Gianmarco Piccinno
A5008006450- Gut microbiota and health
- Cancer Immunotherapy and Biomarkers
- Clostridium difficile and Clostridium perfringens research
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Immunotherapy and Immune Responses
- Oral microbiology and periodontitis research
- Probiotics and Fermented Foods
- Nutritional Studies and Diet
- Genetic factors in colorectal cancer
- Ferroptosis and cancer prognosis
- Inflammatory Biomarkers in Disease Prognosis
- Cancer Cells and Metastasis
- Microbial Community Ecology and Physiology
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Gastric Cancer Management and Outcomes
- Cancer, Stress, Anesthesia, and Immune Response
- Effects of Radiation Exposure
- Epigenetics and DNA Methylation
- Colorectal Cancer Screening and Detection
- Adipokines, Inflammation, and Metabolic Diseases
- Dietary Effects on Health
- Agriculture Sustainability and Environmental Impact
- Colorectal Cancer Treatments and Studies
University of Trento
2021-2025
Abstract Metagenomic assembly enables new organism discovery from microbial communities, but it can only capture few abundant organisms most metagenomes. Here we present MetaPhlAn 4, which integrates information metagenome assemblies and isolate genomes for more comprehensive metagenomic taxonomic profiling. From a curated collection of 1.01 M prokaryotic reference metagenome-assembled genomes, define unique marker genes 26,970 species-level genome bins, 4,992 them taxonomically unidentified...
The gut microbiota influences the clinical responses of cancer patients to immunecheckpoint inhibitors (ICIs). However, there is no consensus definition detrimental dysbiosis. Based on metagenomics (MG) sequencing 245 non-small cell lung (NSCLC) patient feces, we constructed species-level co-abundance networks that were clustered into species-interacting groups (SIGs) correlating with overall survival. Thirty-seven and forty-five MG species (MGSs) associated resistance (SIG1) response (SIG2)...
Abstract As plant-based diets gain traction, interest in their impacts on the gut microbiome is growing. However, little known about diet-pattern-specific metagenomic profiles across populations. Here we considered 21,561 individuals spanning 5 independent, multinational, human cohorts to map how differences diet pattern (omnivore, vegetarian and vegan) are reflected microbiomes. Microbial distinguished these common patterns well (mean AUC = 0.85). Red meat was a strong driver of omnivore...
Abstract Metagenomic assembly enables novel organism discovery from microbial communities, but most metagenomes it can only capture few abundant organisms. Here, we present a method - MetaPhlAn 4 to integrate information both metagenome assemblies and isolate genomes for improved more comprehensive metagenomic taxonomic profiling. From curated collection of 1.01M prokaryotic reference metagenome-assembled genomes, defined unique marker genes 26,970 species-level genome bins, 4,992 them...
Tumor immunosurveillance plays a major role in melanoma, prompting the development of immunotherapy strategies. The gut microbiota composition, influencing peripheral and tumoral immune tonus, earned its credentials among predictors survival melanoma. MIND-DC phase III trial (NCT02993315) randomized (2:1 ratio) 148 patients with stage IIIB/C melanoma to adjuvant treatment autologous natural dendritic cell (nDC) or placebo (PL). Overall, 144 collected serum stool samples before after 2...
Highlights•Off-target ILDR synergizes with anti-PD-L1 to eradicate non-irradiated tumors•The abscopal effects of depend on dosimetry (≥1 and ≤3 Gy) volume•Gut composition governs the combined anti-PD-L1•ILDR Christensenellaceae influence emigration intestinal mregDC TdLNSummaryThe mechanisms governing local radiotherapy in cancer patients remain an open conundrum. Here, we show that off-target low-dose irradiation (ILDR) increases clinical benefits immune checkpoint inhibitors or...
Deviations in the diversity and composition of gut microbiota are called "gut dysbiosis". They have been linked to various chronic diseases including cancers resistance immunotherapy. Stool shotgun based-metagenomics informs on ecological prevalence homeostatic bacteria such as Akkermansia muciniphila (Akk), while determination serum addressin MAdCAM-1 instructs endothelial barrier dysfunction. Here we examined patient survival during chemo-immuno-therapy 955 cancer patients across four...
