A5078326943- Chronic Lymphocytic Leukemia Research
- Cell death mechanisms and regulation
- Cancer-related Molecular Pathways
- PI3K/AKT/mTOR signaling in cancer
- Metabolism, Diabetes, and Cancer
- Autophagy in Disease and Therapy
- Lymphoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Calcium signaling and nucleotide metabolism
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Inflammatory mediators and NSAID effects
- Acute Lymphoblastic Leukemia research
- RNA regulation and disease
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Nitric Oxide and Endothelin Effects
- Neuroblastoma Research and Treatments
- Cellular transport and secretion
- Cell Image Analysis Techniques
- Cancer Mechanisms and Therapy
- Peroxisome Proliferator-Activated Receptors
- Adenosine and Purinergic Signaling
Universitat de Barcelona
2014-2024
Institut d'Investigació Biomédica de Bellvitge
2014-2024
Lund University
2022
Manufacturas Serviplast (Spain)
2009-2015
Centro Andaluz de Biología Molecular y Medicina Regenerativa
2014
Centro de Investigación del Cáncer
2010
Universidad de Navarra
2010
Consejo Superior de Investigaciones Científicas
2010
Universidad de Salamanca
2010
Bellvitge University Hospital
1991-2007
Abstract Plants undergo two different developmental programs depending on whether they are growing in darkness (skotomorphogenesis) or the presence of light (photomorphogenesis). It has been proposed that latter is default pathway followed by many plants after germination and before seedling emerges from soil. The transition between pathways tightly regulated. conserved COP1-based complex central light-dependent repression photomorphogenesis darkness. Besides this control, hormones such as...
Abstract Free cytoplasmic dopamine may be involved in the genesis of neuronal degeneration Parkinson's disease and other such diseases. We used SH‐SY5Y human neuroblastoma cells to study effect on cell death, activation stress‐induced pathways, expression α‐synuclein, characteristic protein accumulated Lewy bodies. show that 100 500 μM causes a 40% 60% decrease viability, respectively, triggers autophagy after 24 hr exposure, characterized by presence numerous vacuoles with inclusions....
Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor in adults. Despite concerted efforts to improve current therapies develop novel clinical approaches, patient survival remains poor. As such, increasing attention has focused on developing new therapeutic strategies that specifically target apoptotic pathway order treatment responses. Recently, nutlins, small-molecule antagonists of MDM2, have been developed inhibit p53-MDM2 interaction activate p53 signaling...
The effects of supernatant from the bacterial strain Serratia marcescens 2170 (CS‐2170) on viability different haematopoietic cancer cell lines (Jurkat, NSO, HL‐60 and Ramos) nonmalignant cells (NIH‐3T3 MDCK) was studied. We examined whether this cytotoxic effect due to apoptosis, we purified molecule responsible for determined its chemical structure. Using an MTT assay showed a rapid (4 h) decrease in number viable cells. This according fragmentation pattern DNA, Hoechst 33342 staining FACS...
Activation of Ras induces a variety cellular responses depending on the specific effector activated and intensity amplitude this activation. We have previously shown that calmodulin is an essential molecule in down-regulation Ras/Raf/MEK/extracellularly regulated kinase (ERK) pathway cultured fibroblasts due at least part to inhibitory effect Here we show inhibition synergizes with diverse stimuli (epidermal growth factor, platelet-derived bombesin, or fetal bovine serum) induce ERK...
The mitogenic neuropeptides bombesin and vasopressin markedly increased tyrosine serine phosphorylation of multiple substrates in quiescent Swiss 3T3 fibroblasts, including two major bands Mr 90,000 115,000. Tyrosine these proteins was as judged by immunoprecipitation 32Pi-labeled cells immunoblotting unlabeled with monoclonal antiphosphotyrosine antibodies, elution phenyl phosphate, phospho amino acid analysis. Phosphotyrosyl generated did not correspond either apparent molecular weight or...
Streptozotocin diabetic rats showed more than a 4fold increase in blood glucose levels, whereas hepatic glycogen, fructose 2,6-bisphosphate concentration, and 6-phosphofructo-2-kinase activity were decreased.The "total" the "active" (nonphosphorylated) form of enzyme decreased to different extent, resulting fall "active"J"tota1" ratio.Vanadate administration for 2-week period restored altered values without modifying significantly control animals any parameters studied.Glucokinase was...
Transforming growth factor-beta (TGF-beta) induces apoptosis in hepatocytes, being considered a liver tumor suppressor. However, many human hepatocellular carcinoma (HCC) cells escape from its proapoptotic effects, gaining response to this cytokine terms of malignancy. We have recently reported that the induced by TGF-beta hepatocytes requires up-regulation NADPH oxidase NOX4, which mediates reactive oxygen species (ROS) production. TGF-beta-induced NOX4 expression is inhibited antiapoptotic...
Mantle cell lymphoma (MCL) and chronic lymphocytic leukemia (CLL) remain B-cell malignancies with limited therapeutic options. The present study investigates the in vitro vivo effect of phospholipid ether edelfosine (1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine) MCL CLL.Several lines, patient-derived tumor cells, xenografts severe combined immunodeficient mice were used to examine anti-MCL anti-CLL activity edelfosine. Furthermore, we analyzed mechanism action drug biodistribution...
Aspirin and other non‐steroidal anti‐inflammatory drugs induce apoptosis in many cell types. Although the involvement of caspases has been demonstrated, mechanism leading to caspase activation remains unknown. We have studied role mitochondrial pathway aspirin‐induced apoptosis. The apoptotic effect aspirin was analyzed different lines (Jurkat, MOLT‐4, Raji HL‐60) showing induction cytochrome c release 9, 3 8 processing. Furthermore, early not affected by inhibitor Z‐VAD·fmk preceded loss...
Most of the morphologic changes that are observed in apoptotic cells caused by a set cysteine proteases (caspases) activated during this process. In previous works from our group we found treatment rat fetal hepatocytes with transforming growth factor β1 (TGF-β1) is followed cell death. TGF-β1 mediates radical oxygen species (ROS) production precedes bcl-x L down-regulation, loss mitochondrial transmembrane potential, release cytochrome c, and activation caspase-3 (Herrera et al., FASEB J...
HER2/Neu/ERBB2 is a receptor tyrosine kinase overexpressed in approximately 20% of human breast tumors. Truncated or mutant isoforms that show increased oncogenicity compared with the wild-type are found many Here, we report constitutively active ERBB2 sensitizes epithelial cells to agents induce endoplasmic reticulum stress, altering unfolded protein response (UPR) these cells. Deregulation ERK, AKT, and mTOR activities elicited by was involved mediating this differential UPR response,...
B‐chronic lymphocytic leukaemia (B‐CLL) is characterized by the accumulation of long‐lived CD5 + B lymphocytes. The effect mitoxantrone, a topoisomerase II inhibitor, on B‐CLL cells was studied. Treatment for 48 h with mitoxantrone (0.5 μg/ml) induced decrease in cell viability as determined MTT assay. IC 50 calculated three patients 0.7 μg/ml two them and 1.4 third. In all maximum observed 2 μg/ml. An additive cytotoxic when combined fludarabine (5 μg/ml). Mitoxantrone DNA fragmentation...