A5014591403- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- interferon and immune responses
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Long-Term Effects of COVID-19
- RNA and protein synthesis mechanisms
- Influenza Virus Research Studies
- HIV Research and Treatment
- Virus-based gene therapy research
- RNA Research and Splicing
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Biosensors and Analytical Detection
- Viral Infections and Vectors
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- Mercury impact and mitigation studies
- HIV, Drug Use, Sexual Risk
- HIV/AIDS Research and Interventions
Wuhan University
2016-2025
The First Affiliated Hospital, Sun Yat-sen University
2025
Sun Yat-sen University
2025
Guangdong Academy of Medical Sciences
2025
Guangdong Provincial People's Hospital
2025
Shanghai Ocean University
2007-2024
Wuhan Institute of Virology
2021-2024
Kunsan National University
2023-2024
Air Force Medical University
2024
Chinese People's Liberation Army
2024
The ongoing outbreak of coronavirus disease 2019 (COVID-19) has spread rapidly on a global scale. Although it is clear that severe acute respiratory syndrome 2 (SARS-CoV-2) transmitted through human droplets and direct contact, the potential for aerosol transmission poorly understood1-3. Here we investigated aerodynamic nature SARS-CoV-2 by measuring viral RNA in aerosols different areas two Wuhan hospitals during COVID-19 February March 2020. concentration was detected isolation wards...
Circulating in China and 158 other countries areas, the ongoing COVID-19 outbreak has caused devastating mortality posed a great threat to public health. However, efforts identify effectively supportive therapeutic drugs treatments been hampered by our limited understanding of host immune response for this fatal disease. To characterize transcriptional signatures inflammatory SARS-CoV-2 (HCoV-19) infection, we carried out transcriptome sequencing RNAs isolated from bronchoalveolar lavage...
The article1 to which this Corrigendum refers was published in Journal of Medical Virology 92(4): 418 423 (https://onlinelibrary.wiley.com/doi/full/10.1002/JMV.25681). symptom MHV-A59 should be "Central nervous system disease and hepatitis," rather than "Mouse Acute pneumonia severe lung injuries." authors wish apologize for the error.
From December 2019, an outbreak of unusual pneumonia was reported in Wuhan with many cases linked to Huanan Seafood Market that sells seafood as well live exotic animals. We investigated two patients who developed acute respiratory syndromes after independent contact history this market. The shared common clinical features including fever, cough, and multiple ground-glass opacities the bilateral lung field patchy infiltration. Here, we highlight use a low-input metagenomic next-generation...
The N7-methylguanosine (m7G) cap is the defining structural feature of eukaryotic mRNAs. Most viruses that replicate in cytoplasm, including coronaviruses, have evolved strategies to their RNAs. In this report, we used a yeast genetic system functionally screen for cap-forming enzymes encoded by severe acute respiratory syndrome (SARS) coronavirus and identified nonstructural protein (nsp) 14 SARS as (guanine-N7)-methyltransferase (N7-MTase) vivo cells vitro using purified RNA substrates....
Quantitative real time PCR (RT-PCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. However, due to low viral load specimens and limitations RT-PCR, significant numbers false negative reports are inevitable, which results in failure timely diagnose, cut off transmission, assess discharge criteria. To improve this situation, an optimized droplet digital (ddPCR) was SARS-CoV-2, showed that limit ddPCR significantly lower than RT-PCR. We further explored feasibility...
The 5′-cap structure is a distinct feature of eukaryotic mRNAs, and viruses generally modify the 5′-end viral RNAs to mimic cellular mRNA structure, which important for RNA stability, protein translation immune escape. SARS coronavirus (SARS-CoV) encodes two S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTase) sequentially methylate cap at guanosine-N7 ribose 2′-O positions, catalyzed by nsp14 N7-MTase nsp16 2′-O-MTase, respectively. A unique SARS-CoV that requires...
Abstract Background The ongoing outbreak of COVID-19 has spread rapidly and sparked global concern. While the transmission SARS-CoV-2 through human respiratory droplets contact with infected persons is clear, aerosol been little studied. Methods Thirty-five samples three different types (total suspended particle, size segregated deposition aerosol) were collected in Patient Areas (PAA) Medical Staff (MSA) Renmin Hospital Wuhan University (Renmin) Wuchang Fangcang Field (Fangcang), Public...
