A5045746270- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Alcoholism and Thiamine Deficiency
- Colorectal Cancer Treatments and Studies
- Air Quality and Health Impacts
- Retinal Diseases and Treatments
- Circular RNAs in diseases
- Helicobacter pylori-related gastroenterology studies
- Gut microbiota and health
- Genetic factors in colorectal cancer
- Mycobacterium research and diagnosis
- RNA Research and Splicing
- Health, Environment, Cognitive Aging
- Neurological and metabolic disorders
- Metabolism and Genetic Disorders
- Liver Disease Diagnosis and Treatment
- Neonatal Health and Biochemistry
- Metabolomics and Mass Spectrometry Studies
- Nutritional Studies and Diet
- Colorectal Cancer Screening and Detection
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Vitamin K Research Studies
- Extracellular vesicles in disease
- Cancer, Lipids, and Metabolism
Italian institute for Genomic Medicine
2016-2025
Candiolo Cancer Institute
2020-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2023
University of Turin
2008-2012
Dysbiotic configurations of the human gut microbiota have been linked to colorectal cancer (CRC). Human small noncoding RNAs are also implicated in CRC, and recent findings suggest that their release lumen contributes shape microbiota. Bacterial (bsRNAs) may play a role carcinogenesis, but has less extensively explored. Here, we performed RNA shotgun sequencing on 80 stool specimens from patients with CRC or adenomas healthy subjects collected cross-sectional study evaluate combined use as...
MicroRNA (miRNA) profiles have been evaluated in several biospecimens relation to common diseases for which diet may a considerable impact. We aimed at characterising how specific diets are associated with the miRNome stool of vegans, vegetarians and omnivores this is reflected gut microbial composition, as still poorly explored.We performed small RNA shotgun metagenomic sequencing faecal samples dietary recording from 120 healthy volunteers, equally distributed different matched sex age.We...
Background & AimsFecal tests currently used for colorectal cancer (CRC) screening show limited accuracy in detecting early tumors or precancerous lesions. In this respect, we comprehensively evaluated stool microRNA (miRNA) profiles as biomarkers noninvasive CRC diagnosis.MethodsA total of 1273 small RNA sequencing experiments were performed multiple biospecimens. a cross-sectional study, miRNA investigated fecal samples from an Italian and Czech cohort (155 CRCs, 87 adenomas, 96 other...
Abstract Background Molecular techniques can complement conventional spermiogram analyses to provide new information on the fertilizing potential of spermatozoa and identify early alterations due environmental pollution. Methods Here, we present a multilevel molecular profiling by small RNA sequencing sperm nuclear basic protein analysis male germ cells from 33 healthy young subjects residing in low high-polluted areas. Results Although motility concentration were comparable between samples...
Diet has a strong influence on many physiological processes, which in turn have important implications variety of pathological conditions. In this respect, microRNAs (miRNAs), class small non-coding RNAs playing relevant epigenetic role controlling gene expression, may represent mediators between the dietary intake and healthy status. Despite great advances field nutri-epigenomics, it remains unclear how miRNA expression is modulated by diet and, specifically, specific nutrients. We...
Current treatment for celiac disease (CD) is adhering to a gluten-free diet (GFD), although its long-term molecular effects are still undescribed. New features detectable in stool may improve and facilitate noninvasive clinical management of CD. For this purpose, fecal small non-coding RNAs (sncRNAs) gut microbiome profiles were concomitantly explored CD subjects relation strict (or not) GFD adherence over time. In observational study, we performed RNA shotgun metagenomic sequencing from 63...
The role of non-coding RNAs in different biological processes and diseases is continuously expanding. Next-generation sequencing together with the parallel improvement bioinformatics analyses allows accurate detection quantification an increasing number RNA species. With aim exploring new potential biomarkers for disease classification, a clear overview expression levels common/unique small species among biospecimens necessary. However, except miRNAs plasma, there are no substantial...
MicroRNAs (miRNAs), a class of small non-coding RNAs, are fundamental for the post-transcriptional regulation gene expression. Altered expression miRNAs has been detected in cancers, not only primary tissue but also easily obtainable specimens like plasma and stools. miRNA is known to be modulated by diet (micro macronutrients, phytochemicals) possibly other lifestyle factors; however, such influence yet exhaustively explored humans. In present study, we analysed levels panel seven human...
Dietary interventions have been proposed as therapeutic approaches for several diseases, including cancer. A low-inflammatory Mediterranean dietary intervention, conducted a pilot study in subjects with Familial Adenomatous Polyposis (FAP), reduced markers of local and systemic inflammation. We aim to determine whether this diet may modulate faecal microRNA (miRNA) gene expression the gut.
Stool samples for fecal immunochemical tests (FIT) are collected in large numbers worldwide as part of colorectal cancer screening programs. Employing FIT from 1034 CRCbiome participants, recruited a Norwegian study, we identify, annotate and characterize more than 18000 DNA viruses, using shotgun metagenome sequencing. Only six percent them assigned to known taxonomic family, with Microviridae being the most prevalent viral family. Linking individual profiles comprehensive lifestyle...
Early detection of colorectal cancer (CRC) significantly improves its management and patients' survival. Circular RNAs (circRNAs) are peculiar covalently closed transcripts involved in gene expression modulation whose dysregulation has been extensively reported CRC cells. However, little is known about their alterations the early phases carcinogenesis. In this study, we performed an integrative analysis circRNA profiles RNA-sequencing (RNA-Seq) data 96 cancers, 27 adenomas, matched adjacent...
The development of colorectal cancer (CRC) is largely influenced by hereditary factors, with up to one-third cases linked genetic predisposition. In parallel, environmental factors such as diet and intestinal microbiota play a significant role. Lynch syndrome (LS), the most common form CRC, due mutations in DNA mismatch repair genes. Diet interventions calorie restriction (CR) can modify course disease, altering nutrient supply promoting beneficial microbial populations. Fasting-mimicking...
Abstract Colorectal cancer (CRC) is one of the leading causes death worldwide, but large screening programs can effectively reduce both incidence and mortality. Fecal immunochemical test (FIT) widely used in CRC cost-effective. However, FIT shows a low sensitivity for advanced adenomas (AA) also prone to false positive results, resulting unnecessary total colonoscopies (TC). Leftover from samples after hemoglobin measurement may offer unique opportunity study alternative biomarkers, which...
Abstract Colorectal cancer (CRC) is the third most common and lethal type worldwide. Although CRC incidence in older population has decreased many countries, early-onset cases (< 50 years age, EOCRC) have been rapidly increasing globally. The fecal microbiome reproducibly associated with CRC. However, differences according to age of onset, stage, anatomic location, genomic instability remain only minimally explored. Here, we integrated data generated from ONCOBIOME consortium, Cancer...
Abstract Background Early and selective loss of pericytes thickening the basement membrane are hallmarks diabetic retinopathy. We reported reduced adhesion, but no changes in apoptosis, bovine retinal cultured on extracellular matrix (ECM) produced by endothelial cells high glucose (HG). Since human may behave differently conditions mimicking milieu, we verified behaviour HG‐conditioned ECM. Methods Pericytes were physiological/HG ECM umbilical vein physiological/HG, alone or presence...