A5028424524- Glaucoma and retinal disorders
- DNA Repair Mechanisms
- Retinal Imaging and Analysis
- Advanced Fluorescence Microscopy Techniques
- Corneal surgery and disorders
- Retinal Diseases and Treatments
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Optical Coherence Tomography Applications
- Microfluidic and Bio-sensing Technologies
- Axon Guidance and Neuronal Signaling
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Photoreceptor and optogenetics research
- Intraocular Surgery and Lenses
- Retinal Development and Disorders
- Cellular transport and secretion
- Retinopathy of Prematurity Studies
- Optical Imaging and Spectroscopy Techniques
- Nanofabrication and Lithography Techniques
- Ocular Surface and Contact Lens
- Genomics and Chromatin Dynamics
- Force Microscopy Techniques and Applications
- Calcium signaling and nucleotide metabolism
- Immunotherapy and Immune Responses
Hôpital Maisonneuve-Rosemont
2015-2024
Université de Montréal
2015-2024
Centre Intégré Universitaire de Santé et de Services Sociaux du Centre-Sud-de-l'Île-de-Montréal
2019-2022
McGill University
2005-2012
Institut National de la Recherche Scientifique
2012
Johns Hopkins Medicine
2011
Johns Hopkins University
2011
Université Laval
2009
Montreal Neurological Institute and Hospital
2007-2008
University of Buenos Aires
2000-2004
Packaging DNA into condensed structures is integral to the transmission of genomes. The mammalian mitochondrial genome (mtDNA) a high copy, maternally inherited in which mutations cause variety multisystem disorders. In all eukaryotic cells, multiple mtDNAs are packaged with protein spheroid bodies called nucleoids, fundamental units mtDNA segregation. mechanism nucleoid formation, however, remains unknown. Here, we show that transcription factor TFAM, an abundant and highly conserved High...
Melanoma is an immunogenic cancer with a high response rate to immune checkpoint inhibitors (ICIs). It harbors mutation burden compared other cancers and, as result, has abundant tumor-infiltrating lymphocytes (TILs) within its microenvironment. However, understanding the complex interplay between stroma, tumor cells, and distinct TIL subsets remains substantial challenge in oncology. To properly study this interplay, quantifying spatial relationships of multiple cell types microenvironment...
Measuring protein interactions is key to understanding cell signaling mechanisms, but quantitative analysis of these in situ has remained a major challenge. Here, we present spatial intensity distribution (SpIDA), an technique for image data obtained using standard fluorescence microscopy. SpIDA directly measures fluorescent macromolecule densities and oligomerization states sampled within single images. The method based on fitting histograms calculated from images obtain density maps...
C-reactive protein (CRP) has been implicated in the regulation of inflammation underlying coronary artery disease; however, little is known about molecular mechanisms responsible for expression its pro- or anti-inflammatory activities. Here, we have identified intrasubunit disulfide bond as a conserved switch that controls structure and functions CRP. Conformational rearrangement human pentameric CRP to monomeric (mCRP) prerequisite this be activated by reducing agents, including...
Mechanisms regulating B cell development, activation, education in the germinal center (GC) and differentiation, underpin humoral immune response. Protein arginine methyltransferase 5 (Prmt5), which catalyzes most symmetric dimethyl protein modifications, is overexpressed lymphomas but its function normal cells poorly defined. Here we show that Prmt5 necessary for antibody responses has essential distinct functions all proliferative stages mice. development by preventing p53-dependent...
Adoptive T cell therapies rely on the production of cells with an antigen receptor that directs their specificity toward tumor-specific antigens. Methods for identifying relevant (TCR) sequences, predominantly achieved through enrichment antigen-specific cells, represent a major bottleneck in TCR-engineered therapies. Fluctuation intracellular calcium is proximal readout TCR signaling and candidate marker identification does not require expansion; however, fluctuations downstream engagement...
Mutations in the gene encoding CLN5 are cause of Finnish variant late infantile Neuronal Ceroid Lipofuscinosis (NCL), and is 1 10 genes (encoding CLN1 to CLN9 cathepsin D) whose germ line mutations result a group recessive disorders childhood. Although localizes lysosomal compartment, its function remains unknown. We have uncovered an interaction between sortilin, sorting receptor. However, CLN5, unlike prosaposin, does not require sortilin localize compartment. demonstrate that...
Abstract The use of optical coherence tomography (OCT) to study ocular diseases associated with choroidal physiology is sharply limited by the lack available automated segmentation tools. Current research largely relies on hand-traced, single B-Scan segmentations because commercially programs require high quality images, and existing implementations are closed, scarce not freely available. We developed implemented a robust algorithm for segmenting quantifying layer from 3-dimensional OCT...
Abstract Intrinsic and acquired resistance to cisplatin remains a primary hurdle treatment of high-grade serous ovarian cancer (HGSOC). Cisplatin selectively kills tumor cells by inducing DNA crosslinks that block replicative polymerases. Single-stranded (ssDNA) generated at resulting stalled replication forks (RF) is bound protected heterotrimeric protein A (RPA), which then serves as platform for recruitment activation stress response factors. Cells deficient in this are characterized...
Vision loss caused by retinal diseases affects hundreds of millions individuals worldwide. The retina is a delicate central nervous system tissue stratified into layers cells with distinct roles. Currently, there void in treatments that selectively target diseased cells, and current therapeutic paradigms present complications associated off-target effects. Herein, as proof concept, we introduce an vivo method using femtosecond laser to locally optoporate ganglion (RGCs) targeted...
The study of cellular responses to changes in the spatial distribution molecules development, immunology and cancer, requires reliable methods reproduce vitro precise distributions proteins found vivo. Here we present a straightforward method for generating substrate-bound protein patterns which has simplicity required be implemented typical life science laboratories. exploits photobleaching fluorescently tagged generate concentration gradients with sub-micron resolution. We provide an...
Axonal injury and degeneration are pivotal pathological events in diseases of the nervous system. In past decade, it has been recognized that process axonal is distinct from somal axoprotective strategies may be those protect soma. Preserving cell body via neuroprotection cannot improve function if axon damaged, because soma still disconnected its target. Therefore, understanding mechanisms critical for developing new therapeutic interventions disease treatment. We combined vivo imaging with...
Sorting from the Golgi apparatus requires recruitment of cytosolic coat proteins to package cargo into trafficking vesicles. An important early step in formation vesicles is activation Arf1 by guanine nucleotide exchange factor GBF1. To activate Arf1, GBF1 must be recruited and bound membranes, a process that Rab1b. However, mechanistic details how Rab1 implicated are not known. In this study, we demonstrate also phosphatidylinositol 4-phosphate [PtdIns(4)P]. Inhibitors PtdIns(4)P synthesis...
We have developed a novel optical approach to determine pulsatile ocular volume changes using automated segmentation of the choroid, which, together with Dynamic Contour Tonometry (DCT) measurements intraocular pressure (IOP), allows estimation rigidity (OR) coefficient. Spectral Domain Optical Coherence Tomography (OCT) videos were acquired Enhanced Depth Imaging (EDI) at 7Hz during ~50 seconds fundus. A algorithm based on graph search an edge-probability weighting scheme was measure...
Cells sense spatial distributions of molecules which trigger signal transduction pathways that induce the cell to migrate or extend by remodelling cytoskeleton. However, influence local and small variations extracellular protein concentration on chemotaxis is not fully understood, due in part lack simple precise methods pattern proteinsin vitro. We recently developed a new technology fabricate such patterns relies photobleaching fluorophores adsorb proteins culture substrate: laser-assisted...