A5100427227- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Autophagy in Disease and Therapy
- CAR-T cell therapy research
- Inflammasome and immune disorders
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Natural product bioactivities and synthesis
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Viral Infections and Immunology Research
- COVID-19 Clinical Research Studies
- NF-κB Signaling Pathways
- Pharmacological Effects of Natural Compounds
- PI3K/AKT/mTOR signaling in cancer
- Ocular Diseases and Behçet’s Syndrome
- Plant Diversity and Evolution
- Systemic Lupus Erythematosus Research
- Viral Infections and Vectors
- Endoplasmic Reticulum Stress and Disease
Sun Yat-sen University
2014-2025
Shenzhen University
2025
Yantai University
2011-2024
Biocon (Switzerland)
2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2020-2021
Anhui Medical University
2018
Wuhan University
2017-2018
Air Force Medical University
2018
Xijing Hospital
2018
Simon Fraser University
2016
The main approach to treat HIV-1 infection is combination antiretroviral therapy (cART). Although cART effective in reducing viral load and controlling disease progression, it has many side effects, expensive for infected patients who must remain on lifetime treatment. gene drawn much attention as studies of genome editing tools have progressed. For example, zinc finger nucleases (ZFN), transcription activator like effector (TALEN) clustered regularly interspaced short palindromic repeats...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus driving ongoing disease 2019 (COVID-19) pandemic, continues to rapidly evolve. Because of limited efficacy vaccination in prevention SARS-CoV-2 transmission and continuous emergence variants concern (VOCs), orally bioavailable broadly efficacious antiviral drugs are urgently needed. Previously, we showed that parent nucleoside remdesivir, GS-441524, has potent anti-SARS-CoV-2 activity. Here, report esterification...
The CRISPR/Cas9 system has been widely used for genome editing in mammalian cells. CXCR4 is a co-receptor human immunodeficiency virus type 1 (HIV-1) entry, and loss of function can protect cells from (X4)-tropic HIV-1 infection, making an important target gene therapy. However, the large size CRISPR/SpCas9 presents obstacle to its efficient delivery into primary CD4+ T Recently, small Staphylococcus aureus Cas9 (SaCas9) developed as tool address this question. Therefore, it provides...
Ferroptosis is a newly discovered form of regulated cell death characterized by increased intracellular iron accumulation and subsequent lipid peroxidation (Dixon et al., 2012).Studies have revealed that ferroptosis plays an important role in multiple physiological pathological processes including degenerative diseases, carcinogenesis, cancer immunotherapy (Hassannia 2019, Wang 2019).As storage protein, ferritin degraded via the selective autophagy-mediated degradation process named...
The C-X-C chemokine receptor type 4 (CXCR4) is one of the major co-receptors for human immunodeficiency virus 1 (HIV-1) entry and considered an important therapeutic target. However, its function in maintaining development hematopoietic stem cells (HSC) makes it difficult to be used HIV-1 gene therapy with HSC transplantation. A previous report showed that natural CXCR4 P191A mutant inhibits infection without any defect differentiation, which could provide a basis new approaches therapy. In...
Abstract Glioblastoma (GBM) is among the most aggressive human cancers. Although oncolytic virus (OV) therapy has been proposed as a potential approach to treat GBM, it frequently fails because GBM cells are usually nonpermissive OV. Here, we describe dual-step drug screen for identifying chemical enhancers of OV in GBM. From high-throughput 1416 FDA-approved drugs, an inhibitor CDK4/6 was identified top enhancer, selectively increasing potency two strains, VSVΔ51 and Zika virus....
Interferon-inducible p200 family protein IFI204 was reported to be involved in DNA sensing, and subsequently induces the production of type I interferons proinflammatory mediators. However, its function regulation antiviral innate immune signaling pathway remains unclear. Here we a novel role that specifically inhibits IRF7-mediated response during viral infection. other proteins are highly expressed mouse hepatitis coronavirus-infected bone marrow-derived dendritic cells. The abundant could...
Highlights•Active disease in uveitis patients is associated with reduced serum IFN-α•Effective treatment of type I IFN requires CXCR3 signaling•CXCR3 engagement retains pathogenic CD4+ T cells peripheral lymphoid organs•CXCR3 may be a potential biomarker to predict the efficacy for therapySummaryType interferons (IFNs) have therapeutic CNS autoimmune diseases, such as uveitis, but molecular mechanisms remain unclear. Using cell-transfer model experimental (EAU), we found that IFN-α/β...
Nuclear factor κB (NF-κB)-mediated signaling pathway plays a crucial role in the regulation of inflammatory process, innate and adaptive immune responses. The hyperactivation response causes host cell death, tissue damage, autoinflammatory disorders, such as sepsis bowel disease. However, how these processes are precisely controlled is still poorly understood. In this study, we demonstrated that ankyrin repeat suppressor cytokine box containing 1 (ASB1) involved positive responses by...
Protein aggregation caused by the disruption of proteostasis will lead to cellular cytotoxicity and even cell death, which is implicated in multiple neurodegenerative diseases. The elimination aggregated proteins mediated selective macroautophagy receptors, termed aggrephagy. However, identity redundancy aggrephagy receptors recognizing substrates remain largely unexplored. Here, we find that CCDC50, a highly expressed autophagy receptor brain, recruited proteotoxic stresses-induced...
Coronaviruses (CoVs) pose a significant threat to human health, as demonstrated by the COVID-19 pandemic. The large size of CoV genome (around 30 kb) represents major obstacle development reverse genetics systems, which are invaluable for basic research and antiviral drug screening. In this study, we established rapid convenient method generating genetic systems various CoVs using bacterial artificial chromosome (BAC) vector Gibson DNA assembly. Using system, constructed infectious cDNA...
The precise regulation of the RIG-I-like receptors (RLRs)-mediated type I interferon (IFN-I) activation is crucial in antiviral immunity and maintaining host immune homeostasis meantime. Here, we identify an E3 ubiquitin ligase, namely RNF167, as a negative regulator RLR-triggered IFN signaling. Mechanistically, RNF167 facilitates both atypical K6- K11-linked polyubiquitination RIG-I/MDA5 within CARD CTD domains, respectively, which leads to degradation viral RNA sensors through dual...
Glycyrrhiza glabra has been used in medicine for thousands of years. Our previous study revealed that the methanolic extract L. (EGGR) exhibits significant nitric oxide (NO) inhibitory effect on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages among 100 other extracts. Accordingly, aim present was to investigate potential anti-inflammatory EGGR. The EGGR LPS-stimulated measured by MTT assay, NO content analysis, reactive oxygen species (ROS) level RT-PCR, Western blot and ELISA...
Innate immunity is the first-line defense against antiviral or antimicrobial infection. RIG-I and MDA5, which mediate recognition of pathogen-derived nucleic acids, are essential for production type I interferons (IFN). Here we identified mitochondrion depolarization inducer carbonyl cyanide 3-chlorophenylhydrazone (CCCP) inhibited response activity IFN during viral Furthermore, found that PTEN-induced putative kinase 1 (PINK1) E3 ubiquitin-protein ligase Parkin mediated mitophagy, thus...
The assembly of the NLRP3 inflammasome can be initiated by a wide range stimuli including exogenous infection as well endogenous damage. Therefore, tight regulation is crucial for host to resist microbial invasion and maintain homeostasis. Our recent work has identified negative regulator NLRP3-mediated inflammation, namely CCDC50 (coiled-coil domain containing protein 50). induced agonists then functions macroautophagy/autophagy cargo receptor recognize K63-polyubiquitinated deliver it...