A5019572378- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Tryptophan and brain disorders
- HIV Research and Treatment
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Prostate Cancer Treatment and Research
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Research and Treatments
- Melanoma and MAPK Pathways
- Iron Metabolism and Disorders
- Clostridium difficile and Clostridium perfringens research
- Hemoglobinopathies and Related Disorders
- Stress Responses and Cortisol
- Single-cell and spatial transcriptomics
- Trace Elements in Health
- Gut microbiota and health
- Cancer, Stress, Anesthesia, and Immune Response
Centre Hospitalier de l’Université de Montréal
2016-2025
Université de Montréal
2016-2025
Montreal Neurological Institute and Hospital
2025
Princess Margaret Cancer Centre
2018
McGill University Health Centre
2018
Université Libre de Bruxelles
2018
University of Toronto
2018
Institut Jules Bordet
2018
Tunis El Manar University
2016
Data Harbor (United States)
2015
Dendritic cells (DCs) play a key role in the activation and regulation of B T lymphocytes. Production indoleamine 2, 3-dioxygenase (IDO) by macrophages has recently been described to result inhibition cell proliferation through tryptophan degradation. Since DCs can be derived from monocytes, we sought determine whether could produce IDO which potentially regulate proliferation. Northern blot analysis RNA cultured monocyte-derived human DC revealed that mRNA was induced upon with CD40 ligand...
Understanding the tumor immune microenvironment (TIME) promises to be key for optimal cancer therapy, especially in triple-negative breast (TNBC). Integrating spatial resolution of cells with laser capture microdissection gene expression profiles, we defined distinct TIME stratification TNBC, implications current therapies including checkpoint blockade. TNBCs an immunoreactive exhibited tumoral infiltration granzyme B+CD8+ T (GzmB+CD8+ cells), a type 1 IFN signature, and elevated multiple...
Melanoma is an immunogenic cancer with a high response rate to immune checkpoint inhibitors (ICIs). It harbors mutation burden compared other cancers and, as result, has abundant tumor-infiltrating lymphocytes (TILs) within its microenvironment. However, understanding the complex interplay between stroma, tumor cells, and distinct TIL subsets remains substantial challenge in oncology. To properly study this interplay, quantifying spatial relationships of multiple cell types microenvironment...
A number of perturbations B cells has been described in the setting HIV infection; however, most remain poorly understood. To directly address effect replication on cell function, we investigated capacity isolated from HIV-infected patients to respond a variety stimuli before and after reduction viremia by effective antiretroviral therapy. taken with high levels plasma were defective their proliferative responses various stimuli. Viremia was also associated appearance subpopulation that...
In addition to new tumour antigens, prognostic and diagnostic markers are needed for common cancers. this study, we report the expression of Dickkopf-1 (DKK1) in multiple This constitutes a comprehensive analysis DKK1 profile. was evaluated by classical quantitative reverse transcriptase–polymerase chain reaction (RT–PCR) enzyme-linked immunosorbant assay protein determination, cancer lines clinical specimens several origins. For breast cancer, correlated with clinicopathological parameters....
The success of immunotherapy relies on the participation all arms immune system and role CD4+ T lymphocytes in preventing tumor growth is now well established. Understanding how tumors evade responses holds key to development cancer immunotherapies. In this review, we discuss MHC Class II expression varies cells influences antitumor responses. We also means that are currently available for harnessing antigen presentation pathway efficient vaccines activate against cancer.
Aneurysmal subarachnoid hemorrhage (SAH) is a catastrophic disease with devastating consequences, including high mortality rate and severe disabilities among survivors. Inflammation induced following SAH, but the exact role phenotype of innate immune cells remain poorly characterized. We investigated inflammatory components early brain injury in an animal model SAH patients.SAH was through injection blood space C57Bl/6 J wild-type mice. Prospective collections were obtained at 12 h, days 1,...
Abstract Immune checkpoint inhibitor (ICI) use remains a challenge in patients with solid organ allografts as most would undergo rejection. In melanoma patient whom programmed-death 1 (PD-1) blockade resulted rejection and colitis, the addition of mTOR sirolimus ongoing anti-tumor efficacy while promoting allograft tolerance. Strong granzyme B + , interferon (IFN)-γ CD8 cytotoxic T cell circulating regulatory (T reg ) responses were noted during rejection, along significant eosinophilia...
Vagal neurostimulation (VNS) is used for the treatment of epilepsy and major medical-refractory depression. VNS has neuropsychiatric functions systemic anti-inflammatory activity. The objective this study to measure clinical efficacy impact modulation in depressive patients. Six patients with refractory depression were enrolled. Depression symptoms assessed Montgomery-Asberg Rating, anxiety Hamilton Anxiety Rating Scale. Plasmas harvested prospectively before implantation (baseline) up 4...
Tryptophan (Trp) catabolism into immunosuppressive kynurenine (Kyn) by indoleamine 2,3-dioxygenase (IDO) was previously linked to Th17/Treg differentiation and immune activation. Here we examined Trp its impact on balance in uninfected healthy subjects (HS) a large cohort of HIV-infected patients with different clinical outcomes: ART-naïve, Successfully Treated (ST), elite controllers (EC). In ART-naïve patients, increased IDO activity/expression, together elevated levels TNF-α sCD40L, were...
It was originally thought that the critical role of CD40 ligand (CD40L) in normal and inflammatory immune responses mainly mediated through its interaction with classic receptor, CD40. However, data from CD40L(-/-) CD40(-/-) mice suggest CD40L-induced response involves at least one other receptor. This hypothesis is supported by fact CD40L stabilizes arterial thrombi an alphaIIbbeta3-dependent mechanism. Here we provide evidence soluble (sCD40L) binds to cells undifferentiated human...
Currently used vaccines protect mainly through the production of neutralizing antibodies. However, antibodies confer little or no protection for a majority chronic viral infections that require active involvement cytotoxic T lymphocytes (CTLs). Virus-like particles (VLPs) have been shown to be efficient inducers cell-mediated immune responses, but administration an adjuvant is generally required. We recently reported generation novel VLP system exploiting self-assembly property papaya mosaic...
Summary CD40/CD40-ligand (CD40L) signalling is a key stimulatory pathway which triggers the tryptophan (Trp) catabolizing enzyme IDO in dendritic cells and immunosuppressive cancer. We reported IDO-induced Trp catabolism results T helper type 17 (Th17)/regulatory cell (Treg) imbalance, favours microbial translocation HIV chronic infection. Here we assessed link between sCD40L, Tregs activity HIV-infected patients with different clinical outcomes. Plasmatic sCD40L inflammatory cytokines were...
Abstract Breast cancer diagnosed within 10 years following childbirth is defined as postpartum breast (PPBC) and highly metastatic. Interactions between immune cells other stromal the involuting mammary gland are fundamental in facilitating an aggressive tumor phenotype. The MNK1/2–eIF4E axis promotes translation of prometastatic mRNAs cells, but its role modulating function nontumor PPBC microenvironment has not been explored. Here, we used a combination vivo models vitro assays to study...
Highlights•The NF1 tumor suppressor binds the immune checkpoint protein PD-L1 in melanoma cell lines•NF1 loss increases surface expression•NF1 promotes resistance to T killing that is abrogated by anti-PD-1/PD-L1 blockade•NF1 regulation of independent its RAS-GAP activitySummaryHotspot BRAF, hotspot NRAS, and loss-of-function mutations are found approximately 50%, 25%, 15% cutaneous melanomas, respectively. Compared mutant BRAF role remains understudied. has a RAS GTPase-activating (GAP)...