A5100612097- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Nuclear Structure and Function
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Ion Transport and Channel Regulation
- Face and Expression Recognition
- Plant biochemistry and biosynthesis
- Nanomaterials for catalytic reactions
- Photochemistry and Electron Transfer Studies
- Antibiotics Pharmacokinetics and Efficacy
- Protein Interaction Studies and Fluorescence Analysis
- Angiogenesis and VEGF in Cancer
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- Drug Transport and Resistance Mechanisms
- Calpain Protease Function and Regulation
- Biochemical and biochemical processes
- Histone Deacetylase Inhibitors Research
- Erythropoietin and Anemia Treatment
- Genetic Neurodegenerative Diseases
- Sodium Intake and Health
- Magnetic and Electromagnetic Effects
- Imbalanced Data Classification Techniques
South China Agricultural University
2025
Ministry of Agriculture and Rural Affairs
2025
Guangxi University
2024-2025
Fudan University
2009-2025
First Affiliated Hospital of Zhengzhou University
2025
Tianjin University of Commerce
2025
Nanjing Agricultural University
2023-2024
Shandong University
2023
Yangzhou University
2017-2023
Shanxi Agricultural University
2015-2021
Kinetics and cryo-electronmicroscopy data provide insights into GTPase ObgE's role as a ribosome anti-association factor that is modulated by nutrient availability, coupling growth control to biosynthesis protein translation.
Abstract Members of the solute carrier organic anion transporting polypeptide (OATPs) family function as transporters for a large variety amphipathic anions including endogenous metabolites and clinical drugs, such bile salts, steroids, thyroid hormones, statins, antibiotics, antivirals, anticancer drugs. OATP1B1 plays vital role in substances into liver hepatic clearance. FDA EMA recommend conducting vitro testing drug–drug interactions (DDIs) involving OATP1B1. However, structure working...
Background Heart failure (HF) guidelines recommend routine testing for iron deficiency (ID) and, those with ID, intravenous if the left ventricular ejection fraction is <50%. Guideline adherence to these recommendations by cardiologists in China unknown. Methods and results An independent academic web-based survey was designed distributed via social networks across China. Overall, 1342 (median age 34 years, IQR 30–39, 51% women) from all provinces of completed this survey. More than half...
Abstract PKD2L1, also termed TRPP3 from the TRPP subfamily (polycystic TRP channels), is involved in sour sensation and other pH-dependent processes. PKD2L1 believed to be a nonselective cation channel that can regulated by voltage, protons, calcium. Despite its considerable importance, molecular mechanisms underlying regulations are largely unknown. Here, we determine atomic structure at 3.38 Å resolution cryo-electron microscopy, whereby side chains of nearly all residues assigned. Unlike...
The nuclear pore complex (NPC) mediates the flow of substances between nucleus and cytoplasm in eukaryotic cells. Here we report cryo-electron tomography (cryo-ET) structure luminal ring (LR) NPC from Xenopus laevis oocyte. observed key structural features LR are independently confirmed by single-particle microscopy (cryo-EM) analysis. comprises eight butterfly-shaped subunits, each containing two symmetric wings. Each wing consists four elongated, tubular protomers. Within subunit,...
Abstract The nuclear pore complex (NPC) exhibits structural plasticity and has only been characterized at local resolutions of up to 15 Å for the cytoplasmic ring (CR). Here we present a single-particle cryo-electron microscopy (cryo-EM) structure CR from Xenopus laevis NPC average 5.5–7.9 Å, with reaching 4.5 Å. Improved allow identification placement secondary elements in majority components. two Y complexes each subunit interact other associate those flanking subunits, forming circular...
Background/Aims: Baicalin has been shown to be effective for various animal models of cardiovascular diseases, such as pulmonary hypertension, atherosclerosis and myocardial ischaemic injury. However, whether baicalin plays a role in cardiac hypertrophy remains unknown. Here we investigated the protective effects on induced by pressure overload explored potential mechanisms involved. Methods: C57BL/6J-mice were treated with or vehicle following transverse aortic constriction Sham surgery up...
Many ribosome-interacting GTPases, with proposed functions in ribosome biogenesis, are also implicated the cellular regulatory coupling between assembly process and various growth control pathways. EngA is an essential GTPase bacteria, intriguingly, it contains two consecutive domains (GD), being one-of-a-kind among all known GTPases. required for 50S subunit maturation. However, its molecular role remains elusive. Here, we present structure of bound to subunit. Our data show that binds...
