A5083968233- Glioma Diagnosis and Treatment
- Enzyme function and inhibition
- Neurological disorders and treatments
- Cancer, Hypoxia, and Metabolism
- Renin-Angiotensin System Studies
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- EEG and Brain-Computer Interfaces
- Chromatin Remodeling and Cancer
- Neuroscience and Neuropharmacology Research
- Epilepsy research and treatment
- Cholinesterase and Neurodegenerative Diseases
- Traumatic Brain Injury and Neurovascular Disturbances
- Vagus Nerve Stimulation Research
- Microtubule and mitosis dynamics
- Radiomics and Machine Learning in Medical Imaging
- Meningioma and schwannoma management
- Neuroinflammation and Neurodegeneration Mechanisms
- Synthesis and Catalytic Reactions
- Hormonal Regulation and Hypertension
- Parkinson's Disease Mechanisms and Treatments
- Cancer-related molecular mechanisms research
- Redox biology and oxidative stress
- interferon and immune responses
- Single-cell and spatial transcriptomics
Tampere University
2015-2025
Tampere University Hospital
2015-2025
Fimlab (Finland)
2014-2024
Hospital for Sick Children
2020-2021
SickKids Foundation
2020-2021
The University of Texas MD Anderson Cancer Center
2017
Center for Neurosciences
2016
Saint Louis University
2008
Slovak Academy of Sciences
2008
Institute of Virology of the Slovak Academy of Sciences
2008
Vagus nerve stimulation (VNS) is used for treating refractory epilepsy and major depression. While the impact of this treatment on seizures has been established, its human cognition remains equivocal. The goal study to elucidate immediate effects vagus attention, cognition, emotional reactivity in patients with epilepsy. Twenty (12 male 8 female; 45 ± 13 years old) treated VNS due participated study. Subjects performed a computer-based test executive functions embedded distractors while...
BackgroundDeep brain stimulation of the anterior nucleus thalamus (ANT) is an emerging therapy for refractory focal epilepsy. However, most optimal target has not been unambiguously described.ObjectiveIn present study, we investigated correlation between site and outcome in order to define deep epilepsy.MethodsThe locations 62 contacts used 30 treatment attempts 15 prospectively followed patients during a 5 year period were assessed. Treatment classified into responding non-responding trials...
Abstract Purpose: Carbonic anhydrase IX (CA IX) is a hypoxia-inducible enzyme, which associated with neoplastic growth. Ectopic CA expression has been observed in several tumors, whose normal counterparts do not express this enzyme. Normal human brain tissue shows only slight or no of IX. Experimental Design: We describe diffusely infiltrating astrocytomas. The association evaluated clinicopathologic and molecular factors including cell proliferation apoptosis as well the p53 epidermal...
Deep brain stimulation (DBS) is a minimally invasive and reversible method to treat an increasing number of neurological psychiatric disorders, including epilepsy. Targeting poorly defined deep structures based in large degree on stereotactic atlas information, which may be major source inconsistent treatment effects. In the present study, we aimed study whether recently approved target for epilepsy (anterior nucleus thalamus, ANT) visualized clinically established 3 T MRI ANT delineated...
Background: Deep brain stimulation (DBS) of anterior thalamic nuclei (ANT) is a novel promising therapeutic method for treating refractory epilepsy. Despite reports subjective memory impairments and mood disturbances in patients with ANT–DBS, little known its effects on cognitive affective processes. Hypothesis: The thalamus has connections to prefrontal limbic networks important control emotional reactivity. More specifically, cingulate cortex (ACC), linked ANT, been assigned roles related...
Rapid technological advancements have transformed the healthcare sector from traditional diagnosis and treatment to personalized health management. Biofluids such as teardrops, sweat, interstitial fluids, exhaled breath condensate offer a rich source of metabolites that can be linked physiological status an individual. More importantly, these biofluids contain biomarkers similar those in blood. Therefore, developing sensors for noninvasive determination biofluid‐based overcome traditionally...
Carbonic anhydrase XII (CA XII) is a transmembrane enzyme that associated with neoplastic growth. CA has been proposed to be involved in acidification of the extracellular milieu, creating an appropriate microenvironment for rapid tumor Because RNA sequence databases have indicated two isoforms might exist human tissues, and because alternatively spliced protein forms linked aggressive behavior cancer cells, we designed study evaluate presence diffuse astrocytomas, type known its often...
Abstract Background Defective iron homeostasis may be involved in the development of some diseases within central nervous system. Although expression genes normal balance has been intensively studied other tissues, little is known about their brain. We investigated mRNA levels hepcidin ( HAMP ), HFE, neogenin NEO1 transferrin receptor 1 TFRC 2 TFR2 and hemojuvelin HFE2 ) human brain, brain tumors, astrocytoma cell lines. The specimens included 5 tissue samples, 4 meningiomas, one...
