A5016686757- Lipid Membrane Structure and Behavior
- Nanopore and Nanochannel Transport Studies
- Force Microscopy Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
- Supramolecular Self-Assembly in Materials
- Protein Structure and Dynamics
- thermodynamics and calorimetric analyses
- Diagnosis and Treatment of Venous Diseases
- Nuclear Structure and Function
- Muscle and Compartmental Disorders
- Body Contouring and Surgery
- RNA Interference and Gene Delivery
Czech Academy of Sciences
2024
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2021-2024
Hebrew University of Jerusalem
2020-2023
Coexisting liquid ordered (Lo) and disordered (Ld) lipid phases in synthetic plasma membrane-derived vesicles are commonly used to model the heterogeneity of biological membranes, including their putative rafts. However, raft-associated proteins exclusively partition Ld not Lo phase these systems. We believe that difference stems from different microscopic structures rafts at physiological temperature studied room temperature. To probe this structural diversity across temperatures, we...
Lipid nanodiscs are small synthetic lipid bilayer structures that stabilized in solution by special circumscribing (or scaffolding) proteins or polymers. Because they create native-like environments for transmembrane proteins, have become a powerful tool structural determination of this class systems when combined with cryo-electron microscopy nuclear magnetic resonance. The elastic properties bilayers determine how the environment responds to membrane protein perturbations, and turn...
Lipid nanodiscs are nanometric bilayer patches enveloped by confining structures, commonly composed of membrane scaffolding proteins (MSPs). To resolve the interplay between MSP geometry, lipid confinement, and material properties on nanodisc shape, we apply a continuum elastic theory accounting for bending, tilting, area deformations. The equilibrium shape is then determined minimizing free energy functional. Analytic expressions derived under simplifying assumptions demonstrate that...
Budding relies on membrane asymmetry, including composition, area, and osmotic differences, involves large curvature changes in nanoscale lipid vesicles. So far, the combined impact of asymmetry high curvatures budding has remained unknown. Here, using continuum elastic theory, pathway is detailed under realistic conditions. It shows that less favored smaller vesicles but demixing can significantly reduce its energy barrier yet compositional deviations more than 7% between bud vesicle only...
The coexistence of liquid ordered Lo and disordered Ld phases in synthetic plasma membrane-derived vesicles serves as a model for biomembrane heterogeneity. However, the connection between structures microscopic present at low temperatures tiny "raft" domains biomembranes body temperature is unclear. To study phase structure across temperatures, we performed atomistic molecular dynamics simulations, differential scanning calorimetry, fluorescence spectroscopy on binary ternary lipid...
The coexistence of liquid ordered Lo and disordered Ld phases in synthetic plasma membrane-derived vesicles serves as a model for biomembrane heterogeneity. However, the connection between structures microscopic present at low temperatures tiny "raft" domains biomembranes body temperature is unclear. To study phase structure across temperatures, we performed atomistic molecular dynamics simulations, differential scanning calorimetry, fluorescence spectroscopy on binary ternary lipid...