A5065224125- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Computational Drug Discovery Methods
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Phytochemicals and Antioxidant Activities
- Essential Oils and Antimicrobial Activity
- Genomics, phytochemicals, and oxidative stress
- RNA Interference and Gene Delivery
- Synthesis and biological activity
- S100 Proteins and Annexins
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- DNA Repair Mechanisms
- Antioxidant Activity and Oxidative Stress
- Glioma Diagnosis and Treatment
- Phytochemistry and Biological Activities
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Wound Healing and Treatments
- Multicomponent Synthesis of Heterocycles
- Mesenchymal stem cell research
- Climate Change and Health Impacts
- Synthesis of heterocyclic compounds
Centre for Cellular and Molecular Biology
2011-2024
Krishna Institute of Medical Sciences
2019-2022
The University of Sydney
2017-2022
Council of Scientific and Industrial Research
2015
Global Hospitals
2013-2015
Jawaharlal Nehru Technological University, Hyderabad
2007-2014
Ragas Dental College & Hospital
2004-2006
The aim of this study was to evaluate the magnitude oxidative stress and levels nitric oxide in patients with oral cavity cancer by analyzing lipid peroxidation products, antioxidants products.This prospective conducted on 15 biopsy proven squamous cell clinical stage III/IV an equal number age sex matched healthy subjects. products were determined colorimetric methods.Lipid like hydroperoxide (LHP) malondialdehyde (MDA) nitrite (NO(2)(-)), nitrate (NO(3)(-)) total (TNO(2)(-)) significantly...
Abstract Cholesterol accumulation in late endosomes is a prevailing phenotype of Niemann-Pick type C1 (NPC1) mutant cells. Likewise, annexin A6 (AnxA6) overexpression induces reminiscent NPC1 Here, we demonstrate that this cellular cholesterol imbalance due to AnxA6 promoting Rab7 inactivation via TBC1D15, Rab7-GAP. In cells, depletion and eventual activation was associated with peripheral distribution increased mobility endosomes. This accompanied by an enhanced lipid droplets...
Acetone and hexane extracts derived from the root, stem, leaf of Raphanus sativus were investigated for their antibacterial activity against foodborne resistant pathogens, such as Bacillus subtilis, Staphylococcus aureus, epidermidis, Enterococcus faecalis, Salmonella typhimurium, Enterobacter aerogenes, cloacae, Escherichia coli. Total individual isothiocyanate (ITC) components relationship with R. also evaluated. Both acetone fractions exhibited selective organisms tested. Antibacterial...
Abstract Background Invasive ductal carcinoma (IDC) is the most common form of breast cancer which accounts for 85% all diagnoses. Non-invasive and early stages have a better prognosis than late-stage invasive that has spread to lymph nodes. The involvement microRNAs (miRNAs) in initiation progression holds great promise development molecular tools diagnosis prognosis. Therefore, developing cost effective, quick robust detection protocol using miRNAs an imminent need could strengthen health...
Raphanus sativus, a common cruciferous vegetable has been attributed to possess number of pharmacological properties. Antioxidant and radical scavenging activity R. sativus root extracted with solvents varying polarity were evaluated using different model systems. Polyphenolic content was estimated be in the range 13.18-63.54 mg g⁻¹ dry weight, considerable amount being obtained polar solvents. High-performance liquid chromatography analysis indicated presence an array polyphenolics....
Psoriasis affects up to 3% of the world's population or more than 125 million people. There is an urgent need for new treatment strategy, as 50% patients are not satisfied with current therapies. We evaluated combined efficiency platelet rich plasma (PRP) and methotrexate (MTX) in management plaque psoriasis. Twenty-one chronic psoriasis were recruited study. Sixteen assigned into combinational group (PRP + MTX) monotherapy (MTX alone) consisted five patients. All therapy received autologous...
In this article, we report the development and preclinical validation of combinatorial therapy for treatment cancers using RNA interference (RNAi). RNAi technology is an attractive approach to silence genes responsible disease onset progression. Currently, critical challenge facing clinical success in difficulty delivery inducers, due low transfection efficiency, difficulties integration into host DNA unstable expression. Using macromolecule polyglycidal methacrylate (PGMA) as a platform...
