A5072384294- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Advanced Chemical Sensor Technologies
- Lipid metabolism and biosynthesis
- Isotope Analysis in Ecology
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer, Hypoxia, and Metabolism
- Mass Spectrometry Techniques and Applications
- Toxic Organic Pollutants Impact
- DNA Repair Mechanisms
- Ginseng Biological Effects and Applications
- Biochemical and Molecular Research
- Regulation of Appetite and Obesity
- Cancer, Lipids, and Metabolism
- Cardiovascular Function and Risk Factors
- Diet, Metabolism, and Disease
- Heart Rate Variability and Autonomic Control
- Peroxisome Proliferator-Activated Receptors
- Diabetes Management and Research
- Mitochondrial Function and Pathology
- Cardiovascular Disease and Adiposity
- Ovarian function and disorders
- Infant Nutrition and Health
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2012-2024
Instituto de Salud Carlos III
2019-2024
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2012-2024
Universitat Rovira i Virgili
2013-2024
Institut d'Investigació Sanitària Pere Virgili
2011-2022
Centre for Omic Sciences
2015-2016
Several metabolomic software programs provide methods for peak picking, retention time alignment and quantification of metabolite features in LC/MS-based metabolomics. Statistical analysis, however, is needed order to discover those significantly altered between samples. By comparing the MS/MS data a model compound that from feature interest research sample, metabolites can be then unequivocally identified. This paper reports on comprehensive overview workflow statistical analysis rank...
Key nuclear processes in eukaryotes, including DNA replication, repair, and gene regulation, require extensive chromatin remodeling catalyzed by energy-consuming enzymes. It remains unclear how the ATP demands of such are met response to rapid stimuli. We analyzed this question context massive regulation changes induced progestins breast cancer cells found that is generated cell nucleus via hydrolysis poly(ADP-ribose) ADP-ribose. In presence pyrophosphate, ADP-ribose used pyrophosphatase...
Gas chromatography coupled to mass spectrometry (GC/MS) has been a long-standing approach used for identifying small molecules due the highly reproducible ionization process of electron impact (EI). However, use GC-EI MS in untargeted metabolomics produces large and complex data sets characterized by coeluting compounds extensive fragmentation molecular ions caused hard ionization. In order identify extract quantitative information on metabolites across multiple biological samples,...
Abdominal adiposity and obesity influence the association of polycystic ovary syndrome (PCOS) with insulin resistance diabetes. We aimed to characterize intermediate metabolism phenotypes associated PCOS obesity.We applied a nontargeted GC-MS metabolomic approach plasma samples from 36 patients 39 control women without androgen excess, matched for age, body mass index, frequency obesity.Patients were hyperinsulinemic resistant compared controls. The increase in long-chain fatty acids, such...
Because of the wide range chemically and structurally diverse metabolites, efforts to survey complete metabolome rely on implementation multiplatform approaches based nuclear magnetic resonance (NMR) mass spectrometry (MS). Sample preparation disparities between NMR MS, however, may limit analysis same samples by both platforms. Specifically, deuterated solvents used in strategies can complicate LC/MS as a result potential shifts, whereas acidic solutions typically methods enhance ionization...
The beneficial effects of exercise in patients with type 1 diabetes (T1D) are not fully proven, given that it may occasionally induce acute metabolic disturbances. Indeed, the disturbances associated sustained lead to worsening control unless great care is taken adjust carbohydrate intake and insulin dosage. In this work, pre- post-exercise metabolites were analyzed using a 1H-NMR GC-MS untargeted metabolomics approach assayed serum. We studied ten men T1D eleven controls matched for age,...
Studying the flow of chemical moieties through complex set metabolic reactions that happen in cell is essential to understanding alterations homeostasis occur disease. Recently, LC/MS-based untargeted metabolomics and isotopically labeled metabolites have been used facilitate unbiased mapping pathways. However, due complexity resulting experimental data sets few computational tools are available for analysis. Here we introduce geoRge, a novel approach capable analyzing LC/MS from stable...
Upregulation of fatty acid synthase (FASN) is a common event in cancer, although its mechanistic and potential therapeutic roles are not completely understood. In this study, we establish key role FASN during transformation. required for eliciting the anaplerotic shift Krebs cycle observed cancer cells. However, main to consume acetyl-CoA, which unlocks isocitrate dehydrogenase (IDH)-dependent reductive carboxylation, producing power necessary quench reactive oxygen species (ROS) originated...
Abstract The mechanisms that allow breast cancer (BCa) cells to metabolically sustain rapid growth are poorly understood. Here we report BCa dependent on a mechanism supply precursors for intracellular lipid production derived from extracellular sources and the endothelial lipase (LIPG) fulfils this function. LIPG expression allows import of precursors, thereby contributing proliferation. stands out as an essential component metabolic adaptations cells, not normal tissue, must undergo...
The indexed individual, from now on termed M116, was the world's oldest verified living person January 17th 2023 until her passing August 19th 2024, reaching age of 117 years and 168 days (https://www.supercentenarian.com/records.html). She a Caucasian woman born March 4th 1907 in San Francisco, USA, Spanish parents settled Spain since she 8. Although centenarians are becoming more common demographics human populations, so-called supercentenarians (over 110 old) still rarity. In Catalonia,...
