A5100320548- Soybean genetics and cultivation
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Plant Molecular Biology Research
- Agricultural pest management studies
- Plant nutrient uptake and metabolism
- Genetics and Plant Breeding
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Biofuel production and bioconversion
- Machine Learning in Bioinformatics
- Genomics and Phylogenetic Studies
- Plant responses to water stress
- Phytase and its Applications
- Enzyme Production and Characterization
- Transgenic Plants and Applications
- Polysaccharides and Plant Cell Walls
- Plant Genetic and Mutation Studies
- Aluminum toxicity and tolerance in plants and animals
- GABA and Rice Research
- RNA and protein synthesis mechanisms
- Plant Parasitism and Resistance
Chinese Academy of Agricultural Sciences
2016-2025
Biotechnology Research Institute
2016-2025
Jiangsu Academy of Agricultural Sciences
2014-2025
Nanjing Agricultural University
2010-2025
Hunan Normal University
2025
China Tobacco
2025
Tobacco Research Institute
2025
Hunan University
2019-2024
Bohai University
2022-2024
Hebei Agricultural University
2020-2024
The acquisition of a thermostable enzyme is an indispensable prerequisite for its successful implementation in industrial applications and the development novel functionalities. Various protein engineering approaches, including rational design, semirational directed evolution, have been employed to enhance thermostability. However, all these approaches require sensitive reliable high-throughput screening (HTS) technologies efficiently rapidly identify variants with improved properties. While...
Abstract Background Yellow Stripe-Like (YSL) proteins are involved in the uptake and transport of metal ions. They play important roles maintaining zinc iron homeostasis Arabidopsis , rice ( Oryza sativa ), barley Hordeum vulgare ). However, this family have not been fully identified comprehensively analyzed maize Zea mays L.). Results In study, we 19 ZmYSL s genome their structural features. The results a phylogenetic analysis showed that ZmYSLs homologous to YSLs rice, these divided into...
SWEET (Sugar Will Eventually be Exported Transporter) proteins play vital roles in the transport of sugars, contributing to regulation plant development, hormone signaling, and responses abiotic stress. In this study, we identified 57 NtSWEETs tobacco (Nicotiana tabacum L.), then their physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter cis-elements, protein interaction network, tissue specificity, expression pattern were...
Summary Genetically modified crops have profound impacts on cost savings and environmental friendliness conferred by new traits, such as resistance to insects herbicides. Selectable marker genes are essential for screening transformed cells, but they undesirable in the final product due risks of horizontal gene transfer extensive safety assessment requirements. Generating marker‐ backbone‐free lines can enhance public acceptance transgenic crops. Here, we established a transposon‐mediated...
ABSTRACT Mothers against dpp (Mad) mediates Decapentaplegic (DPP) signaling throughout Drosophila development. Here we demonstrate that Medea encodes a MAD-related protein functions in DPP signaling. MEDEA is most similar to mammalian Smad4 and forms heteromeric complexes with MAD. Like dpp, essential for embryonic dorsal/ventral patterning. However, Mad the germline oogenesis whereas dispensable. In wing primordium, loss of severely affects regions receiving low signal. localized cytoplasm,...
Abstract A promoter that enabled high-level expression of the target gene during stationary phase in absence an inducer would facilitate efficient production heterogeneous proteins at a low cost. In this study, genome-scale microarray-based approach was employed to identify promoters induced genes Bacillus subtilis from late log without inducer. Eleven candidate were selected based on B. microarray data and quantitative PCR analysis. Among promoters, P ylb exhibited highest activity with...
Microorganisms play an important role in the remediation of cadmium pollution soil and their diversity can be affected by cadmium. In this study, bacterial community arable samples collected from two near geographical sites, with different degrees at three seasons, were characterized using Illumina MiSeq sequencing. The result showed that is factor to affect microbial communities high polluted area (the site H) had significant differences compared low L). Especially, higher concentrations Cd...
The effects of fermentation with different combinations probiotic micro-organisms on the consumer quality attributes, soy isoflavone and equol content yogurt, made from whey, embryo powder protein were investigated. Differences in water holding capacity, texture, apparent viscosity, sensory studied. Different probiotics had significantly yogurt quality, content. Fermentation Danisco starter resulted shortest time, best textural properties, flavor, scores, highest total (28) aroma volatiles...
The AP2/ERF gene family is one of the most conserved and important transcription factor families mainly occurring in plants with various functions regulating plant biological physiological processes. However, little comprehensive research has been conducted on Rhododendron (specifically, simsii), an ornamental plant. existing whole-genome sequence provided data to investigate genes a genome-wide scale. A total 120 were identified. phylogenetic analysis showed that RsAP2 classified into five...
Leaf senescence reduces the photosynthetic capacity of leaves, thus significantly affecting growth, development, and yield formation cotton. Melatonin (MT) is a multipotent substance proven to delay leaf senescence. However, its potential mechanism in delaying induced by abiotic stress remains unclear. This study aimed explore effect MT on drought-induced cotton seedlings clarify morphological physiological mechanisms. Drought upregulated marker genes, destroyed photosystem, led excessive...
RPGR-interacting protein-1 (RPGRIP1) is localized in the photoreceptor-connecting cilium, where it anchors RPGR (retinitis pigmentosa GTPase regulator) protein, and its function essential for photoreceptor maintenance. Genetic defect RPGRIP1 a known cause of Leber congenital amaurosis (LCA), severe, early-onset form retinal degeneration. We evaluated efficacy replacement gene therapy murine model LCA carrying targeted disruption RPGRIP1. The construct, packaged an adeno-associated virus...
Oxidative stress-induced retinal pigment epithelium (RPE) senescence is one of the important factors in pathogenesis age-related macular degeneration (AMD). This study aimed to develop a new antisenescence-based intervention and clarify its possible molecular mechanism.A cell premature model was established both primary RPE cells ARPE-19 by exposure pulsed H₂O₂ stress for 5 days, confirmed with senescence-associated β-galactosidase (SA-β-gal) staining. The final concentration fullerenol...
Iron and zinc are important micronutrients for both the growth nutrient availability of crop plants, their absorption is tightly controlled by a metal uptake system. Zinc-regulated transporters, iron-regulated transporter-like proteins (ZIP), considered an essential transporter acquisition Fe Zn in graminaceous plants. Several ZIPs have been identified maize, although physiological function remains unclear. In this report, ZmIRT1 was shown to be specifically expressed silk embryo, whereas...
Abstract Zinc (Zn) and iron (Fe) are essential micronutrients for plant growth. Thus, it is important to understand the mechanisms of uptake, transport accumulation these in maize improve crop nutritional quality. Members zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) family responsible uptake divalent metal ions plant. Previously, we showed that ZmZIP5 functionally complemented Zn double mutant zrt1zrt2, Fe-uptake fet3fet4 yeast. In our β-glucuronidase (GUS)...