A5018173554- Retinal Development and Disorders
- Retinopathy of Prematurity Studies
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- Circadian rhythm and melatonin
- Neonatal and fetal brain pathology
- Birth, Development, and Health
- Photoreceptor and optogenetics research
- Neonatal Respiratory Health Research
- Ocular and Laser Science Research
- Retinoids in leukemia and cellular processes
- Peroxisome Proliferator-Activated Receptors
- Neural dynamics and brain function
- Preterm Birth and Chorioamnionitis
- Eicosanoids and Hypertension Pharmacology
- melanin and skin pigmentation
- CRISPR and Genetic Engineering
- Medical and Biological Ozone Research
- Retinal Imaging and Analysis
- Zebrafish Biomedical Research Applications
- Optical Imaging and Spectroscopy Techniques
- Axon Guidance and Neuronal Signaling
- Pluripotent Stem Cells Research
- Antioxidant Activity and Oxidative Stress
McGill University Health Centre
2015-2025
Montreal Children's Hospital
2009-2025
Hôpital Maisonneuve-Rosemont
2021-2024
McGill University
2009-2021
Purpose.: Perinatal inflammatory stress in preterm babies is associated with increased rates of severe retinopathy prematurity (ROP) and adverse neurological dysfunction. In this study, we set out to determine the consequences systemic on developmental retinal vascularization evaluate subsequent outcome function later life. Methods.: Systemic was induced C57BL/6J mouse pups by an intraperitoneal injection lipopolysaccharide (LPS; 1 mg/kg) at postnatal day 4. response LPS, inflammation...
Purpose.: Retinopathy of prematurity (ROP) is a major cause visual handicap in the pediatric population. To date, this disorder thought to stem from deficient retinal vascularization. Intriguingly, functional electrophysiological studies patients with mild or moderate ROP and oxygen-induced retinopathy (OIR) model rats reveal central photoreceptor disruption that overlies modest vessel loss; paucity vasculature occurs predominantly at periphery. Given choroidal circulation source oxygen...
Purpose Biological sex and age are considered as two important factors that may influence the function structure of retina, an effect might be governed by sexual hormones such estrogen. The purpose this study was to delineate biological exert on retinal rodents also clarify estrus cycle female rats. Method 50 normal male albino Sprague-Dawley (SD) rats investigated with electroretinogram (ERG) at postnatal day (P) 30, 60, 100, 200, 300 (n = 5–6 rats/age). Following ERG recording sessions,...
The purpose of this study was to better understand the role ocular pigmentation and genetics in light-induced retinal damage. Adult pigmented [Long Evans (LE) Brown Norway (BN)] albino [Sprague Dawley (SD) Lewis (LW)] rats were exposed a bright cyclic light for 6 consecutive days where compared with juvenile animals same environment from postnatal age 14 28. Flash ERGs histology performed at predetermined (D) post-light exposure. At D1, similar all adult groups no recordable a-waves residual...
Results of studies that compared the racial incidence retinopathy prematurity (ROP) suggested ocular pigmentation might offer protection against development severe ROP. The structural and functional consequences postnatal hyperoxia (oxygen-induced retinopathy; OIR) were in albino Sprague-Dawley (SD) pigmented Long-Evans (LE) rats to verify this finding.Newborn exposed 80% O(2) during selected day intervals. severity OIR was determined by examining retinal flatmounts (retinal vasculature...
Purpose: We investigated the effects of term neonatal encephalopathy on retinal function and structure. Methods: A rat model hypoxic-ischemic (HI) (Vannucci model) was used. Hypoxia-ischemia induced by a left common carotid ligation followed 2-hour period hypoxia (8% oxygen) in Long-Evans pups at postnatal day 10 (P10). Sham operated rats served as controls.. Retinal assessed P30 P60 electroretinograms (ERGs), after which histology performed. Retinocortical with visual evoked potentials...
