing University of Posts and Telecommunications
2024
Wuhan University
2014-2015
Single-cell sequencing analyses reveal changes in B and T cell transcriptional states the context of ulcerative colitis.
Heterogeneity of small intestine intraepithelial CD8 + T cells suggests functionally distinct subsets RM and their precursors.
Homologous recombination deficiency (HRD) is prevalent in cancer, sensitizing tumor cells to poly (ADP-ribose) polymerase (PARP) inhibition. However, the impact of HRD and related therapies on microenvironment (TME) remains elusive. Our study generates single-cell gene expression T cell receptor profiles, along with validatory multimodal datasets from >100 high-grade serous ovarian cancer (HGSOC) samples, primarily a phase II clinical trial (NCT04507841). Neoadjuvant monotherapy PARP...
During an immune response to microbial infection, CD8+ T cells give rise short-lived effector and memory that provide sustained protection. Although the transcriptional programs regulating cell differentiation have been extensively characterized, role of long noncoding RNAs (lncRNAs) in this process remains poorly understood. Using a functional genetic knockdown screen, we identified lncRNA Malat1 as regulator terminal (t-TEM) circulating subset. Evaluation chromatin-enriched lncRNAs...
During a microbial infection, responding CD8+ T cells give rise to effector that provide acute host defense and memory sustained protection. An alternative outcome is exhaustion, state of cell dysfunction occurs in the context chronic infections cancer. Although it evident exhausted (TEX) are phenotypically molecularly distinct from cells, factors regulating earliest events differentiation process TEX remain incompletely understood. Here, we performed single-cell RNA-sequencing...
Differentiating neutrophils undergo large-scale changes in nuclear morphology. How such alterations structure are established and modulated upon exposure to microbial agents is largely unknown. Here, we found that prior encounter with bacteria, an armamentarium of inflammatory genes was positioned a transcriptionally passive environment suppressing premature transcriptional activation. Upon exposure, however, human rapidly (<3 h) repositioned the ensemble proinflammatory toward permissive...
Abstract Maintenance of the regulatory T (Treg) cell pool is essential for peripheral tolerance and prevention autoimmunity. Integrins, heterodimeric transmembrane proteins consisting α β subunits that mediate cell-to-cell cell-to-extracellular matrix interactions, play an important role in facilitating Treg contact–mediated suppression. In this article, we show integrin activation plays essential, previously unappreciated maintaining murine function. cell–specific loss talin, a...
The transition from the follicular B to plasma cell stage is associated with large-scale changes in morphology. Here, we examine whether development also nuclear architecture. We find that onset of concomitant a decline remote genomic interactions; gain euchromatic character at loci encoding for factors specify fate, including Prdm1 and Atf4; establishment de novo inter-chromosomal hubs. that, developing cells concurrent transcriptional silencing, Ebf1 locus repositions an peri-centromeric...
Abstract During an immune response to microbial infection, CD8 + T cells give rise distinct classes of cellular progeny that coordinately mediate clearance the pathogen and provide long-lasting protection against reinfection, including a subset non-circulating tissue-resident memory (T RM ) potent within non-lymphoid tissues. Here, we utilized single-cell RNA-sequencing examine gene expression patterns individual in spleen small intestine intraepithelial lymphocyte (siIEL) compartment...
Abstract The communications industry has a new, forward‐thinking architecture: software‐defined networks (SDN). inflexible structure of conventional severely hinders their ability to service modern organizations. increased adaptability and transparency SDN‐powered makes them more vulnerable security hazards like Distributed denial (DDoS) assaults. switch information system introduces protection at the cost some degree adaptability. software layers are potential entry points for DDoS...
Previous studies have indicated that the transcription signature of antibody-secreting cells is closely associated with induction unfolded protein response pathway (UPR). Here we used genome-wide and single cell analyses to examine folding patterns plasma genomes. We found adopt a cartwheel configuration undergo large-scale changes in chromatin at genomic regions specific signature. During differentiation, Blimp1 assembles into an inter-chromosomal hub genes UPR, biosynthesis endoplasmic...
Abstract Unremitting defense against diverse pathogens and malignancies requires a dynamic durable immune response. Tissue-resident memory CD8 + T cells (T rm ) afford robust protection infection cancer progression through continuous surveillance of non-lymphoid tissues. Here, we provide insight into how confer potent persistent immunity partitioning distinct cellular subsets differing in longevity, effector function, multipotency. Antigen-specific localized to the epithelium small intestine...
Accurately controlling expression of target genes between several designed levels is essential for low-noise gene network and dynamic range expression. However, such manipulations have been hard to achieve due technical limitations. Based on tandem promoters CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) system, we constructed a multistage regulator that could stably regulate the reporter three levels, with more than 2-fold difference each them....
Differentiating neutrophils undergo large-scale changes in nuclear morphology. How such alterations structure are established and modulated upon exposure to microbial agents is largely unknown. Here, we found that prior encounter with bacteria, an armamentarium of inflammatory genes was positioned a transcriptionally passive environment suppressing premature transcriptional activation. Upon exposure, however, human rapidly (<3 hours) repositioned the ensemble pro-inflammatory towards...