A5103131096- Soybean genetics and cultivation
- Agricultural pest management studies
- Genetics and Plant Breeding
- Legume Nitrogen Fixing Symbiosis
- Bone and Joint Diseases
- Neurotransmitter Receptor Influence on Behavior
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Hip disorders and treatments
- Neuroscience and Neuropharmacology Research
- Bone health and treatments
- Receptor Mechanisms and Signaling
- Bone fractures and treatments
- Endoplasmic Reticulum Stress and Disease
- Neuropeptides and Animal Physiology
- Nerve injury and regeneration
- Peptidase Inhibition and Analysis
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Sulfur Compounds in Biology
- Adipokines, Inflammation, and Metabolic Diseases
- Genomics and Phylogenetic Studies
- Cancer-related gene regulation
- PARP inhibition in cancer therapy
Jiangsu Academy of Agricultural Sciences
2020-2025
Union Hospital
2016-2024
Huazhong University of Science and Technology
2008-2024
Chinese PLA General Hospital
2012-2024
Northwest A&F University
2020-2024
Union Hospital
2017-2024
Nantong University
2022-2023
Xi'an Medical University
2021-2022
Shanghai Ocean University
2022
Nanjing Agricultural University
2021-2022
Aims: Hydrogen sulfide (H2S) has a protective role in the pathogenesis of atherosclerosis by multiple pathways. Sirtuin-1 (SIRT1) is histone deacetylase, as an essential mediated longevity gene, and anti-atherogenic effect regulating acetylation some functional proteins. Whether SIRT1 involved protecting H2S its mechanism remains unclear. Results: In ApoE-knockout mice, treatment with donor (NaHS or GYY4137) reduced atherosclerotic plaque area, macrophage infiltration, aortic inflammation,...
Abstract Vascular calcification is highly prevalent in end-stage renal diseases and predictive of cardiovascular events mortality. Poly(ADP-ribose) polymerase 1 (PARP1) inhibition or deletion vasoprotective several disease models. Here we show that PARP activity increased radial artery samples from patients with chronic failure, arteries uraemic rats, calcified vascular smooth muscle cells (VSMCs) vitro. PARP1 deficiency blocks, whereas overexpression exacerbates, the transdifferentiation...
Abstract FUN14 domain-containing protein 1 (FUNDC1) is an integral mitochondrial outer-membrane protein, and mediates the formation of mitochondria-associated endoplasmic reticulum membranes (MAMs). This study aims to determine contributions FUNDC1-mediated MAMs angiogenesis in vitro vivo. In cultured endothelial cells, VEGF significantly increases MAM-related proteins, including FUNDC1. Endothelial cell-specific deletion FUNDC1, which disrupts MAM lowers VEGFR2 expression reduces tube...
Hydrogen sulfide (H2 S) plays a protective role in NAFLD. However, whether cystathionine γ lyase (CSE), dominant H2 S generating enzyme hepatocytes, has the pathogenesis of NAFLD is currently unclear.We showed that CSE protein expression dramatically downregulated, especially fibrotic areas, livers from patients with In high-fat diet (HFD)-induced mice or an oleic acid-induced hepatocyte model, CSE/H2 pathway also downregulated. To illustrate regulatory for NAFLD, we generated...
Autophagy is a key regulatory process in maintaining cellular homoeostasis via lysosome degradation. Growing evidence reveals that poly(ADP-ribose) polymerase-1 (PARP1) involved the progression of many cardiovascular diseases. This study was undertaken to discuss role PARP1 cardiomyocyte autophagy. Our results demonstrated activated response starvation-induced myocardial We identified Forkhead box O (FoxO)3a as substrate PARP1. Upon activation, poly(ADP-ribosyl)ation dissociated histone H1...
Single-cell RNA sequencing (scRNA-seq) is useful for exploring cell heterogeneity. For large animals, however, little known regarding spermatogonial stem (SSC) self-renewal regulation, especially in dairy goats. In this study, we described a high-resolution scRNA-seq atlas derived from goat. We identified six somatic and five spermatogenic subtypes. During spermatogenesis, genes with significantly changed expression were mainly enriched the Notch, TGF-β, Hippo signaling pathways as well...
