A5059048178- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Immune Cell Function and Interaction
- Parasites and Host Interactions
- Toxin Mechanisms and Immunotoxins
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Immune cells in cancer
- Growth Hormone and Insulin-like Growth Factors
- Reproductive System and Pregnancy
- Biomarkers in Disease Mechanisms
- Parasitic Infections and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Autoimmune and Inflammatory Disorders Research
- Eosinophilic Disorders and Syndromes
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Axon Guidance and Neuronal Signaling
- Neonatal Respiratory Health Research
- SARS-CoV-2 and COVID-19 Research
- Synthesis and Biological Evaluation
- Transgenic Plants and Applications
- Macrophage Migration Inhibitory Factor
- Pharmacological Effects of Natural Compounds
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
University of Manitoba
2016-2025
Newcastle University
2015
University of Pennsylvania
2001-2005
University of Saskatchewan
1997-2001
Infection of susceptible BALB/c mice with a large, moderate, or low number Leishmania major parasites respectively results in progressive disease, the formation substantial but stable lesions, denoted as borderline and absence visible lesion. over long term either subclinical infections an asymptomatic state. Subclinical produce predominant Th1 response are resistant to challenge, contrast their counterparts. Statistical other evidence suggest that state can arise from following parasite...
An unresolved issue in the field of T helper (Th) cell development relates to findings that low doses antigen promote Th2 vitro, whereas several classic vivo studies suggest opposite. Here we resolve this paradox by studying early immune response mice after infection with different Leishmania major. We found parasite induced a C57BL/6 (B6) mice, high Th1 response. However, dose–infected was transient and animals healed. The appearance dose dependent upon concomitant activation interferon...
Abstract Long-term immunity to Leishmania may require the continued presence of parasites, but previous attempts create attenuated parasites that persist without causing disease have had limited success. Since major mutants lack lipophosphoglycan and other secreted phosphoglycans, termed lpg2−, indefinitely in infected mice inducing any disease, we tested their ability provide protection virulent L. challenge. In response leishmanial Ag stimulation, cells from lpg2−-infected produced minimal...
Resistance to Leishmania major and most intracellular pathogens is usually associated with a strong T cell-mediated immunity, particularly CD4(+) Th1 response. Mice an inactivating knock-in mutation in the p110delta isoform of PI3K (referred as p110delta(D910A)) show severely impaired cell responses. Because response thought mediate resistance pathogens, we examined outcome L. infection p110delta(D910A) mice. Paradoxically, mice on "resistant" "susceptible" genetic backgrounds showed more...
Background Trypanosoma congolense are extracellular and intravascular blood parasites that cause debilitating acute or chronic disease in cattle other domestic animals. Diminazene aceturate (Berenil) has been widely used as a chemotherapeutic agent for trypanosomiasis livestock since 1955. As livestock, treatment of infected highly susceptible BALB/c mice with Berenil leads to rapid control parasitemia survival from an otherwise lethal infection. The molecular biochemical mechanisms action...
A Leishmania protein vaccine elicited immune responses and mediated strong cross-species protection.
The balance between the products of L-arginine metabolism in macrophages regulates outcome Leishmania major infection. can be oxidized by host inducible NO synthase to produce NO, which contributes parasite killing. In contrast, hydrolysis arginase blocks generation and provides polyamines, support proliferation. Additionally, encode their own has considerable potential modulate infectivity disease pathogenesis. this study, we compared impact on cellular immune response vitro vivo wild-type...
It is well established that CD4CD25 regulatory T cells (Tregs) downregulate inflammatory immune responses and help to maintain homeostasis. Recent reports have shown ligation of germline encoded pattern recognition receptors such as Toll-like can stimulate Tregs therefore implicate in the pathophysiology sepsis other diseases. In this report, we show injection lipopolysaccharide (LPS) leads expansion CD4CD25FoxP3 Tregs, suggesting these may play an important role regulation LPS-induced acute...
Although a great deal of knowledge has been gained from studies on the immunobiology leishmaniasis, there is still no effective vaccine against disease. This strongly suggests that we do not understand factors control and/or regulate development and sustenance anti-Leishmania immunity, particularly those associated with secondary (memory) immunity. Such an understanding critically important for designing vaccines Here review literature correlate protective immunity vaccination strategies...
Although diminazene aceturate (Berenil) is widely used as a trypanolytic agent in livestock, its mechanisms of action remain poorly understood. We previously showed that Berenil treatment suppresses pro-inflammatory cytokine production by splenic and liver macrophages leading to concomitant reduction serum levels mice infected with Trypanosoma congolense or challenged LPS. Here, we investigated the molecular through which alters macrophages. show pre-treatment dramatically suppressed IL-6,...