A5040106693- Lipid Membrane Structure and Behavior
- Ocular Surface and Contact Lens
- Surfactants and Colloidal Systems
- Spectroscopy and Quantum Chemical Studies
- Advanced Drug Delivery Systems
- Glaucoma and retinal disorders
- RNA Interference and Gene Delivery
- Inhalation and Respiratory Drug Delivery
- Neonatal Respiratory Health Research
- Cholesterol and Lipid Metabolism
- Polydiacetylene-based materials and applications
- Advancements in Transdermal Drug Delivery
- Protein Interaction Studies and Fluorescence Analysis
- Receptor Mechanisms and Signaling
- Molecular Sensors and Ion Detection
- Protein Structure and Dynamics
- Photosynthetic Processes and Mechanisms
- Respiratory Support and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Electrochemical sensors and biosensors
- Proteoglycans and glycosaminoglycans research
- Cellular transport and secretion
- Caveolin-1 and cellular processes
- Microbial bioremediation and biosurfactants
Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry
2014-2024
Czech Academy of Sciences
2007-2022
Masaryk University
2008
Wrocław University of Science and Technology
2005-2007
Czech Academy of Sciences, Institute of Physics
2006
Institute of Physics
2005
Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge their physical state and structure. However, mapping viscosity diffusion in complex composition is currently a non-trivial technical challenge. We report fluorescence lifetime spectroscopy imaging (FLIM) meso-substituted BODIPY molecular rotor localised leaflet model various compositions. prepare large giant unilamellar vesicles (LUVs GUVs) containing phosphatidylcholine (PC) lipids...
The biophysical properties of liposome surfaces are critical for interactions between lipid aggregates and macromolecules. Liposomes formed from cationic lipids, commonly used to deliver genes into cells in vitro vivo, an example such a system. We apply the fluorescence solvent relaxation technique study structure dynamics fully hydrated liquid crystalline bilayers composed mixtures dioleoyltrimethylammoniumpropane (DOTAP) neutral dioleoylphosphatidylcholine (DOPC). Using three different...
Abstract Specifically designed and functionalized nanoparticles hold great promise for biomedical applications. Yet, the applicability of is critically predetermined by their surface functionalization biodegradability. Here we demonstrate that amino-functionalized polystyrene (PS-NH 2 ), but not amino- or hydroxyl-functionalized silica particles, trigger cell death in hepatocellular carcinoma Huh7 cells. Importantly, biodegradability plays a crucial role regulation essential cellular...
The tear film lipid layer (TFLL) covers the film, stabilizing it and providing a protective barrier against environment. TFLL is divided into polar non-polar sublayers, but interplay between classes in these sublayers structure-function relationship of remains poorly characterized. This study aims to provide insight function by elucidating interactions lipids at molecular level.Mixed films O-acyl-ω-hydroxy fatty acids (OAHFA) or phospholipids cholesteryl esters (CE) were used as model TFLL....
The lining of the alveoli is covered by pulmonary surfactant, a complex mixture surface-active lipids and proteins that enables efficient gas exchange between inhaled air circulation. Despite decades advancements in study molecular scale behavior surfactant inherent role number different are not fully understood. most important this system SP-B SP-C. Given this, work we performed nonequilibrium all-atom dynamics simulations to interplay SP-C with multicomponent lipid monolayers mimicking...
We claim that (1) cholesterol protects bilayers from disruption caused by lipid oxidation sequestering conical shaped oxidized species such as 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PZPC) away phospholipid, because and the have complementary shapes (2) mixtures of lipids can self-assemble into much like lysolipid–cholesterol mixtures. The evidence for bilayer protection comes molecular dynamics (MD) simulations dynamic light scattering (DLS) measurements. Unimodal size...
Cholesterol renders mammalian cell membranes more compact by reducing the amount of voids in membrane structure. Because this, cholesterol is known to regulate ability prevent permeation water and water-soluble molecules through membranes. Meanwhile, it also that even seemingly tiny modifications chemical structure can lead notable changes properties. The question is, how significantly do these small affect permeability barrier function membranes? In this work, we applied fluorescence...
The tunnel mouths are evolutionally the most variable regions in structures of haloalkane dehalogenases originating from different bacterial species, suggesting their importance for adaptation enzymes to various substrates. We decided monitor dynamics this particular region by means time-resolved fluorescence spectroscopy and molecular dynamic simulations. To label enzyme specifically, we adapted a novel procedure that utilizes coumarin dye containing halide−hydrocarbon linker, which serves...
Translocation of sterols between cellular membrane leaflets is key importance in organization, dynamics, and signaling. We present a novel translocation mechanism that differs unique manner from the established ones. The bobbing identified here demonstrated for tail-oxidized sterols, but expected to be viable any molecule containing two polar centers at opposite sides molecule. renders across lipid possible without change molecular orientation. For provides an exceptionally facile means...
Calmodulin (CaM) is a ubiquitous calcium-sensitive messenger in eukaryotic cells. It was previously shown that CaM possesses an affinity for diverse lipid moieties, including those found on CaM-binding proteins. These facts, together with our observation accumulates membrane-rich protrusions of HeLa cells upon increased cytosolic calcium, motivated us to perform systematic search unmediated interactions model membranes mimicking the leaflet plasma membranes. A range experimental techniques...
Positioning of peptides with respect to membranes is an important parameter for biological and biophysical studies using model systems. Our experiments five different membrane suggest that the time-dependent fluorescence shift (TDFS) Laurdan can help when distinguishing between peripheral integral binding be a useful, novel tool studying impact transmembrane (TMP) on organization under near-physiological conditions. This article focuses LAH4, α-helical peptide high antimicrobial nucleic acid...
Ethylenediaminetetraacetic acid (EDTA) is frequently used in lipid experiments to remove redundant ions, such as Ca2+, from the sample solution. In this work, combining molecular dynamics (MD) simulations and Langmuir monolayer experiments, we show that on top of expected Ca2+ depletion, EDTA anions themselves bind phosphatidylcholine (PC) monolayers. This binding, originating interaction with choline groups PC lipids, leads adsorption at surface concentration-dependent changes pressure...
We introduce and study a multicomponent lipid film mimicking composition of the human lung surfactant. It consists phospholipids with various headgroups tail saturation. Furthermore, it includes cholesterol oxidized lipids. Langmuir trough fluorescence microscopy experiments are combined fully atomistic molecular dynamics simulations. The considered mixtures form complex interfacial films properties modulated by lateral compression. Cholesterol laterally condenses, lipids expand films; both...
Abstract Additives in vaping products, such as flavors, preservatives, or thickening agents, are commonly used to enhance user experience. Among these, Vitamin E acetate (VEA) was initially thought be harmless but has been implicated the primary cause of e-cigarette product use-associated lung injury, a serious disease. In our study, VEA serves proxy for other additives. To explore its harmful effects, we developed an exposure system subject pulmonary surfactant (PSurf) model VEA-rich vapor....