A5081518479- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Single-cell and spatial transcriptomics
- Inflammatory Bowel Disease
- Immune cells in cancer
- Microscopic Colitis
- RNA Interference and Gene Delivery
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Cancer Immunotherapy and Biomarkers
- Legume Nitrogen Fixing Symbiosis
- Dermatology and Skin Diseases
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
University of California, San Diego
2018-2023
Salk Institute for Biological Studies
2023
California State University, San Marcos
2015
Single-cell sequencing analyses reveal changes in B and T cell transcriptional states the context of ulcerative colitis.
Heterogeneity of small intestine intraepithelial CD8 + T cells suggests functionally distinct subsets RM and their precursors.
Nitrogen is an essential soil nutrient for plants, and lack of nitrogen commonly limits plant growth. Soil typically available to plants in two inorganic forms: nitrate ammonium. To better understand how ammonium differentially affect metabolism development, we performed transcriptional profiling the shoots ammonium-supplied nitrate-supplied Arabidopsis (Arabidopsis thaliana) plants. Seven genes encoding class III glutaredoxins were found be strongly specifically induced by nitrate. RNA...
During an immune response, CD8+ T lymphocytes can undergo asymmetric division, giving rise to daughter cells that exhibit distinct tendencies adopt terminal effector and memory cell fates. Here we show “pre-effector” “pre-memory” resulting from the first division in vivo exhibited low high rates of endogenous proteasome activity, respectively. Pharmacologic reduction activity early during differentiation resulted acquisition characteristics, whereas enhancement conferred attributes...
Abstract Maintenance of the regulatory T (Treg) cell pool is essential for peripheral tolerance and prevention autoimmunity. Integrins, heterodimeric transmembrane proteins consisting α β subunits that mediate cell-to-cell cell-to-extracellular matrix interactions, play an important role in facilitating Treg contact–mediated suppression. In this article, we show integrin activation plays essential, previously unappreciated maintaining murine function. cell–specific loss talin, a...
Inflammatory disorders of the skin are frequently associated with inflammatory bowel diseases (IBDs). To explore mechanisms by which these organs communicate, we performed single-cell RNA-Seq analysis on fibroblasts from humans and mice IBD. This revealed that intestinal inflammation promoted differentiation a subset stromal into preadipocytes innate antimicrobial host defense activity. Furthermore, this process reactive adipogenesis was exacerbated if mouse inflamed as result wounding or...
Abstract Background Ulcerative colitis (UC) and Crohn’s disease are 2 types of inflammatory bowel (IBD), a group chronic digestive disorders caused by aberrant immune responses to intestinal microbes. Although changes in the composition cell subsets context IBD have been previously described, interactions communication among cells less well understood. Moreover, precise mechanisms action underlying many biologic therapies, including anti-α4β7 integrin antagonist vedolizumab, remain...
ABSTRACT INTRODUCTION: Crohn’s disease (CD) is a major subtype of inflammatory bowel (IBD), spectrum chronic intestinal disorders caused by dysregulated immune responses to gut microbiota. Although transcriptional and functional changes in number cell types have been implicated the pathogenesis IBD, cellular interactions signals that drive these less well-studied. METHODS: We performed Cellular Indexing Transcriptomes Epitopes sequencing (CITE-seq) on peripheral blood, colon, ileal cells...
Abstract During an immune response to microbial infection, CD8 + T cells give rise distinct classes of cellular progeny that coordinately mediate clearance the pathogen and provide long-lasting protection against reinfection, including a subset non-circulating tissue-resident memory (T RM ) potent within non-lymphoid tissues. Here, we utilized single-cell RNA-sequencing examine gene expression patterns individual in spleen small intestine intraepithelial lymphocyte (siIEL) compartment...
ABSTRACT We used DNA origami to create NanoNERF, the world’s smallest NERF blaster replica (Figure 1). based our design on model Maverick Rev-6 , and scaled dimensions down three million times. NanoNERF is planar measures ∼100 nm in length, with a length-to-width ratio closely resembling original toy. Here, we describe design, prototyping, validation pipeline NanoNERF. also discuss potential applications motivate creation of future nanoscale blasters firing functionality.