A5084038246- Malaria Research and Control
- Mosquito-borne diseases and control
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Extracellular vesicles in disease
- Erythrocyte Function and Pathophysiology
- Research on Leishmaniasis Studies
- Vibrio bacteria research studies
- Trypanosoma species research and implications
- Complement system in diseases
- Neonatal Health and Biochemistry
- Pharmacological Effects of Natural Compounds
- Lysosomal Storage Disorders Research
- Genetics, Bioinformatics, and Biomedical Research
- Biological Research and Disease Studies
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Amoebic Infections and Treatments
- Computational Drug Discovery Methods
- Cancer therapeutics and mechanisms
- Plant-based Medicinal Research
- Diverse Scientific Research Studies
- Dendrimers and Hyperbranched Polymers
- Infant Nutrition and Health
- Diabetes and associated disorders
Barcelona Institute for Global Health
2020-2025
Universitat de Barcelona
2020-2025
Institute for Bioengineering of Catalonia
2020-2025
Institut de Nanociència i Nanotecnologia de la Universitat de Barcelona
2020-2024
Max Planck Institute of Colloids and Interfaces
2018-2022
Barcelona Institute for Science and Technology
2022
Center for Research and Advanced Studies of the National Polytechnic Institute
2015-2018
Instituto Politécnico Nacional
2015
Infection with Plasmodium falciparum enhances extracellular vesicle (EV) production in parasitized red blood cells (pRBCs), an important mechanism for parasite-to-parasite communication during the asexual intraerythrocytic life cycle. The e ndosomal s orting c omplex r equired t ransport (ESCRT), and particular ESCRT-III sub-complex, participates formation of EVs higher eukaryotes. However, RBCs have lost majority their organelles through maturation process, including reduction vesicular...
ABSTRACT The problems associated with the drugs currently used to treat leishmaniasis, including resistance, toxicity, and high cost of some formulations, call for urgent identification new therapeutic agents novel modes action. aggregated protein dye YAT2150 has been found be a potent antileishmanial compound, half-maximal inhibitory concentration (IC 50 ) approximately 0.5 µM against promastigote amastigote stages Leishmania infantum . encapsulation in liposomes significantly improved its...
The endosomal sorting complex required for transport (ESCRT) orchestrates cell membrane-remodeling mechanisms in eukaryotes, including endocytosis. However, ESCRT functions phagocytosis(ingestion of ≥ 250 nm particles), has been poorly studied. In macrophages and amoebae, phagocytosis is nutrition attack to other microorganisms cells.In Entamoeba histolytica, the voracious protozoan responsible human amoebiasis, phagocytosisis a land mark virulence. Here, we have investigated role ESCRT-III...
Our understanding of the molecular mechanisms undergirding artemisinin (ART) resistance in Plasmodium falciparum is currently based on two organizing principles: reduced hemoglobin trafficking into digestive food vacuole, resulting lower levels activated ART, and increased tolerance to ART-induced oxidative stress infected erythrocyte. We had previously proposed an extracellular vesicle (EV) export model ART P. falciparum. This predicts that EV abundance will be altered by exposure peptide...
The second-line antileishmanial compound pentamidine is administered intramuscularly or, preferably, by intravenous infusion, with its use limited severe adverse effects, including diabetes, hypoglycemia, myocarditis and renal toxicity. We sought to test the potential of phospholipid vesicles improve patient compliance efficacy this drug for treatment leishmaniasis means aerosol therapy. targeting macrophages pentamidine-loaded liposomes coated chondroitin sulfate or heparin increased about...
Here, we investigated the role of EhVps32 protein (a member endosomal-sorting complex required for transport) in endocytosis Entamoeba histolytica, a professional phagocyte. Confocal microscopy, TEM and cell fractionation revealed cytoplasmic vesicles also located adjacent to plasma membrane. Between 5 30 min phagocytosis, was detected on some erythrocytes-containing phagosomes acidic nature, at 60 it returned appeared images membranous structures vicinity ingested erythrocytes. EhVps32,...
The current decline in antimalarial drug efficacy due to the evolution of resistant Plasmodium strains calls for new strategies capable improving bioavailability antimalarials, especially those whose lipophilic character imparts them a low solubility biological fluids. Here we have designed, synthesized and characterized amphiphilic zwitterionic block copolymers forming nanoparticles penetrating intestinal epithelium that can be used oral administration. Poly(butyl...
