Abstract The use of optical coherence tomography (OCT) to study ocular diseases associated with choroidal physiology is sharply limited by the lack available automated segmentation tools. Current research largely relies on hand-traced, single B-Scan segmentations because commercially programs require high quality images, and existing implementations are closed, scarce not freely available. We developed implemented a robust algorithm for segmenting quantifying layer from 3-dimensional OCT...

Vision loss caused by retinal diseases affects hundreds of millions individuals worldwide. The retina is a delicate central nervous system tissue stratified into layers cells with distinct roles. Currently, there void in treatments that selectively target diseased cells, and current therapeutic paradigms present complications associated off-target effects. Herein, as proof concept, we introduce an vivo method using femtosecond laser to locally optoporate ganglion (RGCs) targeted...

We have developed a novel optical approach to determine pulsatile ocular volume changes using automated segmentation of the choroid, which, together with Dynamic Contour Tonometry (DCT) measurements intraocular pressure (IOP), allows estimation rigidity (OR) coefficient. Spectral Domain Optical Coherence Tomography (OCT) videos were acquired Enhanced Depth Imaging (EDI) at 7Hz during ~50 seconds fundus. A algorithm based on graph search an edge-probability weighting scheme was measure...

Abstract The ability to conduct image-based, non-invasive cell tagging, independent of genetic engineering, is key biology applications. Here we introduce labelling via photobleaching (CLaP), a method that enables instant, specific tagging individual cells based on wide array criteria such as shape, behaviour or positional information. CLaP uses laser illumination crosslink biotin onto the plasma membrane, coupled with streptavidin conjugates label for genomic, cell-tracking, flow cytometry...

HiLo microscopy is a recently developed technique that provides both optical sectioning and fast imaging with simple implementation at very low cost. The methodology combines widefield speckled illumination images to obtain one optically sectioned image. Hence, the characteristics of such speckle ultimately determine quality overall performance method. In this work, we study how contrast influence local variations fluorescence intensity brightness profiles thick samples. We present article...

Neutrophil recruitment guided by chemotactic cues is a central event in host defense against infection and tissue injury. While the mechanisms underlying neutrophil chemotaxis have been extensively studied, these are just recently being addressed using high-content approaches or surface-bound gradients (haptotaxis) vitro. Here, we report haptotaxis assay, based on classic under-agarose which combines an optical patterning technique to generate formyl peptide as well automated imaging...

Purpose: Ocular rigidity (OR) is an important biomechanical property, thought to be relevant in the pathophysiology of open-angle glaucoma (OAG). This study aims evaluate relationship between OR and neuroretinal damage caused by glaucoma. Methods: One hundred eight subjects (22 with healthy eyes, 23 suspect discs, 63 OAG) were included this study. was measured using a noninvasive optical coherence tomography (OCT)-based method developed our group. We also central corneal thickness (CCT),...

Preclinical studies of vascular retinal diseases rely on the assessment developmental dystrophies in oxygen induced retinopathy rodent model. The quantification vessel tufts and avascular regions is typically computed manually from flat mounted retinas imaged using fluorescent probes that highlight network. Such manual measurements are time-consuming hampered by user variability bias, thus a rapid objective method needed. Here, we introduce machine learning approach to segment characterize...

The performance of the single particle tracking (SPT) nearest-neighbor algorithm is determined by parameters that need to be set according characteristics time series under study. Inhomogeneous systems, where these fluctuate spatially, are poorly tracked when globally.We present a novel SPT approach adapts well-known local density particles overcome problems inhomogeneity.We demonstrate improvement provided proposed method using numerical simulations and experimental data compare its with...

Retinopathy of prematurity (ROP) is the leading cause blindness in neonates. Inflammation, particular interleukin-1β (IL-1β), increased early stages disorder, and contributes to inner outer retinal vasoobliteration oxygen-induced retinopathy (OIR) model ROP. A small peptide antagonist IL-1 receptor, composed amino acid sequence, rytvela, has been shown exert beneficial anti-inflammatory effects without compromising immunovigilance-related NF-κB reproductive tissues. We conducted a...

Spectral-domain optical coherence tomography (SD-OCT) is widely used in clinical ophthalmology and recently gained popularity laboratory research involving small rodents. Its noninvasive nature allows repeated measurements, thereby decreasing the number of animals required. However, when at a conventional dosage, xylazine (an α2-adrenoceptor) can cause irreversible corneal calcification, especially among young In present study, we test whether calcification associated with mediated by...

We present a photon counting experiment designed for an undergraduate physics laboratory. The statistics of the number photons pseudo-thermal light source is studied in two limiting cases: much longer and shorter than coherence time, giving Poisson Bose-Einstein distributions, respectively. can be done reasonable time using digital oscilloscope without need boards. use has advantage allowing storage data further processing. stochastic nature detection phenomena adds additional value because...

There is an increasing interest in measuring the peripheral optical quality of eye.Optical aberrations have been studied extensively center visual field due to development Hartmann-Shack wavefront sensor.However, experimental data view are still scarce, partly fact that this evaluation presents various challenges.Here, we propose a novel device based on laser ray-tracing (LRT) aberrometer, which well suited for off-axis aberrations.The proposed instrument able measure wide (T40-) 2D and...

To evaluate the non-invasive measurement of ocular rigidity (OR), an important biomechanical property eye, as a predictor intraocular pressure (IOP) elevation after anti-vascular endothelial growth factor (anti-VEGF) intravitreal injection (IVI).Subjects requiring IVI anti-VEGF for pre-existing retinal condition were enrolled in this prospective cross-sectional study. OR was assessed 18 eyes participants by pulsatile choroidal volume change using video-rate optical coherence tomography, and...

An experimental and theoretical analysis of the angular dependence diffracted light beams emerging from three-dimensional colloidal photonic crystals is herein presented. Diffracted are identified according to their associated reciprocal-lattice vectors, intensities obtained as a function zenithal azimuthal incidence angles. Significant changes in beam observed for large angles angle varied. This phenomenon related excitation new resonant modes inside crystal which cannot be under normal conditions.