A5037644867- Nerve injury and regeneration
- Spinal Cord Injury Research
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Nerve Injury and Rehabilitation
- Pluripotent Stem Cells Research
- Microbial Fuel Cells and Bioremediation
- Tissue Engineering and Regenerative Medicine
- Glioma Diagnosis and Treatment
- Geological and Geophysical Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Wound Healing and Treatments
- Protist diversity and phylogeny
- Semiconductor Lasers and Optical Devices
- Transcranial Magnetic Stimulation Studies
- Hepatitis C virus research
- Conducting polymers and applications
- Signaling Pathways in Disease
- Electrospun Nanofibers in Biomedical Applications
- Anesthesia and Neurotoxicity Research
- Geological and Geochemical Analysis
- Hepatitis Viruses Studies and Epidemiology
Guangzhou University of Chinese Medicine
2022-2025
Sun Yat-sen University
2016-2025
Spaulding Rehabilitation Hospital
2015-2024
Brigham and Women's Hospital
2015-2024
Harvard University
2015-2024
Nantong University
2023-2024
Zhejiang Chinese Medical University
2024
Mass General Brigham
2023-2024
The University of Tokyo
2024
Chinese Center For Disease Control and Prevention
2024
Three-dimensional (3D) gelatin sponge (GS) scaffolds were constructed by ensheathing GS with a thin film of poly-(lactide- co-glycolide) (PLGA). Rat bone marrow-derived mesenchymal stem cells (MSCs) isolated, cultured, and then seeded to the scaffolds. Distribution cell growth, survival, proliferation within determined. Immunofluorescence Western blot analysis employed detect deposition fibronectin on day 3 7 culture. Scaffolds or without MSCs transplanted into transected rat spinal cord....
Two Shewanella -like bacterial strains, WP2 T and WP3 , which were isolated from west Pacific deep-sea sediment, studied to determine their taxonomic position. Cells of the two bacteria facultatively anaerobic, Gram-negative rods motile by means a single polar flagellum. Strain was psychrophilic, growing optimally at about 10–15 °C, whereas strain psychrotolerant, 15–20 °C. The strains grew in pressure range 0.1–50 MPa, with optimal growth 20 MPa. able use nitrate, fumarate, trimethylamine N...
An obligate piezophilic anaerobic hyperthermophilic archaeon, designated strain CH1(T), was isolated from a hydrothermal vent site named 'Ashadze', which is located on the Mid-Atlantic Ridge at depth of 4100 m. Enrichment and isolation were carried out 95 °C under hydrostatic pressure 42 MPa. Cells CH1(T) highly motile irregular cocci with diameter ~1-1.5 µm. Growth recorded 80-108 (optimum 98 °C) pressures 20-120 MPa 52 MPa). No growth observed atmospheric 60-110 °C. pH 6.0-9.5 7.5-8.0) in...
Significance We developed a platform technology to determine therapeutic mechanisms of human mesenchymal stromal stem cells (hMSCs) in dorsal root ganglion coculture system and an intraspinal cord implantation model. The unique poly(lactic- co- glycolic) acid scaffolding augments hMSC stemness, engraftment, function without neural transdifferentiation or lineage development, resulting robust motosensory improvement, pain tissue damage mitigation, myelin preservation adult rat spinal after...
A novel sulfate-reducing bacterium, strain J2T, was isolated from a serpentinized peridotite sample the Indian Ocean. Phylogenetic analysis based on 16S rRNA gene sequences showed that J2T clustered with genus Desulfovibrio within family Desulfovibrionaceae, but it low similarity (87.95 %) to type species desulfuricans DSM 642T. It most closely related portus MSL79T (96.96 %), followed by aespoeensis Aspo-2T (96.11 piezophilus C1TLV30T (96.04 and profundus 11384T (95.17 %). Other available...
ABSTRACT Sulfur is an important element in sustaining microbial communities present hydrothermal vents. oxidation has been extensively studied due to its importance chemosynthetic pathways fields; however, less known about sulfate reduction. Here, the metagenomes of chimneys located on ultraslow-spreading Southwest Indian Ridge (SWIR) were pyrosequenced elucidate associated sulfur cycle. A taxonomic summary genes revealed a few dominant bacteria that participated cycle, particularly...
We have reported previously that bone marrow mesenchymal stem cell (MSC)-derived neural network scaffold not only survived in the injury/graft site of spinal cord but also served as a "neuronal relay" was capable improving limb motor function complete injury (SCI) rat model. It remained to be explored whether such strategy effective for repairing large tissue loss well restoring larger animals. therefore extended this study construct canine MSC-derived vitro with aim evaluate its efficacy...
The mechanism underlying neurogenesis during embryonic spinal cord development involves a specific ligand/receptor interaction, which may be help guide neuroengineering to boost stem cell-based neural regeneration for the structural and functional repair of injury. Herein, we hypothesized that supplying defects with an exogenous network in NT-3/fibroin-coated gelatin sponge (NF-GS) scaffold might improve tissue efficacy. To test this, engineered tropomyosin receptor kinase C (TrkC)-modified...
Neuromuscular diseases usually manifest as abnormalities involving motor neurons, neuromuscular junctions, and skeletal muscle (SkM) in postnatal stage. Present vitro models of interactions require a long time lack neuroglia involvement. Our study aimed to construct rodent bioengineered spinal cord neural network-skeletal (NN-SkM) assembloids elucidate the between stem cells (SC-NSCs) SkM their biological effects on development maturation circuits. After coculture with cells, SC-NSCs...
We previously reported that the ethanol extract of <i>Schisandra sphenanthera</i> [Wuzhi (WZ) tablet] significantly protects against acetaminophen-induced hepatoxicity. However, whether WZ exerts a protective effect cholestasis remains unclear. In this study, on lithocholic acid (LCA)–induced intrahepatic in mice was characterized and involved mechanisms were investigated. pretreatment (350 mg/kg) with LCA reversed liver necrosis decreased serum alanine aminotransferase, aspartate alkaline...
Abstract Tissue engineering–based neural construction holds promise in providing organoids with defined differentiation and therapeutic potentials. Here, a bioengineered transplantable spinal cord–like tissue (SCLT) is assembled vitro by simulating the white matter gray composition of cord using stem cell–based engineering technique. Whether organoid would execute targeted repair injured evaluated. The integrated SCLT, matter–like (WMLT) module (GMLT) module, shares architectural,...
After spinal cord injury (SCI), neutrophil elastase (NE) released at site disrupts vascular endothelium integrity and stabilization. Angiopoietins (ANGPTs) are growth factors that play an important role in We hypothesized is one of the key determinants disruption/destabilization affects angiopoietins expression after injury. To test this, tubule formation were assessed endothelial cells exposed to different concentrations recombinant neutropil elastase. Then, angiopoietin-1, angiopoietin-2,...
Edema in the central nervous system can rapidly result life‐threatening complications. Vasogenic edema is clinically manageable, but there no established medical treatment for cytotoxic edema, which affects astrocytes and a primary trigger of acute post‐traumatic neuronal death. To test hypothesis that adrenergic receptor agonists, including stress stimulus epinephrine protects neural parenchyma from damage, we characterized its effects on hypotonicity‐induced cellular cortical by vivo vitro...