A5019973674- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Microbial Inactivation Methods
- Surfactants and Colloidal Systems
- Advanced Polymer Synthesis and Characterization
- Microfluidic and Bio-sensing Technologies
- Molecular Junctions and Nanostructures
- Cellular transport and secretion
- Luminescence and Fluorescent Materials
- Force Microscopy Techniques and Applications
- Analytical Chemistry and Sensors
- ATP Synthase and ATPases Research
- Micro and Nano Robotics
- Quantum Dots Synthesis And Properties
- Supramolecular Self-Assembly in Materials
- Nanoparticle-Based Drug Delivery
- Polymer Surface Interaction Studies
- Pickering emulsions and particle stabilization
- Mitochondrial Function and Pathology
- Spectroscopy and Quantum Chemical Studies
- Membrane Separation and Gas Transport
- Advanced Fluorescence Microscopy Techniques
- Hydrogels: synthesis, properties, applications
University of Groningen
2019-2025
Universidade Federal de Pernambuco
2010-2024
Delft University of Technology
2024
Max Planck Institute of Colloids and Interfaces
2014-2022
Universidade Federal de São Paulo
2014-2022
University of Glasgow
2020
Max Planck Society
2019
Fundação de Apoio à Universidade Federal de São Paulo
2018
Abstract Taking a photo typically requires the object of interest to stand still. In science, imaging is potentiated by optical and electron microscopy. However, living soft matter are not Thus, biological preparations for microscopy usually include fixation step. Similarly, immobilization strategies required or substantially facilitate cells lipid vesicles even more so acquiring high-quality data via fluorescence-based techniques. Here, we describe simple yet efficient method immobilize...
1,6-Diphenyl-1,3,5-hexatriene (DPH) is one of the most commonly used fluorescent probes to study dynamical and structural properties lipid bilayers cellular membranes via measuring steady-state or time-resolved fluorescence anisotropy. In this study, we present a limitation in use DPH predict order acyl chains when bilayer doped with itraconazole (ITZ), an antifungal drug. Our anisotropy measurements showed significant decrease embedded ITZ-containing membrane, suggesting substantial...
Significance Analogous to phospholipids, some polymers assemble into vesicles and can mimic cellular membranes. Apart from enabling compartmentalization in the context of artificial cells, amphiphiles may serve as interface for proteins. However, complex transmembrane proteins were reconstituted with limited success so far. We functionally integrated proton pump bo 3 oxidase (part bacterial respiratory chain) synthetic membranes made PDMS- g -PEO demonstrated lumen acidification. provided...
Resealing of membrane pores is crucial for cell survival. Membrane surface charge and medium composition are studied as defining regulators stability. Pores generated by electric field or detergents. Giant vesicles composed zwitterionic negatively charged lipids mixed at varying ratios subjected to a strong pulse. Interestingly, appear prone catastrophic collapse transforming them into tubular structures. The spectrum destabilization responses includes the generation long-living...
Liposomes represent important drug carrier vehicles in biological systems. A fusogenic liposomal system composed of equimolar mixtures the cationic lipid DOTAP and phospholipid DOPE showed high fusion delivery efficiencies with cells vesicles. However, aspects thermodynamics involving interaction these liposomes biomimetic systems remain unclear. Here, we investigate this large unilamellar vesicles (LUVs) zwitterionic POPC increasing fractions anionic POPG up to 30 mol % cholesterol. The...
Sensing of the biophysical properties membranes using molecular reporters has recently regained widespread attention. This was elicited by development new probes exquisite optical and increased performance, combined with developments in fluorescence detection. Here, we report on lifetime imaging various rigid flexible fluorescent dyes to probe synthetic biological at steady state as well upon action external membrane-modifying agents. We tested solvatochromic Nile red...
Abstract Many drug targets are located in intracellular compartments of cells but they often remain inaccessible to standard imaging and therapeutic agents. To aid delivery, carrier nanoparticles have been used overcome the barrier imposed by plasma membrane. The must entrap large amounts cargo, efficiently quickly deliver cargo cytosol or other compartments, be inert; should not induce cellular responses alter cell state course delivery. This study demonstrates that cationic liposomes with...
Theoretical analysis and experimental quantification on the ellipsoidal relaxation of vesicles are presented. The current work reveals simplicity universal aspects this process. Helfrich formula is shown to apply dynamic moderate-to-high tension membranes, a closed-form solution derived which predicts vesicle aspect ratio as function time. Scattered data unified by time scale, leads similarity behavior, governed distinctive for each type. Two separate regimes in identified, namely,...
Abstract A variety of artificial cells springs from the functionalization liposomes with proteins. However, these models suffer low durability without repair and replenishment mechanisms, which can be partly addressed by replacing lipids polymers. Yet natural membranes are also dynamically remodeled in multiple cellular processes. Here, we show that synthetic amphiphile undergo fusion, mediated protein machinery for synaptic secretion. We integrated fusogenic SNAREs polymer hybrid vesicles...
The use of Quantum Dots (QDs) as fluorescent probes for understanding biological functions has emerged an advantageous alternative over application conventional dyes. Intracellular delivery QDs is currently a specific field research. When are tracking target in live cells, they mostly applied extracellular membrane labeling. In order to study intracellular molecules and structures it necessary deliver free into the cell cytosol. this work, we adapted freeze thaw method encapsulate water...
Liposomes are used in synthetic biology as cell-like compartments and their microfluidic production through double emulsions allows for efficient encapsulation of various components. However, residual oil the membrane remains a critical bottleneck creating pristine phospholipid bilayers. It has been discovered that osmotically driven shrinking leads to detachment drop. Separation inside chip realized automate procedure, which controlled continuous monodisperse liposomes.
Membrane fusion is a ubiquitous process associated with multitude of biological events. Although it has long been appreciated that membrane mechanics plays an important role in fusion, the molecular interplay between and remained elusive. For example, although different lipids modulate differently, depending on their composition, molar ratio, complex interactions, differing lipid compositions may lead to similar mechanical properties. This raises question whether (i) specific composition or...
Abstract Polymer‐based nanoparticles have an increasing presence in research due to their attractive properties, such as flexible surface functionality design and the ability scale up production. Poly(ionic liquid) (PIL) of size below 50 nm are very unique terms high charge density internal onion‐like morphology. The interaction between PIL giant unilamellar vesicles (GUVs) various densities is investigated. GUVs represent a convenient model system they mimic curvature plasma membranes,...
The phase separation in aqueous solutions of a double-hydrophilic block copolymer (DHBC) at concentrations mimicking the crowded environment cells and its performance as potential protein purification system are investigated. Similar to processes self-coacervation, coexistence polymer-rich polymer-depleted microscopic phases is observed partitioning various species them Whereas small molecules, poly(ethylene glycol), dextran can moderately partition both phases, proteins DNA strongly...
Abstract Many drug targets are located in intracellular compartments of cells but they often remain inaccessible to standard imaging and therapeutic agents. To aid delivery, carrier nanoparticles have been used overcome the barrier imposed by plasma membrane. The must entrap large amounts cargo, efficiently quickly deliver cargo cytosol or other compartments, be as inert possible. In words, should not induce cellular responses alter cell state course delivery. Here, we show that cationic...