Abstract Aims The heart undergoes pathological remodelling, featured by the hypertrophic growth of cardiomyocytes and increased cardiac fibrosis, under biomechanical stress such as haemodynamic overload. Ring Finger Protein 207 (RNF207) is an E3 ubiquitin ligase that predominantly expressed in heart, but its function remains elusive. In this study, we aimed to explore role RNF207 development hypertrophy dysfunction. Methods results Transverse aortic constriction (TAC) surgery was performed...
Bone marrow is an important source of stem cells, which can promote bone fracture healing. We investigated the optimal time to inject mesenchymal cells (BMSCs) in a C57 murine unilateral, transverse, femur model. BMSCs transfected with red fluorescent protein (RFP-BMSCs) were injected via tail vein on day 1, 7, or 14 post-fracture. AMD3100 (inhibitor stromal cell-derived factor 1 [SDF-1]) was also before RFP-BMSCs one group for comparison; control received saline injections. RFP-BMSC...
Methamphetamine (METH) is a psychostimulant that induces excessive release of dopamine (DA) in the striatum. In this study we have assessed role DA D1 and D2 receptors (D1R D2R) on striatal METH-induced apoptosis depletion DA-terminal markers. Male mice were given one i.p. injection METH (30 mg/kg). Apoptosis was at 24 h, marker 3 days, after METH. A single toxic dose induced approximately 10–13% neurons. This completely prevented by pretreatment min before METH) with either D1R antagonist...
C1q/tumor necrosis factor-related protein 13 (CTRP13) is a secreted adipokine that can ameliorate abnormal glucose and lipid metabolism. However, the functional role of CTRP13 in development atherosclerotic plaques has yet to be described. In this study, we collected blood samples from patients coronary artery diseases apolipoprotein E (ApoE)-/- mice were fed Western diet for 12 wk induce atherosclerosis found serum level was decreased. En face staining aortas aortic sinus ApoE-/- showed...
Abstract Orchitis is one of the leading causes male animal infertility and associated with inflammatory reactions caused by bacterium. It has been reported that there a mutual coupling effect between endoplasmic reticulum stress (ERS) response. Our studies showed lipopolysaccharide (LPS) could cause testicular damages, apoptosis, ERS, responses in spermatogonial stem cells (SSCs); ERS‐related apoptosis proteins were activated expression ERS genes was significantly upregulated; meanwhile,...
Despite pluripotent stem cells sharing key transcription factors, their maintenance involves distinct genetic inputs. Emerging evidence suggests that super-enhancers (SEs) can function as master regulatory hubs to control cell identity and pluripotency in humans mice. However, whether pluripotency-associated SEs share an evolutionary origin mammals remains elusive. Here, we performed comprehensive comparative epigenomic factor binding analyses among pigs, humans, mice identify SEs. Like...
Soybean (Glycine max [L.] Merr.) is one of the primary sources plant protein and oil for human foods, animal feed, industrial processing. The seed number per pod generally varies from to four an important component unit area yield. We used natural variation in 264 landraces improved cultivars or lines identify candidate genes involved regulation soybean. Genome-wide association tests revealed 65 loci that are associated with trait. Among them, 11 could be detected multiple environments....
Abstract Hyperhomocysteinaemia (HHcy)‐impaired endothelial dysfunction including endoplasmic reticulum (ER) stress plays a crucial role in atherogenesis. Hydrogen sulphide (H 2 S), metabolic production of Hcy and gasotransmitter, exhibits preventing cardiovascular damages induced by HHcy reducing ER stress, but the underlying mechanism is unclear. Here, we made an atherosclerosis with mice model ApoE knockout feeding Pagien diet drinking L‐methionine water. H S donors NaHS GYY4137 treatment...
Total soluble sugar (TSS) is an important component in vegetable soybean seeds during the R6 stage and greatly impacts fresh flavor. Increasing TSS content thus one of most breeding objectives for creation high-quality germplasm. To better understand genetic basis at stage, we investigated 264 germplasm accessions two environments. We obtained five associations with 27 significant SNPs using GWAS. The S15_10810881–S15_10843821 S06_12044239–S06_12048607 were identified both then conducted...
Abstract Acetaminophen (APAP) overdose is the most frequent cause of acute liver failure and remains a critical problem in medicine. PARP1-dependent poly(ADPribosyl)ation key mediator cellular stress responses functions multiple physiological pathological processes. However, whether it involved process APAP metabolism elusive. In this study, we find that PARP1 activated mouse livers after overdose. Pharmacological or genetic manipulations are sufficient to suppress APAP-induced hepatic...