A5006427512- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- TGF-β signaling in diseases
- Ubiquitin and proteasome pathways
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Inflammatory Bowel Disease
- Immune cells in cancer
- SARS-CoV-2 and COVID-19 Research
- Cancer-related molecular mechanisms research
- Mycobacterium research and diagnosis
- Vaccine Coverage and Hesitancy
- COVID-19 Clinical Research Studies
- RNA Research and Splicing
University of California, San Diego
2018-2023
Single-cell sequencing analyses reveal changes in B and T cell transcriptional states the context of ulcerative colitis.
Heterogeneity of small intestine intraepithelial CD8 + T cells suggests functionally distinct subsets RM and their precursors.
During an immune response to microbial infection, CD8+ T cells give rise short-lived effector and memory that provide sustained protection. Although the transcriptional programs regulating cell differentiation have been extensively characterized, role of long noncoding RNAs (lncRNAs) in this process remains poorly understood. Using a functional genetic knockdown screen, we identified lncRNA Malat1 as regulator terminal (t-TEM) circulating subset. Evaluation chromatin-enriched lncRNAs...
Abstract Epithelial–mesenchymal transition (EMT) is a conserved cellular plasticity program that reactivated in carcinoma cells and drives metastasis. Although EMT well studied its regulatory mechanisms remain unclear. Therefore, to identify novel regulators of EMT, data mining approach was taken using published microarray group deubiquitinases (DUB) were found be upregulated have undergone EMT. Here, it demonstrated one DUB, ubiquitin-specific peptidase 11 (USP11), enhances TGFβ-induced...
During a microbial infection, responding CD8+ T cells give rise to effector that provide acute host defense and memory sustained protection. An alternative outcome is exhaustion, state of cell dysfunction occurs in the context chronic infections cancer. Although it evident exhausted (TEX) are phenotypically molecularly distinct from cells, factors regulating earliest events differentiation process TEX remain incompletely understood. Here, we performed single-cell RNA-sequencing...
Abstract Maintenance of the regulatory T (Treg) cell pool is essential for peripheral tolerance and prevention autoimmunity. Integrins, heterodimeric transmembrane proteins consisting α β subunits that mediate cell-to-cell cell-to-extracellular matrix interactions, play an important role in facilitating Treg contact–mediated suppression. In this article, we show integrin activation plays essential, previously unappreciated maintaining murine function. cell–specific loss talin, a...
Immune-modulating medications for inflammatory bowel diseases (IBDs) have been associated with suboptimal vaccine responses. There are conflicting data SARS-CoV-2 vaccination. We therefore assessed immunogenicity at 2 weeks after second mRNA vaccination in 29 patients IBD compared 12 normal healthy donors. observed reduced humoral immunity on infliximab. However, we no difference and cell-mediated infliximab a thiopurine or vedolizumab This is the first study to demonstrate comparable...
Abstract The RNA-binding protein DEAD-box 5 (DDX5) is a polyfunctional regulator of gene expression, but its role in CD8+ T cell biology has not been extensively investigated. In this study, we demonstrate that deletion DDX5 murine cells reduced the differentiation terminal effector, effector memory T, and while increasing generation central cells, whereas forced expression elicited opposite phenotype. DDX5-deficient exhibited increased genes promote differentiation, including Tcf7 Eomes....
Abstract During an immune response to microbial infection, CD8 + T cells give rise distinct classes of cellular progeny that coordinately mediate clearance the pathogen and provide long-lasting protection against reinfection, including a subset non-circulating tissue-resident memory (T RM ) potent within non-lymphoid tissues. Here, we utilized single-cell RNA-sequencing examine gene expression patterns individual in spleen small intestine intraepithelial lymphocyte (siIEL) compartment...
<p>Supp. Fig. 1. High USP11 expression correlates with decreased survival in breast cancer patients. Supp. 2. Validation of retroviral overexpression and shRNA knockdown select DUBs. 3. regulates self-renewal normal human epithelial cells. 4. is upregulated cell lines. 5. SUM159 transwell migration TGF-beta-dependent. 6. TGF-beta induced EMT markers lines.</p>
<div>Abstract<p>Epithelial–mesenchymal transition (EMT) is a conserved cellular plasticity program that reactivated in carcinoma cells and drives metastasis. Although EMT well studied its regulatory mechanisms remain unclear. Therefore, to identify novel regulators of EMT, data mining approach was taken using published microarray group deubiquitinases (DUB) were found be upregulated have undergone EMT. Here, it demonstrated one DUB, ubiquitin-specific peptidase 11 (USP11),...
<div>Abstract<p>Epithelial–mesenchymal transition (EMT) is a conserved cellular plasticity program that reactivated in carcinoma cells and drives metastasis. Although EMT well studied its regulatory mechanisms remain unclear. Therefore, to identify novel regulators of EMT, data mining approach was taken using published microarray group deubiquitinases (DUB) were found be upregulated have undergone EMT. Here, it demonstrated one DUB, ubiquitin-specific peptidase 11 (USP11),...
<p>Supp. Fig. 1. High USP11 expression correlates with decreased survival in breast cancer patients. Supp. 2. Validation of retroviral overexpression and shRNA knockdown select DUBs. 3. regulates self-renewal normal human epithelial cells. 4. is upregulated cell lines. 5. SUM159 transwell migration TGF-beta-dependent. 6. TGF-beta induced EMT markers lines.</p>