A5013495860- Lipid Membrane Structure and Behavior
- Spectroscopy and Quantum Chemical Studies
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Surfactants and Colloidal Systems
- Protein Structure and Dynamics
- Molecular Sensors and Ion Detection
- DNA and Nucleic Acid Chemistry
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Cholesterol and Lipid Metabolism
- Advanced Fluorescence Microscopy Techniques
- Luminescence and Fluorescent Materials
- Photosynthetic Processes and Mechanisms
- Nanopore and Nanochannel Transport Studies
- Protein Interaction Studies and Fluorescence Analysis
- Cell death mechanisms and regulation
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nanoparticle-Based Drug Delivery
- Electromagnetic Fields and Biological Effects
- Electrochemical Analysis and Applications
- Antimicrobial Peptides and Activities
- thermodynamics and calorimetric analyses
- bioluminescence and chemiluminescence research
- Photochromic and Fluorescence Chemistry
Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry
2015-2024
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2009-2022
General University Hospital in Prague
2022
Charles University
2022
University of Cambridge
2010-2022
Czech Academy of Sciences
2007-2020
AGH University of Krakow
2015
Saarland University
2009
Czech Academy of Sciences, Institute of Physics
2006
Wrocław University of Science and Technology
2002-2005
Abstract Understanding interactions of calcium with lipid membranes at the molecular level is great importance in light their involvement signaling, association proteins cellular membranes, and membrane fusion. We quantify these detail by employing a combination spectroscopic methods atomistic dynamics simulations. Namely, time-resolved fluorescent spectroscopy vesicles vibrational sum frequency monolayers are used to characterize local binding sites zwitterionic anionic model assemblies,...
Arginine-rich cell-penetrating peptides do not enter cells by directly passing through a lipid membrane; they instead passively vesicles and live inducing membrane multilamellarity fusion. The molecular picture of this penetration mode, which differs qualitatively from the previously proposed direct mechanism, is provided dynamics simulations. kinetics vesicle agglomeration fusion an iconic peptide-nonaarginine-are documented via real-time fluorescence techniques, while induction...
By means of molecular dynamics simulations with an all-atom force field, we investigated the affinities alkali cations and halide anions for dioleoylphosphatidylcholine lipid membrane in aqueous salt solutions. In addition, changes phospholipid lateral diffusion headgroup mobility upon adding NaCl were observed using fluorescence spectroscopy. The revealed that sodium is attracted to region its concentration being maximal vicinity phosphate groups. Potassium cesium, however, do not...
Abstract Fluorescent probes for detecting the physical properties of cellular structures have become valuable tools in life sciences. The fluorescence lifetime molecular rotors can be used to report on variations local packing or viscosity. We a nucleoside linked meso‐substituted BODIPY fluorescent rotor ( dC bdp ) sense changes DNA microenvironment both vitro and living cells. incorporating respond interactions with DNA‐binding proteins lipids by lifetimes range 0.5–2.2 ns. directly...
The biophysical properties of liposome surfaces are critical for interactions between lipid aggregates and macromolecules. Liposomes formed from cationic lipids, commonly used to deliver genes into cells in vitro vivo, an example such a system. We apply the fluorescence solvent relaxation technique study structure dynamics fully hydrated liquid crystalline bilayers composed mixtures dioleoyltrimethylammoniumpropane (DOTAP) neutral dioleoylphosphatidylcholine (DOPC). Using three different...
Physical properties of oxidized phospholipid (OxPL) membranes consisting binary mixtures 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 10 mol % one two OxPLs, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC) or 1-palmitoyl-2-(5′-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), were investigated experimentally computationally. Fluorescence solvent relaxation (SR) fluorescence correlation spectroscopy z-scan (FCS z-scan) show increased headgroup hydration mobility,...
Interactions of different anions with phospholipid membranes in aqueous salt solutions were investigated by molecular dynamics simulations and fluorescence solvent relaxation measurements. Both approaches indicate that the anion-membrane interaction increases size softness anion. Calculations show iodide exhibits a genuine affinity for membrane, which is due to its pairing choline group propensity nonpolar region acyl chains, latter being enhanced polarizable calculations showing number...
1,6-Diphenyl-1,3,5-hexatriene (DPH) is one of the most commonly used fluorescent probes to study dynamical and structural properties lipid bilayers cellular membranes via measuring steady-state or time-resolved fluorescence anisotropy. In this study, we present a limitation in use DPH predict order acyl chains when bilayer doped with itraconazole (ITZ), an antifungal drug. Our anisotropy measurements showed significant decrease embedded ITZ-containing membrane, suggesting substantial...
Lysosome-activated apoptosis represents an alternative method of overcoming tumor resistance compared to traditional forms treatment. Pulsed magnetic fields open a new avenue for controlled and targeted initiation lysosomal permeabilization in cancer cells via mechanical actuation nanomaterials. In this study we used noninvasive tool; namely, benchtop pulsed system, which enabled remote activation liver cells. The system designed platform that can be wide range biomedical applications. We...
Adsorption of metal cations onto a cellular membrane changes its properties, such as interactions with charged moieties or the propensity for fusion. It is, however, unclear whether cells can regulate ion adsorption and related functions via locally adjusting their composition. We employed fluorescence techniques computer simulations to determine how presence cholesterol-a key molecule inducing heterogeneity-affects sodium calcium zwitterionic phosphatidylcholine bilayers. found that...
DNA nanostructures (DNs) can be designed in a controlled and programmable manner, these structures are increasingly used variety of biomedical applications, such as the delivery therapeutic agents. When exposed to biological liquids, most nanomaterials become covered by protein corona, which turn modulates their cellular uptake response they elicit. However, interplay between living cells DNs still not well established. Namely, there very limited studies that assess corona impact on DN...
Absorption and fluorescence spectra of PRODAN (6-propionyl-2-dimethylaminonaphthalene) were studied by means the time-dependent density functional theory algebraic diagrammatic construction method. The influence environment, a phosphatidylcholine lipid bilayer water, was taken into account employing combination quantum chemical calculations with empirical force-field molecular dynamics simulations. Additionally, experimental absorption emission measured in cyclohexane, vesicles. Both planar...
Abstract Specifically designed and functionalized nanoparticles hold great promise for biomedical applications. Yet, the applicability of is critically predetermined by their surface functionalization biodegradability. Here we demonstrate that amino-functionalized polystyrene (PS-NH 2 ), but not amino- or hydroxyl-functionalized silica particles, trigger cell death in hepatocellular carcinoma Huh7 cells. Importantly, biodegradability plays a crucial role regulation essential cellular...
Coexisting liquid ordered (Lo) and disordered (Ld) lipid phases in synthetic plasma membrane-derived vesicles are commonly used to model the heterogeneity of biological membranes, including their putative rafts. However, raft-associated proteins exclusively partition Ld not Lo phase these systems. We believe that difference stems from different microscopic structures rafts at physiological temperature studied room temperature. To probe this structural diversity across temperatures, we...