A5101619896- Genetic and Kidney Cyst Diseases
- Renal Diseases and Glomerulopathies
- Genetic Syndromes and Imprinting
- Chronic Kidney Disease and Diabetes
- Biomedical Research and Pathophysiology
- Renal and related cancers
- Dialysis and Renal Disease Management
- Hedgehog Signaling Pathway Studies
- Acute Kidney Injury Research
- Pediatric Urology and Nephrology Studies
- Blood Pressure and Hypertension Studies
- Epigenetics and DNA Methylation
- Cardiac Arrest and Resuscitation
- Polyamine Metabolism and Applications
- Phosphorus and nutrient management
- Hormonal Regulation and Hypertension
- Wnt/β-catenin signaling in development and cancer
- Pharmacology and Obesity Treatment
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Ion Transport and Channel Regulation
- Multiple Myeloma Research and Treatments
- Intracranial Aneurysms: Treatment and Complications
- Prostate Cancer Treatment and Research
- Kidney Stones and Urolithiasis Treatments
- Vascular Malformations and Hemangiomas
Second Military Medical University
2013-2022
Shanghai Changzheng Hospital
2015-2021
Chinese People's Liberation Army
2020
University of Maryland, Baltimore
2014
University of Rochester Medical Center
2013
Johns Hopkins Medicine
2007-2010
Johns Hopkins University
2007-2010
Shanghai First People's Hospital
2008
A complex implicated in kidney health Autosomal dominant polycystic disease (ADPKD) is a common genetic that can lead to failure. Mutations the proteins PKD1 and PKD2 are linked disease, but function of these remains unclear, both physiology disease. has been sensing chemical mechanical force stimuli, proposed be calcium ion channel. Su et al. show transmembrane regions form PKD1-PKD2 assembled 1:3 ratio. Their high-resolution cryo–electron microscopy structure confirms adopts transient...
Polycystin-1 (PC1) has an essential function in renal tubular morphogenesis and disruption of its causes cystogenesis human autosomal dominant polycystic kidney disease. We have previously shown that recombinant PC1 is cis-autoproteolytically cleaved at the G protein-coupled receptor proteolytic site domain. To investigate role cleavage vivo, we generated by gene targeting a Pkd1 knockin mouse (Pkd1(V/V)) expresses noncleavable PC1. The Pkd1(V/V) mice show hypomorphic phenotype,...
Abstract PKD2L1, also termed TRPP3 from the TRPP subfamily (polycystic TRP channels), is involved in sour sensation and other pH-dependent processes. PKD2L1 believed to be a nonselective cation channel that can regulated by voltage, protons, calcium. Despite its considerable importance, molecular mechanisms underlying regulations are largely unknown. Here, we determine atomic structure at 3.38 Å resolution cryo-electron microscopy, whereby side chains of nearly all residues assigned. Unlike...
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a genetic disorder resulting in large kidney cysts and eventual failure. Mutations either the PKD1 or PKD2/TRPP2 genes their respective protein products, polycystin-1 (PC1) polycystin-2 (PC2) result ADPKD. PC2 known to function as non-selective cation channel, but PC1's of PC1 cleavage products are not well understood. Here we identify an endogenous product, P100, 100 kDa fragment found both wild type epitope tagged knock-in mice....
Polycystin-1 (Pc1) cleavage at the G protein-coupled receptor (GPCR) proteolytic site (GPS) is required for normal kidney morphology in humans and mice. We found a complex pattern of endogenous Pc1 forms by GPS cleavage. generates not only heterodimeric cleaved full-length (Pc1(cFL)) which N-terminal fragment (NTF) remains noncovalently associated with C-terminal (CTF) but also novel form (Pc1(deN)) NTF becomes detached from CTF. Uncleaved (Pc1(U)) resides primarily endoplasmic reticulum...
The prostate cancer (PCa) microenvironment contains active stromal cells known as cancer-associated fibroblasts (CAF) that may play important roles in influencing tumor progression. Here we studied the role of CAF estrogen receptor alpha (ERα) and found it could protect against PCa invasion. Immunohistochemistry on prostatectomy specimens showed patients with ERα-positive stroma had a significantly lower risk for biochemical recurrence. In vitro invasion assays further confirmed ERα was able...
