A5067664369- Adenosine and Purinergic Signaling
- Connexins and lens biology
- Pancreatic and Hepatic Oncology Research
- Drug Transport and Resistance Mechanisms
- Toxin Mechanisms and Immunotoxins
- PARP inhibition in cancer therapy
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Bacillus and Francisella bacterial research
- RNA Interference and Gene Delivery
- Amino Acid Enzymes and Metabolism
- Dermatology and Skin Diseases
- Cellular Mechanics and Interactions
- Hair Growth and Disorders
- Biochemical and Molecular Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Renal Transplantation Outcomes and Treatments
- Cell Adhesion Molecules Research
- Advancements in Transdermal Drug Delivery
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Autoimmune and Inflammatory Disorders Research
- Cancer Treatment and Pharmacology
- HIV/AIDS drug development and treatment
- Cannabis and Cannabinoid Research
Universitat de Barcelona
2013-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2012-2024
Institut de Recerca Sant Joan de Déu
2018-2024
Institute for Research in Biomedicine
2008-2024
Instituto de Salud Carlos III
2015-2024
Hospital Sant Joan de Déu Barcelona
2019-2024
Sant Joan de Déu Research Foundation
2018-2024
Centro de Investigación Biomédica en Red
2011-2021
Centre for Biomedical Network Research on Rare Diseases
2011
Barcelona Biomedical Research Park
2011
Since human Nucleoside Transporters (hNTs) were identified by their activity as transport systems, extensive work has been done to fully characterize them at the molecular and physiological level. Many efforts have addressed identification of selectivity for natural substrates nucleoside analogues used treat several diseases. hNTs belong two different gene families, SLC28 SLC29, encoding Concentrative (hCNTs) Equilibrative (hENTs), respectively. hCNTs hENTs are integral membrane proteins,...
Gemcitabine is widely used for pancreatic, lung, and bladder cancer. However, drug resistance against gemcitabine a large obstacle to effective chemotherapy. Nucleoside transporters, nucleoside nucleotide metabolic enzymes, efflux transporters have been reported be involved in resistance. Although most of the resistant factors are supposed related each other, it unclear how one factor can affect other one. In this study, we established gemcitabine-resistant pancreatic cancer cell lines....
Abstract Background Nucleoside analogs used in the chemotherapy of solid tumors, such as capecitabine catabolite 5 ′ -deoxy-5-fluorouridine (5 -DFUR) trigger a transcriptomic response that involves aquaglyceroporin aquaporin 3 along with other p53-dependent genes. Here, we examined whether up-regulation (AQP3) mRNA cancer cells treated -DFUR represents collateral effect drug, or conversely, AQP3 participates activity genotoxic agents. Methods The role cell volume increase, cytotoxicity and...
Abstract Gemcitabine is a nucleoside analogue with anticancer activity. Inside the cell, it sequentially phosphorylated to generate active drug. Phosphorylated analogues have been shown traffic through gap junctions. We investigated participation of junctional intercellular communication (GJIC) as possible mechanism spreading gemcitabine cytotoxicity in pancreatic tumors. Immunohistochemical analysis cancer biopsies revealed increased connexin 26 (Cx26) content but loss connexins 32 (Cx32)...
Several transport alterations have been described in intestinal inflammatory diseases. This is relevant because the primary function of intestine nutrient and mineral absorption. However, analysis transportome as a whole effect commensal bacteria on it not addressed so far. Five healthy 6 Crohn's disease (CD) samples were hybridized to human HT-12 V4 Illumina GeneChip. Results validated by reverse transcription–polymerase chain reaction (RT-PCR) with additional array data. Organ culture...
Nucleoside transporters (NTs) mediate the uptake of nucleosides and nucleobases across plasma membrane, mostly for salvage purposes. The canonical NTs belong to two gene families, SLC29 SLC28. former encode equilibrative nucleoside transporter proteins (ENTs), which facilitative diffusion natural with broad selectivity, whereas latter concentrative (CNTs), are sodium-coupled show high affinity substrates variable selectivity. These expressed in most cell types, exhibiting apparent functional...
Games are excellent teaching tools for self-learning. Students playing a game enjoy themselves and at the same time learn basic important concepts. Different games including crossword puzzles, word search knight’s tour games, connecting dots, mazes, matching two sets, amidakuji, logic were used continuous assessment during confinement due to pandemic. These developed as part of GINDO-UB180 innovation group activities. Since these very successful, we decided combine all them construct new...