The aim of this paper was to monitor the presence SARS-Cov-2 among hospital environment surfaces, sewage, and personal protective equipment (PPE) staffs in isolation wards First Affiliated Hospital Zhejiang University, China.Surfaces objects were routinely wiped with 1000mg/L chlorine containing disinfectant. Air sewage disinfection proceeded strictly. environmental surfaces PPE sampled using swabs. from various inlet outlets sampled. respiratory stool specimens patients collected. also once...
Abstract Background COVID-19 is a viral respiratory disease caused by the severe acute syndrome-Coronavirus type 2 (SARS-CoV-2). Patients with this may be more prone to venous or arterial thrombosis because of activation many factors involved in it, including inflammation, platelet and endothelial dysfunction. Interferon gamma inducible protein-10 (IP-10), monocyte chemoattractant protein-1 (MCP-1) macrophage inflammatory protein 1-alpha (MIP1α) are cytokines related thrombosis. Therefore,...
Abstract Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes the genome of living cells. CXCR4 is a co-receptor for human immunodeficiency virus type 1 (HIV-1) infection been considered as important therapeutic target AIDS. mediates viral entry into CD4 + cells by binding envelope protein, gp120. Here, we show that gene efficiently disrupted CRISPR/Cas9-mediated editing, leading HIV-1 resistance primary T We also Cas9-mediated ablation...
RNA interference (RNAi) is a process of eukaryotic posttranscriptional gene silencing that functions in antiviral immunity plants, nematodes, and insects. However, recent studies provided strong supports RNAi also plays role mechanism mammalian cells. To combat RNAi-mediated responses, many viruses encode viral suppressors (VSR) to facilitate their replication. VSRs have been widely studied for plant insect viruses, but only few defined currently. We identified novel VSR from coronaviruses,...
Abstract Emerging and re-emerging RNA viruses occasionally cause epidemics pandemics worldwide, such as the on-going outbreak of novel coronavirus SARS-CoV-2. Herein, we identified two potent inhibitors human DHODH, S312 S416, with favorable drug-likeness pharmacokinetic profiles, which all showed broad-spectrum antiviral effects against various viruses, including influenza A virus, Zika Ebola particularly Notably, S416 is reported to be most inhibitor so far an EC 50 17 nmol/L SI value...
Summary The sudden outbreak of the severe acute respiratory syndrome–coronavirus (SARS-CoV-2) has spread globally with more than 1,300,000 patients diagnosed and a death toll 70,000. Current genomic survey data suggest that single nucleotide variants (SNVs) are abundant. However, no mutation been directly linked functional changes in viral pathogenicity. We report characterizations 11 patient-derived isolates. observed diverse mutations these isolates, including 6 different spike...
Postmeiotic spermatids use a unique strategy to coordinate gene expression with morphological transformation, in which transcription and translation take place at separate developmental stages, but how mRNAs stored as translationally inert messenger ribonucleoproteins developing become activated remains largely unknown. Here, we report that the RNA binding protein FXR1, member of fragile X-related (FXR) family, is highly expressed late undergoes liquid-liquid phase separation (LLPS) merge...
Middle East respiratory syndrome coronavirus (MERS-CoV) and several bat coronaviruses use dipeptidyl peptidase-4 (DPP4) as an entry receptor1-4. However, the receptor for NeoCoV-the closest known MERS-CoV relative found in bats-remains unclear5. Here, using a pseudotype virus assay, we that NeoCoV its close relative, PDF-2180, can efficiently bind to specific angiotensin-converting enzyme 2 (ACE2) orthologues and, less favourably, human ACE2 receptors through their receptor-binding domains...
ABSTRACT The expression of the genomic information severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis a nested set subgenomic RNAs (sgRNAs) by discontinuous transcription. In SARS CoV-infected cells, 10 sgRNAs, including 2 novel ones, were identified, which predicted to be functional in 12 open reading frames located 3′ one-third genome. Surprisingly, one new sgRNA could lead production truncated spike protein. Sequence analysis leader-body fusion sites each showed...