A5011086162- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Genetics, Aging, and Longevity in Model Organisms
- Photosynthetic Processes and Mechanisms
- Erythrocyte Function and Pathophysiology
- Calcium signaling and nucleotide metabolism
- Metabolism, Diabetes, and Cancer
- Hippo pathway signaling and YAP/TAZ
- Neurogenesis and neuroplasticity mechanisms
- Protist diversity and phylogeny
- Signaling Pathways in Disease
- Cancer-related Molecular Pathways
- Neuroscience and Neural Engineering
- Pancreatic function and diabetes
- 14-3-3 protein interactions
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Chemokine receptors and signaling
- Medical Education and Admissions
- Innovative Teaching Methods
- Problem and Project Based Learning
- Axon Guidance and Neuronal Signaling
Université de Montréal
2014-2025
Centre Hospitalier Universitaire Sainte-Justine
2015-2025
University of California, San Francisco
2003-2008
University of Glasgow
1999-2005
Université Toulouse III - Paul Sabatier
1997
Cytokinesis requires a dramatic remodeling of the cortical cytoskeleton as well membrane addition. The Drosophila pericentrosomal protein, Nuclear-fallout (Nuf), provides link between these two processes. In nuf-derived embryos, actin and recruitment during initial stages metaphase cellular furrow formation are disrupted. Nuf is homologue arfophilin-2, an ADP ribosylation factor effector that binds Rab11 influences recycling endosome (RE) organization. Here, we show important component RE,...
Anillin is a conserved protein required for cytokinesis but its molecular function unclear. accumulation at the cleavage furrow Rho guanine nucleotide exchange factor (GEF)(Pbl)-dependent may also be mediated by known anillin interactions with F-actin and myosin II, which are under RhoGEF(Pbl)-dependent control themselves. Microscopy of Drosophila melanogaster S2 cells reveal here that although II do contribute, equatorial localization persists in their absence. Using latrunculin A,...
Arfophilin is an ADP ribosylation factor (Arf) binding protein of unknown function. It identical to the Rab11 eferin/Rab11-FIP3, and we show it binds both Arf5 Rab11. We describe a related protein, arfophilin-2, that interacts with in nucleotide-dependent manner, but not Arf1, 4, or 6 also Arfophilin-2 localized perinuclear compartment, centrosomal area, focal adhesions. The localization arfophilin-2 compartment was selectively blocked by overexpression Arf5-T31N. In contrast, green...
During cytokinesis, closure of the actomyosin contractile ring (CR) is coupled to formation a midbody (MR), through poorly understood mechanisms. Using time-lapse microscopy Drosophila melanogaster S2 cells, we show that transition from CR MR proceeds via previously uncharacterized maturation process requires opposing mechanisms removal and retention scaffold protein Anillin. The septin cytoskeleton acts on C terminus Anillin locally trim away excess membrane late CR/nascent internalization,...
Animal cell cytokinesis requires activation of the GTPase RhoA (Rho1 in Drosophila), which assembles an F-actin- and myosin II-dependent contractile ring (CR) at equatorial plasma membrane. CR closure is poorly understood, but involves multidomain scaffold protein, Anillin. Anillin binds many components including F-actin II (collectively actomyosin), septins. recruits septins to mechanism unclear. Live imaging Drosophila S2 cells HeLa revealed that N-terminus, scaffolds actomyosin, cannot...
Insulin and guanosine-5′-O-(3-thiotriphosphate) (GTPγS) both stimulate glucose transport translocation of the insulin-responsive transporter 4 (GLUT4) to plasma membrane in adipocytes. Previous studies suggest that these effects may be mediated by different mechanisms. In this study we have tested hypothesis agonists recruit GLUT4 distinct trafficking mechanisms, possibly involving mobilization intracellular compartments. We show ablation endosomal system using transferrin-HRP causes a...
Cell division requires the coordination of critical protein kinases and phosphatases. Greatwall (Gwl) kinase activity inactivates PP2A-B55 at mitotic entry to promote phosphorylation cyclin B–Cdk1 substrates, but how Gwl is regulated poorly understood. We found that subcellular localization changed dramatically during cell cycle in Drosophila. translocated from nucleus cytoplasm prophase. identified two nuclear signals central, characterized region Gwl, which are required for its function....
Drosophila melanogaster Polo and its human orthologue Polo-like kinase 1 fulfill essential roles during cell division. Members of the (Plk) family contain an N-terminal domain (KD) a C-terminal Polo-Box (PBD), which mediates protein interactions. How Plks are regulated in cytokinesis is poorly understood. Here we show that phosphorylation by Aurora B required for cytokinesis. This activation loop KD promotes dissociation from PBD-bound microtubule-associated Map205, acts as allosteric...
Cytokinesis is the last step of cell division that partitions cellular organelles and cytoplasm one into two. In animal cells, cytokinesis requires Rho-GTPase-dependent assembly F-actin myosin II (actomyosin) to form an equatorial contractile ring (CR) bisects cell. Despite 50 years research, precise mechanisms CR assembly, tension generation closure remain elusive. This hypothesis article considers a holistic view that, in addition actomyosin, includes another Rho-dependent cytoskeletal...
During animal cell cytokinesis, active RhoA assembles actomyosin-based contractile rings that tend to close asymmetrically. Through imaging C. elegans zygotes, Lebedev et al. (https://doi.org/10.1083/jcb.202405182) reveal the scaffold protein, anillin, promotes asymmetric ring closure by locally sequestering from its canonical effectors and thereby limiting actomyosin contractility.
The most common rearrangement in childhood precursor B-cell acute lymphoblastic leukemia is the t(12;21)(p13;q22) translocation resulting ETV6-AML1 fusion gene. A frequent concomitant event loss of residual ETV6 allele suggesting a critical role for transcriptional repressor etiology this cancer. However, precise mechanism through which functional contributes to disease pathogenesis still unclear. To investigate impact on network and identify new targets ETV6, we used whole transcriptome...
ADP-ribosylation factors (ARFs) play important roles in both constitutive and regulated membrane trafficking to the plasma other cells. Here we have examined their role insulin-stimulated GLUT4 translocation 3T3-L1 adipocytes. These cells express ARF5 ARF6. was identified soluble protein intracellular membranes; response insulin some observed re-locate membrane. In contrast, ARF6 predominantly localized did not redistribute insulin. We employed myristoylated peptides corresponding NH2...
Insulin increases the exocytosis of many soluble and membrane proteins in adipocytes. This may reflect a general effect insulin on protein export from trans Golgi network. To test this hypothesis, we have compared trafficking secreted serine protease adipsin integral GLUT4 transferrin receptors 3T3‐L1 We show that is network to endosomal system, as ablation endosomes using transferrin‐HRP conjugates strongly inhibited secretion. Phospholipase D has been implicated network, stimulates...