A5076305049- Lipoproteins and Cardiovascular Health
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular Disease and Adiposity
- Cancer, Lipids, and Metabolism
- Cholesterol and Lipid Metabolism
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cardiovascular Function and Risk Factors
- Adipokines, Inflammation, and Metabolic Diseases
- Protease and Inhibitor Mechanisms
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- Antioxidant Activity and Oxidative Stress
- Cardiomyopathy and Myosin Studies
- Endoplasmic Reticulum Stress and Disease
- Cardiovascular Effects of Exercise
- RNA modifications and cancer
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Mitochondrial Function and Pathology
- Extracellular vesicles in disease
- Nuclear Structure and Function
- Cardiac Fibrosis and Remodeling
- Liver Disease Diagnosis and Treatment
Institut d'Investigacions Biomèdiques de Barcelona
2017-2024
Instituto de Salud Carlos III
2010-2024
Centro de Investigación en Red en Enfermedades Cardiovasculares
2000-2024
Hospital de Sant Pau
2011-2023
Consejo Superior de Investigaciones Científicas
2013-2023
Centro de Investigación Biomédica en Red
2010-2023
Universitat Autònoma de Barcelona
2011-2023
Biomedical Research Institute
2020
Hospital Universitario La Paz
2020
Universidad Autónoma de Madrid
2020
The metabolic effect of intratumor cholesteryl ester (CE) in breast cancer remains poorly understood. objective was to analyze the relationship between CE content and clinicopathological variables human carcinomas.We classified 30 carcinoma samples into three subgroups: 10 luminal-A tumors (ER+/PR+/Her2-), Her-2 (ER-/PR-/Her2+), triple negative (TN) (ER-/PR-/Her2-). We analyzed neutral CE, free cholesterol (FC) triglyceride (TG) by thin layer chromatography after lipid extraction. RNA...
Abstract Contractile dysfunction is underdiagnosed in early stages of diabetic cardiomyopathy. We evaluated the potential circulating long non-coding RNAs (lncRNAs) as biomarkers subclinical cardiac abnormalities type 2 diabetes. Forty-eight men with well-controlled diabetes and 12 healthy age-matched volunteers were enrolled study. Left ventricular (LV) parameters measured by magnetic resonance imaging. A panel lncRNAs was quantified serum RT-qPCR. No differences expression levels observed...
Using in vitro, vivo and patient-based approaches, we investigated the potential of circulating microRNAs (miRNAs) as surrogate biomarkers myocardial steatosis, a hallmark diabetic cardiomyopathy. We analysed cardiomyocyte-enriched miRNA signature serum from patients with well-controlled type 2 diabetes verified absence structural heart disease or inducible ischemia, control volunteers same age range BMI (N = 86), high-fat diet-fed murine model, exosomes lipid-loaded HL-1 cardiomyocytes....
The role of circular RNAs (circRNAs) as biomarkers remains poorly characterized. Here, we investigated the performance circRNA hsa_circ_0001445 a biomarker coronary artery disease (CAD) in real-world clinical practice setting. Plasma was measured study population 200 consecutive patients with suspected stable CAD who had undergone computed tomographic angiography (CTA). Multivariable logistic models were constructed combining conventional risk factors established and hsa_circ_0001445. Model...
The prevalence of nonalcoholic fatty liver disease (NAFLD) has increased drastically due to the global obesity pandemic but at present there are no approved therapies. Here, we aimed revert high-fat diet (HFD)-induced and NAFLD in mice by enhancing acid oxidation (FAO). Moreover, searched for potential new lipid biomarkers monitoring steatosis humans. We used adeno-associated virus (AAV) deliver a permanently active mutant form human carnitine palmitoyltransferase 1A (hCPT1AM), key enzyme...
Abstract —Foam cell formation is a key event in the onset and progression of atherosclerotic lesions. We have previously reported that internalization aggregated low density lipoproteins (agLDLs) by vascular smooth muscle cells (VSMCs) produces cholesteryl ester (CE) accumulation these cells. The aim this study was to analyze whether lipoprotein receptor–related protein (LRP) mediates uptake agLDL VSMCs. First, immunocytochemistry fluorescence microscopic analysis with use anti-LRP...
