A5082121093- Lipid Membrane Structure and Behavior
- Pickering emulsions and particle stabilization
- Micro and Nano Robotics
- Polymer Surface Interaction Studies
- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- RNA Interference and Gene Delivery
- ATP Synthase and ATPases Research
- Cancer, Hypoxia, and Metabolism
- Nanopore and Nanochannel Transport Studies
- Surfactants and Colloidal Systems
- Mitochondrial Function and Pathology
- Ionic liquids properties and applications
- Polydiacetylene-based materials and applications
- Luminescence and Fluorescent Materials
Max Planck Institute of Colloids and Interfaces
2018-2022
University of Groningen
2021
Effective metabolic pathways are essential for the construction of in vitro systems mimicking biochemical complexity living cells. Such require inclusion a branch that ensures availability reducing equivalents. Here, we built minimal enzymatic pathway confinable lumen liposomes, which redox status nicotinamide cofactors NADH and NADPH is controlled by an externally provided formate. Formic acid permeates membrane where luminal formate dehydrogenase uses NAD+ to form carbon dioxide. Carbon...
Abstract Polymer‐based nanoparticles have an increasing presence in research due to their attractive properties, such as flexible surface functionality design and the ability scale up production. Poly(ionic liquid) (PIL) of size below 50 nm are very unique terms high charge density internal onion‐like morphology. The interaction between PIL giant unilamellar vesicles (GUVs) various densities is investigated. GUVs represent a convenient model system they mimic curvature plasma membranes,...
The interactions between biomembranes and particles are key to many applications, but the lack of controllable model systems study them limits progress in their research. Here, we describe how Janus polystyrene microparticles, half coated with iron, can be partially engulfed by artificial cells, namely giant vesicles, goals control investigate adhesion degree encapsulation. interaction these cell membrane is mediated electrostatic charge, offering a further mode modulation addition iron...
The endosomal sorting complex required for transport (ESCRT) is a multi-protein machinery involved in several membrane remodeling processes. Different approaches have been used to resolve how ESCRT proteins scission membranes. However, the underlying mechanisms generating deformations are still matter of debate. Here, giant unilamellar vesicles, microfluidic technology, and micropipette aspiration combined continuously follow ESCRT-III-mediated on single-vesicle level first time. With this...
The interactions between biomembranes and particles are key to many applications, but the lack of controllable model systems study them limits progress in their research. Here, we describe how Janus polystyrene microparticles, half coated with iron, can be partially engulfed by artificial cells, namely giant vesicles, goals control investigate adhesion degree encapsulation. interaction these cell membrane is mediated electrostatic charge, offering a further mode modulation addition iron...
Abstract The endosomal sorting complex required for transport (ESCRT) is a multi-protein involved in several membrane remodelling processes. Different approaches have been used to dissect the mechanism by which ESCRT proteins produce scission membranes. However, underlying mechanisms generating deformations remain poorly understood. In this study, giant unilamellar vesicles (GUVs), microfluidic technology and micropipette aspiration are combined continuously follow ESCRT-III-mediated on...