Emerging evidence indicates that epigenetic modulation of gene expression plays a key role in the progression autosomal dominant polycystic kidney disease (ADPKD). However, molecular basis for how altered epigenome modulates transcriptional responses, and thereby ADPKD, remains largely unknown.Kidneys from control ADPKD mice were examined CDYL histone acylations. its correlation with severity analyzed cohort patients ADPKD. Cdyl transgenic crossed Pkd1 knockout to explore CDYL's progression....
The PKD1 or PKD2 genes encode polycystins (PC) 1 and 2, which are associated with polycystic kidney disease. Previously we demonstrated that PC2 interacts the inositol 1,4,5-trisphosphate receptor (IP(3)R) to modulate Ca(2+) signaling. Here, investigate whether PC1 also regulates IP(3)R. We generated a fragment encoding last six transmembrane (TM) domains of C-terminal tail (QIF38), section highest homology PC2. Using Xenopus oocyte imaging system, observed expression QIF38 significantly...
Objective Autosomal dominant polycystic kidney disease (ADPKD) is a relentlessly progressing form of chronic for which there no cure. The aim this study was to characterize Chinese patients with ADPKD and identify the factors predict cyst growth renal functional deterioration. Methods To analyze predicting we performed prospective longitudinal observational in cohort 541 an eGFR ≥30 ml/min/1.73 m2. Patients were followed clinically radiologically sequential abdominal magnetic resonance...
Background The inpatient morbidity and mortality of acute kidney injury (AKI) vary considerably in different clinical units, yet studies to compare the difference remain limited. Methods We compared characteristics AKI Intensive Care Unit (ICU), medical surgical departments by using data derived from 2013 nationwide cross-sectional survey China capture variations among recognition, management, outcomes AKI. Suspected patients were identified based on changes serum creatinine during...
Post-translational degradation of protein plays an important role in cell life.We employed chimeric molecules (dihydrotestosterone-based proteolysis-targeting molecule [DHT-PROTAC]) to facilitate androgen receptor (AR) via the ubiquitin-proteasome pathway (UPP) and investigate AR proliferation viability androgen-sensitive prostate cancer cells.Western blot analysis immunohistochemistry were applied analyse levels LNCaP cells after DHT-PROTAC treatment.Cell counting...
Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary disease, with a prevalence of 1/2,500–1/1,000, and it affects 1.25 million people in China. ADPKD responsible for nearly 5% end-stage renal cases, which leads to major burden on public health. In 2016, Chinese working group developed guidelines diagnosis treatment ADPKD, promoted clinical management last 3 years, clinicians have deepened their understanding standardized several basic studies been conducted....
To investigate the clinical significance of E-cadherin (E-CD) expression in human bladder transitional cell carcinoma (TCC) tissue and soluble forms (sE-CD) urine patients with TCC.One hundred two specimens TCC 10 normal tissues were stained immunohistochemically anti-E-CD monoclonal antibody. The urinary sE-CD from 59 subjects or controls was measured enzyme-linked immunosorbent assay (ELISA). All results analyzed statistically between groups.The E-CD correlated well grade stage but had no...
Autosomal dominant polycystic kidney disease (ADPKD) is a complex process, involving the alteration of multiple genes and signaling pathways, pathogenesis ADPKD remains largely unknown. Here, we demonstrated suppressive role sorting nexin 9 (SNX9) during development. SNX9 expression was detected in tissues patients, for first time, also Pkd1 knockout (Pkd1-/-) control mice. Subsequently, series gain- loss-of-function studies were performed, to explore biological roles underlying molecular...
Introduction Co-administration of a diuretic or calcium channel blocker with an ACE inhibitor are both preferred combinations in patients hypertensive chronic kidney disease (CKD). According to the available evidence, it is still unknown which combination plays more active role renal protection. We hypothesised that fosinopril and benidipine may delay progression CKD effectively than hydrochlorothiazide (HCTZ). Methods analysis This study will be multicentred, prospective, double-blind,...
<b><i>Background/Aims:</i></b> A lack of baseline serum creatinine (SCr) data leads to underestimation the burden caused by acute kidney injury (AKI) in developing countries. The goal this study was investigate effects various SCr analysis methods on current diagnosis AKI hospitalized patients. <b><i>Methods:</i></b> Patients with at least one value during their hospital stay between January 1, 2011 and December 31, 2012 were retrospectively...