Medulloblastomas (MBs) and supratentorial primitive neuroectodermal tumours (PNETs) are the most common highly aggressive paediatric brain tumours. In spite of extensive research on these tumours, there only few known biomarkers or therapeutic target proteins, prognosis patients with remains poor. Our aim was to investigate whether carbonic anhydrases (CAs), enzymes commonly overexpressed in various including glioblastomas oligodendrogliomas, present MBs PNETs, their expression can be...
Abstract The tumor immune microenvironment (TiME) of human central nervous system (CNS) tumors remains to be comprehensively deciphered. Here, we employed flow cytometry and RNA sequencing analysis for a deep data-driven dissection diverse TiME uncover noncanonical cell types in CNS by using seven from five patients. Myeloid subsets comprised classical microglia, monocyte-derived macrophages, neutrophils, two myeloid subsets: CD3 + myeloids CD19 myeloids. T lymphocyte included...
A substantial proportion of patients undergoing surgery for chronic subdural hematoma (CSDH) use anticoagulation medication due to atrial fibrillation (AF). We assessed the risk postoperative thromboembolic and hemorrhagic complications in CSDH with a history AF their association outcome. This posthoc analysis nationwide multicenter randomized controlled trial conducted during 2020-2022 included preoperative AF. incidence associations functional outcomes mortality. Of 589 patients, 128...
Carbonic anhydrase isozyme II (CA II) is a cytosolic enzyme that highly expressed in most organs, including the brain, where it mainly located oligodendrocytes. Recent studies have shown its expression induced endothelium of neovessels melanoma and esophageal, renal, lung cancer. Immunological further indicate CA represents major target antigen stimulating an autoantibody response patients. These results prompted us to investigate endothelial two types brain cancer: oligodendrogliomas...
Carbonic anhydrase VII (CA VII) is a cytosolic enzyme expressed in several organs, including the human brain, but it has not been investigated earlier any tumors. We designed present study to evaluate CA expression cohort of diffuse astrocytomas, mixed oligoastrocytomas and oligodendrogliomas. immunostaining was correlated clinico-pathologic findings, survival data, expres-sion other molecular factors, Ki-67, p53 protein epidermal growth factor receptor. VII-positive staining observed 94%...
Super-refractory status epilepticus is a condition characterized by recurrence of despite use deep general anesthesia, and it has high morbidity mortality rates. We report case 17-year-old boy with prolonged super-refractory that eventually resolved after commencing brain stimulation the centromedian nucleus thalamus. Later attempt to reduce parameters resulted in immediate relapse epilepticus, suggesting pivotal role treatment response. Deep may be option when other options have failed. ANN...
Abstract Objectives Neurostimulation has offered new treatment options in refractory epilepsy, first with vagus nerve stimulation ( VNS ) and more recently deep brain DBS ). There is a lack of previous detailed data assessing the relationship between ANT ‐ . The aim this study was to investigate potential correlation therapeutic responses Materials Methods A total 11 patients therapy underwent implantation. Monthly seizure counts starting from baseline before extending long‐term were...
Chromogenic in situ hybridization (CISH) was used to detect amplification of the epidermal growth factor receptor (EGFR) gene tissue microarrays tumours derived from 287 patients with grade II-IV diffuse astrocytomas. Amplification found 32% a highly significant association histological (4% II, 21% III and 39% IV; P < 0.001). EGFR more common primary than secondary glioblastomas (41%vs. 16%, = 0.033). Overexpression mRNA protein (wild-type vIII variant) correlate (P 0.028, 0.035 0.014...
Inhibitors of fibroblast growth factor receptors (FGFRs) have recently arisen as a promising treatment option for patients with FGFR alterations. Gene fusions involving FGFR3 and transforming acidic coiled-coil protein 3 (TACC3) been detected in diffuse gliomas other malignancies, fusion-positive cases responded well to inhibition. As high expression has tumors, we sought determine the clinical significance level its potential indicating fusions.We performed immunohistochemistry on tissue...
As the progression of low-grade diffuse astrocytomas into grade 4 tumors significantly impacts patient prognosis, a better understanding this process is paramount importance for improved care. In project, we analyzed matched IDH-mutant before and after to from six patients (discovery cohort) with genome-wide sequencing, 21 additional targeted 33 Glioma Longitudinal AnalySiS cohort validation. The Cancer Genome Atlas data 595 gliomas provided supportive information. All in our discovery...
Atypical teratoid/rhabdoid tumors (AT/RTs) are pediatric brain known for their aggressiveness and aberrant but still unresolved epigenetic regulation. To better understand malignancy, we investigated how AT/RT-specific DNA hypermethylation was associated with gene expression altered transcription factor binding it is linked to upstream Medulloblastomas, choroid plexus tumors, pluripotent stem cells, fetal were used as references. A part of the genomic regions, which hypermethylated in AT/RTs...