Abstract Background Chronic inflammation, which can be modulated by diet, is linked to high white blood cell counts and correlates with higher cardiometabolic risk of more severe infections, as in the case COVID-19. Methods Here, we assessed association between profile (lymphocytes, basophils, eosinophils, neutrophils, monocytes total cells) markers chronic habitual diet gut microbiome composition (determined sequencing 16S RNA) 986 healthy individuals from PREDICT-1 nutritional intervention...
Abstract Colorectal cancer (CRC) is the third most common and lethal type worldwide. Although CRC incidence in older population has decreased many countries, early-onset cases (< 50 years age, EOCRC) have been rapidly increasing globally. The fecal microbiome reproducibly associated with CRC. However, differences according to age of onset, stage, anatomic location, genomic instability remain only minimally explored. Here, we integrated data generated from ONCOBIOME consortium, Cancer...
Background: Trials have shown that FMT plus immune checkpoint inhibitors (ICI) could improve ⍺PD-1 response in melanoma. The microbiome and shift, along with the clinical impact of non-small cell lung cancer (NSCLC) combination ⍺PD-1+⍺CTLA-4 (dual ICI) melanoma is unknown. Methods: 20 patients (pts) advanced NSCLC treated first-line (1L) 1L dual ICI were recruited. from 1 healthy donor (HD) was administered orally to each pt post bowel prep. week later initiated. A total 11 different HD...
Cancerous tissue is a largely unexplored microbial niche that provides unique environment for the colonization and growth of specific bacterial communities, with it, opportunity to identify novel species. Here, we report distinct features Fusobacterium species, F. sphaericum sp. nov. (Fs), isolated from primary colon adenocarcinoma tissue. We acquire complete closed genome associated methylome this organism phylogenetically confirm its classification into genus, perfoetens as closest...
3534 Background: Gut microbiome has emerged as a biomarker of clinical benefit to immune-checkpoint inhibitors (ICI) but no data are available in metastatic colorectal cancer (mCRC). The AtezoTRIBE study demonstrated that the addition atezolizumab (atezo) FOLFOXIRI plus bevacizumab (bev) prolongs progression-free survival (PFS), this is limited for patients with proficient mismatch repair (pMMR) tumors. Here, we aimed at investigating potential predictive role identifying mCRC able achieve...
719 Background: The LIPI score has been reported as an independent prognostic factor in RCC patients treated with immune checkpoint inhibitors (ICI) or tyrosine kinase (TKI). Here, we aimed to correlate and GM composition RCC. Methods: We prospectively collected fecal samples of all comers who started a 1 st beyond line therapy (standard clinical trial) the NCT0457446 at Gustave Roussy. Neutrophil lymphocyte ratio (dNLR) lactate dehydrogenase (LDH) were obtained from routine blood tests....
Abstract Background: Melanoma is a malignancy where tumor immunosurveillance plays major prognostic role, prompting the development of immunotherapy strategies, dominated earlier on by dendritic cell (DC)-based and recently, immune checkpoint inhibitors (ICI). The taxonomic composition gut microbiome earned its credentials among predictors survival in melanoma, influencing peripheral tumoral tonus. Methods: In MIND-DC phase III clinical trial (NCT02993315), 148 stage IIIB/IIIC cutaneous...
Cancerous tissue is a largely unexplored microbial niche that provides unique environment for the colonization and growth of specific bacterial communities, with it, opportunity to identify novel species. Here, we report distinct features
The vast majority of bacteria that can be grown in a laboratory remain genetically intractable, beyond the power genetics for elucidating function or engineering human use. Inherent diversity genetic defenses, primarily Restriction-Modification (RM) systems, across bacterial species and individual strains remains fundamental barrier to human-made DNA constructs during bacteria. Previously, we described an approach evade RM systems through creation SyngenicDNA; de-novo synthesized sequences...