Metabolic plasticity is the ability of a biological system to adapt its metabolic phenotype different environmental stressors. We used whole-body and tissue-specific phenotypic, functional, proteomic, metabolomic transcriptomic approach systematically assess in diet-induced obese mice after combined nutritional exercise intervention. Although most obesity overnutrition-related pathological features were successfully reverted, we observed high degree dysfunction visceral white adipose tissue,...
Polycystic ovary syndrome (PCOS) is a variable disorder characterized by broad spectrum of anomalies, including hyperandrogenemia, insulin resistance, dyslipidemia, body adiposity, low-grade inflammation and increased cardiovascular disease risks. Recently, new polytherapy consisting low-dose flutamide, metformin pioglitazone in combination with an estro-progestagen resulted the regulation endocrine clinical markers young non-obese PCOS women. However, metabolic processes involved this...
Abstract Hereditary breast and ovarian cancer syndrome (HBOC) is partly due to the presence of mutations in BRCA genes. Triple-negative (TN) (BC) shares histological characteristics with germline BRCA1 mutation-associated tumours. We have investigated metabolic profiles human cell lines carrying pathogenic by non-targeted liquid chromatography coupled mass spectrometry technology. Based on our vitro results, we performed a targeted metabolomic analysis plasma samples from TN HBOC patients...
Article2 June 2020Open Access Transparent process Nucleotide depletion reveals the impaired ribosome biogenesis checkpoint as a barrier against DNA damage Joffrey Pelletier Corresponding Author [email protected] orcid.org/0000-0001-9174-044X Laboratory of Cancer Metabolism, ONCOBELL Program, Institut d'Investigació Biomèdica de Bellvitge—IDIBELL, L'Hospitalet Llobregat, Spain Search for more papers by this author Ferran Riaño-Canalias Eugènia Almacellas orcid.org/0000-0002-7726-8430 Caroline...
This study compares the patterns of visceral (VIS) and subcutaneous (SC) adipose tissue (AT)-derived metabolites from non-obese (BMI 24-26 kg/m2) obese subjects > 40 with no major metabolic risk factors other than BMI.SC- VIS- AT obtained (Ob) (NOb) during surgery were incubated to obtain their metabolites. Differences related obesity or anatomical provenances assessed using an untargeted metabolomics approach based on gas chromatography-mass spectrometry.The overall effect metabolite...
Objective Early lifestyle interventions in children with obesity decrease risk of and metabolic disorders during adulthood. This study aimed to identify signatures associated intervention urine samples from prepubertal obesity. Methods Thirty‐four were studied before after a 6‐month program, anthropometric, metabolic, nutritional variables collected. A nuclear magnetic resonance approach was applied obtain the metabolomic profile samples. Partial least squares‐discriminant analysis (PLS‐DA)...
Isotopic-labeling experiments have been valuable to monitor the flux of metabolic reactions in biological systems, which is crucial understand homeostatic alterations with disease. Experimental determination fluxes can be inferred from a characteristic rearrangement stable isotope tracers (e.g., 13C or 15N) that detected by mass spectrometry (MS). Metabolites measured are generally members well-known pathways, and most them using both gas chromatography (GC)-MS liquid (LC)-MS. In here, we...
Thirdhand smoke (THS) is the accumulation of tobacco gases and particles that become embedded in materials. Previous studies concluded THS exposure induces oxidative stress hepatic steatosis liver. Despite knowledge increasing danger exposure, metabolic disorders caused liver are still not well defined. The aim this study to investigate by male mice evaluate effects an antioxidant treatment exposed mice. We investigated from three groups: non-exposed mice, conditions mimic human THS-exposed...
Ectopic fat accumulation in non-adipose tissues is closely related to diabetes-related myocardial dysfunction. Nevertheless, the complete picture of lipid metabolites involved metabolic-related alterations not fully characterized. The aim this study was characterize specific profile hearts an animal model obesity/insulin resistance induced by a high-fat diet (HFD). cardiac lipidome profiles were assessed via liquid chromatography–mass spectrometry (LC–MS)/MS-MS and laser...
Sexual dimorphism influences cardiovascular outcomes in type 1 diabetes (T1D), with women facing a higher relative risk of macrovascular events compared to men, especially after menopause. This study hypothesizes that abnormalities intermediate metabolism may be associated cardiac autonomic neuropathy (CAN) T1D. We aim assess low molecular weight metabolites (LMWM) as markers CAN T1D, considering the effects sexual and age. In this cross-sectional study, we included 323 subjects T1D (147 176...
: Mastitis is one of the main complications during breastfeeding and contributes to cessation breastfeeding. However, etiopathogenesis diagnosis mastitis are complex not yet well defined. We aimed identify metabolic lipidic changes in human milk acute subacute order detect potential biomarkers mastitis.
Abstract Hyperinsulinaemic androgen excess (HIAE) in prepubertal and pubertal girls usually precedes a broader pathological phenotype adulthood that is associated with anovulatory infertility, metabolic syndrome type 2 diabetes. The derangements determine these long-term health risks remain to be clarified. Here we use NMR MS-based metabolomics show serum levels of methionine sulfoxide HIAE are an indicator the degree oxidation methionine-148 residue apolipoprotein-A1. Oxidation apo-A1...