ABSTRACT Retinal degenerative diseases affect millions of people worldwide, and legal blindness is generally associated with the loss cone photoreceptors located in central region retina called macula. Currently, there no treatment to replace Addressing this unmet need, we employed control isogenic hypoimmunogenic induced pluripotent stem cell lines generate spontaneously polarized retinal sheets (RSs). RSs were enriched progenitor precursor cells, which could differentiate into mature S-...
Patients with Zellweger spectrum disorder (ZSD) commonly present vision loss due to mutations in PEX genes required for peroxisome assembly and function. Here, we evaluate PEX1 retinal gene augmentation therapy a mouse model of mild ZSD bearing the murine equivalent (PEX1-p[Gly844Asp]) most common human mutation. Experimental adeno-associated virus 8.cytomegalovirus.human PEX1.hemagglutinin (AAV8.CMV.HsPEX1.HA) control AAV8.CMV.EGFP vectors were administered by subretinal injection...
Purpose: To compare the retinal function of a diurnal murid rodent, Psammomys obesus, with that Wistar albino rat and human subjects.Materials methods: Adult obesus were captured transferred to animal facilities where they maintained at 25°C standard light/dark cycles natural halophilic plants, rich in water mineral salts. Standard full-field photopic scotopic electroretinograms obtained.Results: The right eye all animals displayed well detectable reproducible electroretinogram (ERG)...
Purpose: The purpose of this study was to compare the efficacy that ocular melanin confers in protecting retina juvenile and adult rats exposed a bright luminous environment.Methods: Juvenile (JLE) (ALE) Long–Evans pigmented were thus cyclic light (10,000lux; white light) from postnatal day 14–28 or for 6 consecutive days, respectively. Flash electroretinograms (ERG) retinal histology performed at different predetermined ages, post-light exposure.Results: Despite significant reduction ERG...
In the present study, we aimed at better understanding short (acute) and long term (chronic) degenerative processes characterizing juvenile rat model of light-induced retinopathy. Electroretinograms, visual evoked potentials (VEP), retinal histology western blots were obtained from albino Sprague-Dawley rats preselected postnatal ages (from P30 to P400) following exposure 10,000 lux P14 P28. Our results show that while immediately cessation exposure, photoreceptor degeneration was...
The electroretinogram (ERG) represents the biopotential evoked by retina in response to a light stimulus. flash ERG (fERG) is modality most frequently used clinically diagnose and monitor retinal disorders. We hereby present new method record spontaneous activity, without use of stimulus, that we named resting-state (rsERG). recordings were done normal subjects under light- dark-adaptation with different background conditions (i.e., variations wavelength intensity). Additionally, rsERG...
To unravel the mechanisms behind higher resistance to light damage of juvenile (JR) versus adult (AR) rats, Sprague Dawley rats were exposed a bright luminous environment 10, 000 lux. The light-induced retinopathy (LIR) was assessed with histology, electroretinography and immunohistochemistry (IHC). In JR, 2 days exposure induced typical LIR, while >3 added little LIR. IHC revealed subtle migration microglia (Iba1 marker) from inner outer retina after 3 in JR contrasting stronger reaction...
ABSTRACT Retinal degenerative diseases affect millions of people worldwide, and legal blindness is generally associated with the loss cone photoreceptors located in retina’s central region called macula. Currently, there no treatment to replace Addressing this unmet need, we employed control isogenic hypoimmunogenic induced pluripotent stem cell (iPSC) lines generate spontaneously polarized retinal sheets (RSs). They presented advantage facile customization large-scale production, exhibiting...
Purpose We showed that compared to adult rats (AR), the retina of neonatal (NR) is significantly more resistant light damage. The purpose this study was determine minimum exposure time requires cause an adult-like retinopathy at long term. Methods Starting postnatal age 14 days (P14), NR were exposed 10,000 lux for various lengths [1, 2, 3, 6, 9, 12 and 16 consecutive (d)]. ERGs retinal histology performed predetermined points. Results Maximal effect obtained after 3 [50% control ONL...