Abstract Cholesterol is considered indispensable for cell motility, but how physiological cholesterol pools enable cells to move forward remains be clarified. The majority of obtain from the uptake Low-Density lipoproteins (LDL) and here we demonstrate that LDL stimulates A431 squamous epithelial carcinoma Chinese hamster ovary (CHO) migration invasion. also potentiated epidermal growth factor (EGF) -stimulated as well invasion in 3-dimensional environments, using organotypic assays....
Abstract: Fluorescent magnetic iron oxide nanoparticles have been used to label cells for imaging as well therapeutic purposes. The purpose of this study was modify the approach develop a nanoprobe cell selection and with direct translational focus. involves physical coincubation adsorption superparamagnetic nanoparticle-polyethylene glycol (SPION-PEG) complexes monoclonal antibody (mAb) or set antibodies. Flow cytometry, confocal laser scanning microscopy, transmission electron staining,...
Annexin A6 (AnxA6), a member of the calcium (Ca2+ ) and membrane binding annexins, is known to stabilize establish formation multifactorial signaling complexes. At plasma membrane, AnxA6 scaffold for protein kinase Cα (PKCα) GTPase-activating p120GAP promote downregulation epidermal growth factor receptor (EGFR) Ras/mitogen-activated (MAPK) signaling. In human squamous A431 epithelial carcinoma cells, which overexpress EGFR, but lack endogenous AnxA6, restoration expression (A431-A6)...
Directional transdifferentiation of bone marrow precursor cells assumes beta cell like properties in modified tissue microenvironment. The factors that modify the roles to functional enabling precise, defined and efficient vitro differentiation protocols are yet be conclusive. study aims at determination appropriate induction may aid robust, reproducible rat derived mesenchymal stem (MSCs) islet-like enhance their efficiency. High glucose concentration including nicotinamide,...
We report the design and synthesis of 1-aroyl-2-(alkenyl/aryl)idene hydrazines as hybrid molecules derived from mefenamic acid substituted hydrazones. A number compounds based on this new scaffold were prepared in good yields. The key intermediate N-acylhydrazine, acid, was coupled with a variety aldehydes under conventional well microwave irradiation conditions. second approach, that requires short reaction time, can be carried out solvent free condition does not require use an catalyst or...
Abstract Functionalization of quinoline aldehydes, derived from nimesulide framework was carried out using Morita–Baylis–Hillman (MBH) chemistry. A number novel quinoline‐based diverse MBH adducts prepared via the reaction derivatives 2‐chloroquinoline‐3‐carbaldehyde and various activated alkenes in good yields. Many these compounds were found to be potent when tested against human prostate cancer (Pc‐3) cell line vitro . Among all N...
OncomiRs are microRNAs that associated with early onset of specific cancers. To identify involved in pediatric acute lymphoblastic leukemia (ALL) subtypes T-ALL and B-ALL, peripheral blood bone marrow samples were independently subjected to microarray analysis using two different high-fidelity array platforms. The unique common gene signatures from both arrays validated by TaqMan individual assays 100 ALL samples. Survival studies carried out the test set validation 50 randomly selected each...
XPD acts as a functional helicase and aids in unwindingdouble helix around damaged DNA, leading to efficient DNA repair. Mutations of give rise DNA-repair deficiency diseasesand cancer proneness. In this study,cancer-causing missense mutation that could inactivate function hinderits binding with other complexes were analysed usingbioinformatics approach. Rigorous computational methodswere employed understand the molecular pathogenic profile mutation. The mutant model desired was built...
Abstract Background Oxidative stress by reactive oxygen species (ROS) and nitrosative nitrogen (RNS) are proven initiators promoters in carcinogenesis. Elevated ROS/RNS with lowered antioxidants occur patients squamous cell carcinoma of oral cavity. Ours is the first study to evaluate effect curative resection on both oxidative such patients. Methods This was conducted 24 cancer age‐ sex‐matched healthy controls. Lipid peroxidation products, nitric oxide (NO) products ceruloplasmin (CPL)...
We report some hybrid molecules based on N-(quinoline-3-ylmethylene)benzohydrazide template the synthesis of which was carried out using 2-chloroquinoline-3-carbaldehyde and a variety substituted hydrazides in PEG 400. The "green" solvent 400 recovered reused for several times present reaction found to be effective terms product yield. Some compounds synthesized showed significant cytotoxic activity when tested vitro