A5071105199- S100 Proteins and Annexins
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Cancer, Lipids, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Cholesterol and Lipid Metabolism
- Ion channel regulation and function
- Caveolin-1 and cellular processes
- Lipoproteins and Cardiovascular Health
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Connexins and lens biology
- Drug Transport and Resistance Mechanisms
- Peroxisome Proliferator-Activated Receptors
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and biosynthesis
- Cardiovascular Issues in Pregnancy
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid metabolism and disorders
- Animal Genetics and Reproduction
The University of Sydney
2016-2025
UNSW Sydney
2004-2022
Columbia College
2013
Royal College of Physicians
2013
Columbia University
2013
University of Saskatchewan
2003-2007
St Vincent's Hospital Sydney
2004-2007
St Vincent's Hospital
2005-2006
University Medical Center Hamburg-Eppendorf
2000-2005
Universität Hamburg
1998-2005
Obesity is associated with increased recurrence and reduced survival of breast cancer. Adipocytes constitute a significant component tissue, yet their role in provisioning metabolic substrates to support cancer progression poorly understood. Here, we show that co-culture cells adipocytes revealed cell-stimulated depletion adipocyte triacylglycerol. Adipocyte-derived free fatty acids were transferred cells, driving acid metabolism via CPT1A electron transport chain complex protein levels,...
Annexins are cytosolic proteins with conserved three-dimensional structures that bind acidic phospholipids in cellular membranes at elevated Ca
Alcoholic and nonalcoholic liver steatosis steatohepatitis are characterized by the massive accumulation of lipid droplets (LDs) in cytosol hepatocytes. Although LDs ubiquitous dynamic organelles found cells a wide range organisms, little is known about mechanisms sites LD biogenesis. To examine participation these pathophysiological disorders steatotic livers, we used combination mass spectrometry (matrix‐assisted laser desorption ionization‐time flight LC‐MS electrospray) Western blot...
Inhibition of cholesterol export from late endosomes causes cellular imbalance, including depletion in the trans-Golgi network (TGN). Here, using Chinese hamster ovary (CHO) Niemann-Pick type C1 (NPC1) mutant cell lines and human NPC1 fibroblasts, we show that altered levels at TGN/endosome boundaries trigger Syntaxin 6 (Stx6) accumulation into VAMP3, transferrin, Rab11-positive recycling (REs). This increases Stx6/VAMP3 interaction interferes with αVβ3 α5β1 integrins migration, possibly a...
After internalization of triglyceride-rich lipoproteins (TRL) in hepatoma cells, TRL particles are immediately disintegrated the early endosomal compartment. This involves targeting lipids and apoprotein B along degradative pathway recycling TRL-derived apoE through endosomes. Re-secretion is accompanied by concomitant association cellular cholesterol with high-density (HDL). Since epidemiological data showed that apoE3 apoE4 have differential effects on HDL metabolism, we investigated...
The T lymphocyte plasma membrane condenses at the site of activation but functional significance this receptor-mediated reorganization is not yet known. Here we demonstrate that condensation cell sites can be inhibited by incorporation oxysterol 7-ketocholesterol (7KC), which known to prevent formation raft-like liquid-ordered domains in model membranes. We enriched cells with 7KC, or cholesterol as control, assess importance for activation. Upon 7KC treatment, antigen receptor (TCR)...
Annexin A6 (AnxA6) belongs to a family of Ca 2+ ‐dependent membrane‐binding proteins and is involved in the regulation endocytic exocytic pathways. We previously demonstrated that AnxA6 regulates receptor‐mediated endocytosis lysosomal targeting low‐density lipoproteins translocates cholesterol‐enriched late endosomes (LE). As cholesterol modulates membrane binding cellular location AnxA6, but also affects intracellular distribution caveolin, we investigated localization trafficking caveolin...
Intracellular chloride channel protein 1 (CLIC1) is a 241 amino acid of the glutathione S transferase fold family with redox- and pH-dependent membrane association ion activity. Whilst CLIC proteins are evolutionarily conserved in Metazoa, indicating an important role, little known about their biology. CLIC1 was first cloned on basis increased expression activated macrophages. We therefore examined its subcellular localisation murine peritoneal macrophages by immunofluorescence confocal...
ABSTRACT To transfer the viral genome into host cell cytoplasm, internalized influenza A virus (IAV) particles depend on fusion of IAV envelope with endosomal membranes. The antiviral interferon (IFN) response includes upregulation interferon-induced transmembrane protein 3 (IFITM3), which inhibits release content cytosol. Although IFITM3 induction occurs concomitantly late endosomal/lysosomal (LE/L) cholesterol accumulation, functional significance this process is not well understood. Here...
Abstract Cholesterol accumulation in late endosomes is a prevailing phenotype of Niemann-Pick type C1 (NPC1) mutant cells. Likewise, annexin A6 (AnxA6) overexpression induces reminiscent NPC1 Here, we demonstrate that this cellular cholesterol imbalance due to AnxA6 promoting Rab7 inactivation via TBC1D15, Rab7-GAP. In cells, depletion and eventual activation was associated with peripheral distribution increased mobility endosomes. This accompanied by an enhanced lipid droplets...
Prostate cancer growth is driven by androgen receptor signaling, and advanced disease initially treatable depleting circulating androgens. However, prostate cells inevitably adapt, resulting in relapse with incurable castrate-resistant cancer. Androgen deprivation therapy has many side effects, including hypercholesterolemia, more aggressive cancers typically feature cellular accumulation of cholesterol stored the form cholesteryl esters. As a key substrate for de novo steroidogenesis cells,...
Annexins are calcium-binding proteins with a wide distribution in most polarized and nonpolarized cells that participate variety of membrane-membrane interactions. At the cell surface, annexin VI is thought to remodel spectrin cytoskeleton facilitate budding coated pits. However, also found late endocytic compartments number types, indicating an additional important role at later stages pathway. Therefore overexpression Chinese hamster ovary was used investigate its possible endocytosis...
Annexins are Ca(2+)- and phospholipid-binding proteins that widely expressed in mammalian tissues bind to different cellular membranes. In recent years its role membrane traffic has emerged as one of predominant functions, but the regulation intracellular distribution still remains unclear. We demonstrated annexin 6 translocates late endocytic compartment low density lipoprotein-loaded CHO cells. This prompted us investigate whether cholesterol, major constituents lipoprotein, could...