A5101494370- Adenosine and Purinergic Signaling
- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Pancreatic function and diabetes
- HIV/AIDS drug development and treatment
- Ion Transport and Channel Regulation
- Metabolism and Genetic Disorders
- Pharmacological Effects and Toxicity Studies
- Chronic Lymphocytic Leukemia Research
- Pancreatic and Hepatic Oncology Research
- Renal Transplantation Outcomes and Treatments
- Epigenetics and DNA Methylation
- Pediatric Hepatobiliary Diseases and Treatments
- Neonatal Health and Biochemistry
- Biochemical and Molecular Research
- HIV Research and Treatment
- Edible Oils Quality and Analysis
- Surface Chemistry and Catalysis
- Advanced biosensing and bioanalysis techniques
- Mitochondrial Function and Pathology
- Radiation Therapy and Dosimetry
- Ion channel regulation and function
- Immune Cell Function and Interaction
- Advanced Radiotherapy Techniques
- RNA modifications and cancer
Universitat de Barcelona
2005-2020
Institut de Biomedicina de la Universitat de Barcelona
2006-2020
University of Chile
2018
University of Hohenheim
2013-2015
Instituto de la Grasa
2013-2014
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2006-2012
Centro de Investigación Biomédica en Red
2007-2011
Southwest General Health Center
2010
Southwest Hospital
2010
Hospital Regional Universitario de Málaga
2009
To better understand the role of human equilibrative (hENTs) and concentrative (hCNTs) nucleoside transporters in physiology pharmacology, we investigated regional, cellular, spatial distribution two hCNTs (hCNT1 hCNT2) hENTs (hENT1 hENT2) four tissues. Using situ hybridization immunohistochemical techniques, found that duodenum expressed hCNT1 hCNT2 mRNAs enterocytes hENT1 hENT2 crypt cells. In these cells, hCNT hENT proteins were predominantly localized apical lateral membrane,...
Adenosine monophosphate (AMP)-activated protein kinase (AMPK) is a heterotrimeric complex that senses intracellular energy status and exerts rapid regulation on energy-demanding -consuming metabolic pathways. Although alterations in the adenosine nucleotide pool are traditionally assumed to be consequence of changes metabolism, this study we have addressed question whether extracellular contributes AMPK regulation. In intestinal rat epithelial cell line IEC-6, addition rapidly increases AMP...
To evaluate the mechanisms involved in macrophage proliferation and activation, we studied regulation of nucleoside transport systems. In murine bone marrow-derived macrophages, nucleosides required for DNA RNA synthesis are recruited from extracellular medium. M-CSF induced synthesis, whereas interferon gamma (IFN-gamma) led to blocked proliferation, only synthesis. Macrophages express at least concentrative systems N1 N2 (CNT2 CNT1 genes, respectively) equilibrative es ei (ENT1 ENT2...
Abstract Deoxynucleoside analogs are used in the treatment of a variety solid tumors. Their transport across plasma membrane may determine their cytotoxicity and thus nucleoside transporter (NT) expression patterns be clinical relevance. Lack appropriate antibodies for use paraffin‐embedded biopsies has been bottleneck to undertake high‐throughput analysis NT Here we report characterization 2 new raised against low‐affinity equilibrative NTs, hENT1 hENT2, suitable that purpose. These...
We attempt to identify the plasma membrane transporter involved in uptake of 5′-deoxy-5-fluorouridine (5′-DFUR), an intermediate metabolite capecitabine. This novel oral fluoropyrimidine is used cancer treatments and a direct precursor cytostatic agent 5′-fluorouracil. also examine role 5′-DFUR cytotoxicity. The human concentrative nucleoside (hCNT1) was cloned from fetal liver expressed <i>Xenopus laevis</i> oocytes. two-electrode voltage-clamp technique demonstrate that 5′-DFUR, but not...
Almost all drugs used in anti-human immunodeficiency virus (HIV)-1 and anticancer therapies require membrane proteins to get into the cell develop their proper activity. Nevertheless, little is known regarding expression activity of specific carriers involved uptake these immune cells. Here, we assessed mRNA levels, protein profile, gene families <i>SLC28</i> (coding for concentrative nucleoside transporters, hCNT1–3), <i>SLC29</i> (equilibrative hENT1–2), <i>SLC22</i> (organic cation...
In murine bone marrow macrophages, lipopolysaccharide (LPS) induces apoptosis through the autocrine production of tumor necrosis factor-α (TNF-α), as demonstrated by fact that macrophages from TNF-α receptor I knock-out mice did not undergo early apoptosis. these conditions LPS up-regulated two concentrative high affinity nucleoside transporters here shown to be expressed in transporter (CNT) 1 and 2, a rapid manner is nevertheless consistent with <i>de novo</i> synthesis carrier proteins....
We examined the role of concentrative nucleoside transporter CNT3 in establishment a transepithelial flux natural nucleosides and their pharmacologically active derivatives renal epithelial cell lines. Murine PCT cells grown on transwell dish showed endogenous activity at apical membrane that was responsible for sodium-dependent both purine pyrimidine nucleosides. hCNT3 also identified human kidney its transport tested. To this end, MDCK cells, lacking activity, were genetically engineered...
Abstract Background Nucleoside analogs used in the chemotherapy of solid tumors, such as capecitabine catabolite 5 ′ -deoxy-5-fluorouridine (5 -DFUR) trigger a transcriptomic response that involves aquaglyceroporin aquaporin 3 along with other p53-dependent genes. Here, we examined whether up-regulation (AQP3) mRNA cancer cells treated -DFUR represents collateral effect drug, or conversely, AQP3 participates activity genotoxic agents. Methods The role cell volume increase, cytotoxicity and...
Nucleoside transport systems and their regulation in human B-lymphocytes have been characterized using the cell lines Raji Bare lymphoma syndrome-1 (BLS-1) as experimental models. These cells express at least three different nucleoside follows: a nitrobenzylthioinosine-sensitive equilibrative system of <i>es</i>-type, which appears to be associated with hENT1 expression, two Na<sup>+</sup>-dependent that may correspond N1 recently N5-type, is guanosine-preferring. B activators such phorbol...
The concentrative pyrimidine-preferring nucleoside transporter 1 (hCNT1), cloned from human fetal liver, was expressed in Xenopus laevis oocytes. Using the two-electrode voltage-clamp technique, it is shown that translocation of nucleosides by this generates sodium inward currents. Membrane hyperpolarization (from -50 to -150 mV) did not affect K(0.5) for uridine, although increased transport current approximately 3-fold. Gemcitabine (a pyrimidine nucleoside-derived drug) but fludarabine...
This study describes a novel mechanism of regulation the high-affinity Na+-dependent adenosine transporter (CNT2) via activation A1 receptors (A1R). is mediated by ATP-sensitive K+ (KATP) channels. The CNT2 and A1R are coexpressed in basolateral domain rat hepatocyte plasma membrane colocalized hepatoma cell line FAO. transient increase CNT2-mediated transport activity triggered (−)-N6-(2-phenylisopropyl)adenosine fully inhibited KATP channel blockers mimicked opener. agonist occurs both...
We previously reported that the human Na+/nucleoside transporter pyrimidine-preferring 1 (hCNT1) is electrogenic and transports gemcitabine 5′-deoxy-5-fluorouridine, a precursor of active drug 5-fluorouracil. Nevertheless, complete electrophysiological characterization basic properties hCNT1-mediated translocation has not been performed yet, exact role adenosine in hCNT1 function addressed either. In present work we have used two-electrode voltage clamp technique to investigate transport...