The sodium-chloride cotransporter NCC mediates the coupled import of sodium and chloride across plasma membrane, playing vital roles in kidney extracellular fluid volume blood pressure control. Here, we present full-length structure human NCC, with 2.9 Å for transmembrane domain 3.8 carboxyl-terminal domain. adopts an inward-open conformation a domain-swap dimeric assembly. Conserved ion binding sites among cation-chloride cotransporters Na2 site are observed our structure. A unique His...
Flurtamone is a typical chiral pesticide with pair of enantiomers. In this study, the enantioselective biological effects flurtamone enantiomers were systematically investigated. The bioactivities R-flurtamone 6.3-35.6 and 1.7-9.9 times higher than S- Rac-flurtamone, respectively. contribution R-enantiomer to herbicidal activity was 86.3-97.3%. carotenoid content in treatment group 1.31-2.14 lower that S-flurtamone group. Molecular docking found can form aromatic H-bonds phenylalanine (PHE)...
Fruits undergo a similar ripening process, yet they exhibit range of differences in color, taste, and shape, both across different species within the same species. How does this diversity arise? We uncovered conserved fruit process lychee which NAC transcription factor, LcNAC1, acts as master regulator. LcNAC1 regulates expression two terpene synthase genes, LcTPSa1 LcTPSa2, belong to gene cluster consisting four TPS genes. LcTPSa1-LcTPSa3 are responsible for catalyzing production farnesol,...
Exonic deletions disrupting the autism susceptibility candidate 2 ( AUTS2 ) gene have been demonstrated as causal variants leading to neurodevelopmental disorders (NDDs) such spectrum disorder (ASD) and developmental delay (DD). Here, we report on 830 kb de novo deletion at chromosome 7q11.22 in a 4‐year‐old male patient with ASD DD. This disrupts promoter region exon 1 of , potentially complete haploinsuffiency gene. In addition, discuss clinical presentation light previous studies...
Rapid progress in the field of nerve tissue engineering has opened up way for new therapeutic strategies spinal cord injury (SCI). Bone marrow-derived mesenchymal stem cells (MSCs) could be differentiated into neural lineages, which can used as a potential cell source repair. Schwann (SCs) have been reported to support structural and functional recovery SCI. In this study, we co-cultured neurotrophin-3 (NT-3) gene-modified SCs NT-3 receptor tyrosine protein kinase C (TrkC) MSCs...
Highly active subnano Pd clusters were synthesized using i-motif DNA as the template through characterization via ESI MS, DLS, XPS, UV–vis, and FTIR. It is indicated that Pd1–Pd5 are generated at a [Pd]/[base] ratio of 0.2, Pd8 to Pd9 0.5, large nanoparticles with size about 2.6 nm formed 2.0. The i-motif-stabilized Pd8–Pd9 show high catalytic activity toward reduction 4-nitrophenol relative rate constant value 2034 min–1 (mM Pd)−1. DFT calculations disclose unique structure consecutive...
Hyper activity of protooncogene c-Myc is one the hallmarks highly aggressive lymphomas. However, mechanism how subjected to activation and amplification still not well defined. In this study, we use gene knockout strategy show that targeted depletion a well-conserved microRNA locus miR-144/451 initiates tumorigenesis including B-lymphoma development in aged mice. This due, at least part, direct by loss expression hematopoietic cells. Moreover, oncoprotein inversely regulates directly binding...
Introduction: Luscan-Lumish syndrome (LLS) is currently recognized as a rarely-observed condition featured with overgrowth, macrocephaly, obesity, type I Chiari malformation, and linguistic retardation. So far, there have been only few LLS cases registered worldwide, but none of them reported from China. To acquire deeper understanding on the clinical genetic features this disease, Chinese boy caused by heterozygous variant in SETD2 gene was investigated present study. Methods: The patient...
Abstract Vesicular monoamine transporters (VMAT1/2) are responsible for loading and packaging neurotransmitters into synaptic vesicles, including serotonin (5-HT), dopamine (DA), norepinephrine, histamine. Dysregulation of VMAT2 within the central nervous system can lead to schizophrenia, mood disorders, Parkinson’s disease, due imbalances these neurotransmitters. Medications such as tetrabenazine (TBZ) valbenazine (VBZ) targetting approved treating chorea associated with Huntington’s...
BackgroundAngiogenesis improves reperfusion to the ischaemic tissue after vascular obstruction. The underlying molecular mechanisms of post-ischaemic angiogenesis are not clear. FAM3A belongs family with sequence similarity 3 (FAM3) genes, but its biological function in endothelial cells regards diseases is well understood.MethodsGain- and loss-of-function methods by adenovirus or associated-adenovirus (AAV) different models were applied investigate effects on angiogenesis. Endothelial was...