A5042099620- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle metabolism and nutrition
- Metabolism, Diabetes, and Cancer
- Muscle Physiology and Disorders
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and biosynthesis
- RNA modifications and cancer
- Regulation of Appetite and Obesity
- Fatty Acid Research and Health
- Pancreatic function and diabetes
- Polyamine Metabolism and Applications
- Heat shock proteins research
- Body Composition Measurement Techniques
- PARP inhibition in cancer therapy
- Cardiovascular Disease and Adiposity
- RNA regulation and disease
- Metabolism and Genetic Disorders
- Genomics and Chromatin Dynamics
- Sirtuins and Resveratrol in Medicine
Universitat de Barcelona
2017-2024
Institut d'Investigació Biomédica de Bellvitge
2017-2023
Appropriate cristae remodeling is a determinant of mitochondrial function and bioenergetics thus represents crucial process for cellular metabolic adaptations. Here, we show that architecture expression the master cristae-remodeling protein OPA1 in proopiomelanocortin (POMC) neurons, which are key sensors implicated energy balance control, affected by fluctuations nutrient availability. Genetic inactivation POMC neurons causes dramatic alterations topology, Ca2+ handling, reduction...
Metabolic plasticity is the ability of a biological system to adapt its metabolic phenotype different environmental stressors. We used whole-body and tissue-specific phenotypic, functional, proteomic, metabolomic transcriptomic approach systematically assess in diet-induced obese mice after combined nutritional exercise intervention. Although most obesity overnutrition-related pathological features were successfully reverted, we observed high degree dysfunction visceral white adipose tissue,...
Osteocytes, the most abundant of bone cells, differentiate while they remain buried within matrix. This encasement limits their access to nutrients and likely affects differentiation, a process that remains poorly defined. Here, we show restriction in glucose supply promotes osteocyte transcriptional program also being associated with increased mitochondrial DNA levels. Glucose deprivation triggered activation AMPK/PGC-1 pathway. AMPK SIRT1 activators or PGC-1α overexpression are sufficient...
High-resolution respirometry in human permeabilized muscle fibers is extensively used for analysis of mitochondrial adaptions to nutrition and exercise interventions, linked athletic performance. However, the lack standardization experimental conditions limits quantitative inter- intra-laboratory comparisons.
Calorie restriction (CR) exerts remarkable, beneficial effects on glucose homeostasis by mechanisms that are not fully understood. Given the relevance of white adipose tissue (WAT) in homeostasis, we aimed at identifying main cellular processes regulated WAT response to CR a pathologic context obesity. For this, gene-expression profiling study was first conducted mice fed ad libitum or subjected 40% CR. We found gene network related mitochondria most highly upregulated To role increased...
The excessive accumulation and malfunctioning of visceral adipose tissue (VAT) is a major determinant increased risk obesity-related comorbidities. Thus, stratification people living with obesity according to their amount VAT clinical interest. Currently, the most common measurement methods include mathematical formulae based on anthropometric dimensions, often biased by human errors, bio-impedance, image techniques such as X-ray absorptiometry (DXA) analysis, which requires specialized...
Lipids are highly diverse in their composition, properties and distribution different biological entities. We aim to establish the lipidomes of several insulin-sensitive tissues test plasticity when divergent feeding regimens lifestyles imposed. Here, we report a proton nuclear magnetic resonance (
Objective To compare administration of the glucagon-like peptide-1 (GLP-1) analogue, exenatide, versus dietary supplementation with omega-3 fatty acid-rich Calanus oil on obesity-induced alterations in mitochondrial respiration. Methods Six-week-old female C57BL/6JOlaHSD mice were given high fat diet (HFD, 45% energy from fat) for 12 weeks to induce obesity. Thereafter, they divided three groups where one received exenatide (10 μg/kg/day) via subcutaneously implanted mini-osmotic pumps, a...
The prohibitin (PHB)-binding compound fluorizoline as well PHB-downregulation activate the integrated stress response (ISR) in HEK293T and U2OS human cell lines. This activation is denoted by phosphorylation of eIF2α increases ATF4, ATF3, CHOP protein levels. blockage ISR overexpression GRP78, an increase IRE1 activity, indicate presence ER after treatment. inhibition led to increased sensitivity fluorizoline-induced apoptosis, indicating a pro-survival role this pathway treatment these...
Abstract Metabolic plasticity is the ability of a biological system to adapt its metabolic phenotype different environmental stressors. We used whole-body and tissue-specific phenotypic, functional, metabolomic transcriptomic approach systematically assess in diet-induced obese mice after combined nutritional exercise intervention. Although most pathological features were successfully reverted, we observed high degree dysfunction irreversibility visceral white adipose tissue, characterised...