Abstract Malaria, caused by different species of protists the genus Plasmodium , remains among most common causes death due to parasitic diseases worldwide, mainly for children aged under 5. One main obstacles malaria eradication is speed with which pathogen evolves resistance drug schemes developed against it. For this reason, it urgent find innovative therapeutic strategies offering sufficient specificity parasite minimize evolution and side effects. In context, nanotechnology‐based...
Malaria is an infectious disease transmitted by mosquitos, whose control hampered drug resistance evolution in the causing agent, protist parasites of genus Plasmodium, as well mosquito to insecticides. New approaches fight this are, therefore, needed. Research into targeted delivery expanding strategy increases treatment efficacies. Alternatively, targeting parasite humans, here we use single-chain polymer nanoparticles (SCNPs) target at ookinete stage, which one stages mosquito. This...
Abstract Background By 2016, signs of emergence Plasmodium falciparum resistance to artemisinin and partner drugs were detected in the Greater Mekong Subregion. Recently, independent evolution has also been reported Africa South America. This alarming scenario calls for urgent development new antimalarials with novel modes action. We investigated interference protein aggregation, which is potentially toxic cell occurs abundantly all stages, as a hitherto unexplored drug target pathogen....
The rampant evolution of resistance in Plasmodium to all existing antimalarial drugs calls for the development improved therapeutic compounds and adequate targeted delivery strategies them. Loading antimalarials nanocarriers specifically parasite will contribute administration lower overall doses, with reduced side effects patient, higher local amounts parasitized cells an increased lethality toward pathogen. Here, we report dendronized hyperbranched polymers (DHPs), capacity loading, that...
A novel family of 4-aminoacridine derivatives was obtained by linking this heteroaromatic core to different trans-cinnamic acids. The 4-(N-cinnamoylbutyl)aminoacridines exhibited in vitro activity the low- or sub-micromolar range against (i) hepatic stages Plasmodium berghei, (ii) erythrocytic forms falciparum, and (iii) early mature gametocytes falciparum. most active compound, having a meta-fluorocinnamoyl group linked acridine core, 20- 120-fold more potent, respectively, gametocyte...
4,9-diaminoacridines with reported antiplasmodial activity were coupled to different trans-cinnamic acids, delivering a new series of conjugates inspired by the covalent bitherapy concept. The compounds more potent than primaquine against hepatic stages Plasmodium berghei, although this was accompanied cytotoxic effects on Huh-7 hepatocytes. Relevantly, displayed nanomolar activities blood stage P. falciparum parasites, no evidence hemolytic below 100 µM. Moreover, at least 25-fold...
: YAT2150 is a first-in-class antiplasmodial compound that has been recently proposed as new interesting drug for malaria therapy.
The methyl erythritol phosphate (MEP) pathway of isoprenoid biosynthesis is essential for malaria parasites and also several human pathogenic bacteria, thus representing an interesting target future antimalarials antibiotics diagnostic strategies. We have developed a DNA aptamer (D10) against Plasmodium falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), the second enzyme this metabolic route. D10 binds in vitro to recombinant DXR from P. Escherichia coli, showing at 10 µM ca....
New biomarkers have to be developed in order increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves use laboratory animals and is time-consuming costly, especially when target Plasmodium , whose variable antigen expression complicates development long-lived biomarkers. To circumvent these obstacles, we applied Systematic Evolution Ligands by EXponential enrichment method identification DNA aptamers against falciparum -infected red blood...
UDP-N-acetylglucosamine (UDP-GlcNAc) is a crucial sugar nucleotide for glycan synthesis in eukaryotes. In the malaria parasite Plasmodium falciparum, UDP-GlcNAc synthesized via hexosamine biosynthetic pathway (HBP) and essential glycosylphosphatidylinositol (GPI) anchor production, most prominent form of protein glycosylation parasite. this study, we explore conditional knockout glucosamine-6-phosphate N-acetyltransferase (PfGNA1), key HBP enzyme. PfGNA1 depletion led to significant...
The endosomal sorting complex required for transport (ESCRT) is a multi-protein machinery involved in several membrane remodeling processes. Different approaches have been used to resolve how ESCRT proteins scission membranes. However, the underlying mechanisms generating deformations are still matter of debate. Here, giant unilamellar vesicles, microfluidic technology, and micropipette aspiration combined continuously follow ESCRT-III-mediated on single-vesicle level first time. With this...