Abstract Cholesterol accumulation in late endosomes is a prevailing phenotype of Niemann-Pick type C1 (NPC1) mutant cells. Likewise, annexin A6 (AnxA6) overexpression induces reminiscent NPC1 Here, we demonstrate that this cellular cholesterol imbalance due to AnxA6 promoting Rab7 inactivation via TBC1D15, Rab7-GAP. In cells, depletion and eventual activation was associated with peripheral distribution increased mobility endosomes. This accompanied by an enhanced lipid droplets...
The HDL (high-density lipoprotein)-mediated stimulation of cellular cholesterol efflux initiates macrophage-specific reverse transport (m-RCT), which ends in the fecal excretion macrophage-derived unesterified (UC). Early studies established that LDL (low-density lipoprotein) particles could act as efficient intermediate acceptors cellular-derived UC, thereby preventing saturation and facilitating their capacity. However, capacity to a plasma reservoir its potential impact supporting m-RCT...
Tissue factor (TF) is the main initiator of arterial blood coagulation system, and aggregated LDL (agLDL) are found in intima. Our hypothesis that agLDL internalization by vascular smooth muscle cells (VSMCs) may trigger TF-procoagulant activity.Cultured human VSMCs were obtained from coronary arteries explanted hearts during transplant operations. incubated with native (nLDL) or agLDL. TF mRNA was analyzed real-time polymerase chain reaction, cellular released protein antigen Western blot....
Hypoxia is considered a key factor in the progression of atherosclerotic lesions. Low-density lipoprotein receptor-related protein (LRP1) plays pivotal role vasculature. The aim this study was to investigate effect hypoxia on LRP1 expression and function vascular smooth muscle cells (VSMC) hypoxia-inducible factor-α (HIF-1α).Real-time polymerase chain reaction Western blot analysis demonstrated that (1% O(2)) time-dependently induced mRNA (maximum levels at 1 2 hours) 12 24 hours). delayed...
The myocardium accumulates intracellular lipids under ischaemic conditions, and myocardial fat deposition is closely associated with cardiac dysfunction. Our aims were to analyse the effect of hypoxia on low-density lipoprotein receptor-related protein 1 (LRP1) expression in neonatal rat ventricular myocytes (NRVM) cardiac-derived HL-1 cells molecular mechanisms involved this effect, determine role LRP1 very low density (VLDL) uptake by hypoxic cardiomyocytes, study receptor lipid profile an...
Obesity and its associated metabolic comorbidities are a rising global health social issue, with novel therapeutic approaches urgently needed. Adipose tissue plays key role in the regulation of energy balance adipose tissue-derived mesenchymal stem cells (AT-MSCs) have gained great interest cell therapy. Carnitine palmitoyltransferase 1A (CPT1A) is gatekeeper enzyme for mitochondrial fatty acid oxidation. Here, we aimed to generate adipocytes expressing constitutively active CPT1A form...
Versican-like proteoglycans are the main component of intimal extracellular matrix interacting with low density lipoprotein (LDL). The aim this study has been to investigate receptors involved in versican-modified LDL uptake by human vascular smooth muscle cells (VSMCs). We have found that versican-LDL interaction leads following: (1) monomeric particles similar size and electrophoretic mobility native but a higher capacity induce intracellular cholesteryl ester (CE) accumulation (2) fused...
In the extracellular intima, matrix proteoglycans favor LDL retention and aggregation (agLDL). contrast to native (nLDL), agLDL induces high intracellular cholesteryl ester (CE) accumulation in macrophages. It has been suggested that receptor-related protein (LRP1) is involved binding internalization by The aim of this work was analyze whether sterol regulatory element proteins (SREBPs) modulate LRP1 expression LRP1-mediated uptake human monocyte-derived macrophages (HMDM).The treatment HMDM...
Lipoproteins have been described as microRNAs (miRNAs) carriers. Unfortunately, the bibliography on this topic is scarce and shows a high variability between independent investigations. In addition, miRNA profiles of LDL VLDL fractions not completely elucidated. Here, we profiled human circulating lipoprotein-carried miRNome. Lipoprotein (VLDL, HDL) were isolated from serum healthy subjects by ultracentrifugation purified size-exclusion chromatography. A panel 179 miRNAs commonly expressed...