Abstract Epithelial‐to‐mesenchymal transition (EMT) is an important pro‐fibrotic event in which tubular epithelial cells are transformed into myofibroblasts. Nucleoside transporters (NT) regulated by many factors and processes, some of involved fibrosis, such as cytokines, inflammation, proliferation. Equilibrative nucleoside transporter 1 (ENT1) has been proved to be the most widely expressed adenosine transporter. In that sense, ENT1 may a key player cell damage signaling. Here we analyze...
Pancreatic ductal adenocarcinoma is a particularly challenging malignancy characterized by poor responsiveness to conventional chemotherapy. Although this tumor frequently overexpresses or possesses constitutively activated variants of IGF-IR and EGFR/Her-2, clinical trials using inhibitors these receptors have failed. ErbB been proposed as one mechanism involved in the resistance inhibitors. Therefore, combined treatment with both would appear be good strategy for overcoming emergence...
Combined therapies play a key role in the fight against complex pathologies, such as cancer and related drug-resistance issues. This is particularly relevant targeted where inhibition of drug target can be overcome by cross-activating complementary pathways. Unfortunately, combinations approved to date –mostly based on small molecules– face several problems toxicity effects, which limit their clinical use. To address these issues, we have designed new class RNase H-sensitive construct (3ASO)...
: YAT2150 is a first-in-class antiplasmodial compound that has been recently proposed as new interesting drug for malaria therapy.
<i>SLC28</i> genes encode three plasma membrane transporter proteins, human concentrative nucleoside (CNT)1, CNT2, and CNT3, all of which are implicated in the uptake natural nucleosides a variety analogs used chemotherapy cancer viral inflammatory diseases. Mechanisms determining their trafficking toward not well known, although this might eventually become target for therapeutic intervention. The regulator RS1, was initially identified as short-term, post-transcriptional high-affinity,...
Follicular targeting has gained more attention in recent decades, due to the possibility of obtaining a depot effect topical administration and its potential as tool treat hair follicle-related diseases. Lipid core ethyl cellulose lipomers were developed optimized, following which characterization their physicochemical properties was carried out. Dexamethasone encapsulated (size, 115 nm; polydispersity, 0.24; zeta-potential (Z-potential), +30 mV) vitro release profiles against dexamethasone...
Dexamethasone-loaded polymer hybrid nanoparticles were developed as a potential tool to treat alopecia areata due their follicular targeting ability. Freeze drying (FD) is common technique used improve nanoparticle stability; however, there are few studies focused on its effect ethyl cellulose lipid-core nanoparticles. Nanoparticles lyophilized with different cryoprotectants. Sucrose was selected because it allowed for good resuspension and provided acceptable physicochemical parameters...
We analyzed the differential gene expression in pancreatic cancer cell line NP-18 upon induction of apoptosis caused by cyclin-dependent kinase inhibition triggered either overexpression tumor suppressor <i>p16<sup>INK4A</sup></i>using an adenoviral construction or incubation with chemical inhibitors, roscovitine olomoucine. Screening was performed using cDNA arrays from Clontech that allowed determination 1,176 genes specifically related cancer. The analysis carried...
// Nerea Urtasun 1, 2 , Clara Boces-Pascual 1 Loreto Boix 2, 3 Jordi Bruix Marçal Pastor-Anglada and Sandra Pérez-Torras Molecular Pharmacology Experimental Therapeutics (MPET), Section Biochemistry Pharmacology, Department of Biomedicine, Institute Biomedicine (IBUB), University Barcelona, Spain Oncology Program, CIBER ehd, National Biomedical Research on Liver Gastrointestinal Diseases, Instituto de Salud Carlos III, Barcelona Clinic Cancer (BCLC) Group, Unit, Hospital Clínic Institut...
Combined therapies play a key role in the fight against complex pathologies, such as cancer and related drug-resistance issues. This is particularly relevant targeted where inhibition of drug target can be overcome by cross-activating complementary pathways. Unfortunately, combinations approved to date –mostly based on small molecules– face several problems toxicity effects, which limit their clinical use. To address these issues, we have designed new class RNase H-sensitive nanostructure...