A5039962214- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Immune Cell Function and Interaction
- Lipid Membrane Structure and Behavior
- Cancer Genomics and Diagnostics
- Ion channel regulation and function
- Renal cell carcinoma treatment
- Bioinformatics and Genomic Networks
- Caveolin-1 and cellular processes
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Hematopoietic Stem Cell Transplantation
- Neuroscience and Neuropharmacology Research
- Cholesterol and Lipid Metabolism
- MicroRNA in disease regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Cells and Metastasis
- Vibrio bacteria research studies
- Hedgehog Signaling Pathway Studies
- Metabolism, Diabetes, and Cancer
- Reproductive System and Pregnancy
- Developmental Biology and Gene Regulation
- Cytokine Signaling Pathways and Interactions
- Genetic factors in colorectal cancer
- Aortic aneurysm repair treatments
Charles University
2008-2024
Imperial College London
2020
Czech Academy of Sciences
2007-2014
Czech Academy of Sciences, Institute of Physiology
2007-2014
Although colorectal cancer (CRC) is the third most frequent cause of related death in Europe, clinically relevant biomarkers for therapy guidance and prognosis are insufficiently reliable. Long non-coding RNAs (lncRNAs) over 200 nucleotides long that not translated into proteins but can influence biological processes. There emerging evidence their involvement solid as oncogenes, tumour suppressors or regulators cell proliferation metastasis development. The goal this study was to evaluate...
Background: Beta-adrenergic signalling plays an important role in several cancer-related processes, including angiogenesis. The impact of beta-blocker use on prognosis cancer patients treated with antiangiogenic agents is unclear. aim this study was to evaluate the association between incidental beta-blockers and outcomes metastatic colorectal (mCRC) bevacizumab-based therapy. Methods: Clinical data from 514 mCRC bevacizumab 2005 2019 were analysed retrospectively. progression-free survival...
The aim of our study was to set up a panel for targeted sequencing chemoresistance genes and the main transcription factors driving their expression evaluate predictive prognostic value in breast cancer patients. Coding regulatory regions 509 genes, selected from PharmGKB Phenopedia, were sequenced using massive parallel blood DNA 105 patients testing phase. In total, 18,245 variants identified which 2565 novel (without rs number dbSNP build 150) Variants with major allele frequency over...
Background . Abdominal aortic aneurysms (AAA) are relatively frequent in elderly population, and their ruptures related with high mortality rate. There no actually used laboratory markers predicting the AAA development, course, rupture. MicroRNAs small noncoding molecules involved posttranscriptional gene expression regulation, influencing processes on cell tissue levels, focus due to potential become diagnostic or prognostic various diseases. Methods Tissue samples of patients healthy...
The liquid biopsy has the potential to improve current clinical practice in oncology by providing real-time personalized information about a patient's disease status and response treatment. In this study, we evaluated 161 peripheral blood (PB) samples that were collected around surgical resection from 47 metastatic colorectal cancer (mCRC) patients using High-Definition Single Cell Assay (HDSCA) workflow. conjunction with standard circulating tumor cell (CTC) enumeration, cellular morphology...
Breast cancer is the most frequent in female population worldwide. The role of germline genetic variability cytochromes P450 (CYP) breast prognosis and individualized therapy awaits detailed elucidation. In present study, we used next-generation sequencing to assess associations variants coding regulatory sequences all human CYP genes with response patients neoadjuvant cytotoxic chemotherapy disease-free survival (n = 105). A total 22 prioritized associating a or above evaluation phase were...
The aim of our study was to assess the predictive role primary tumour sidedness (PTS) in patients with metastatic colorectal cancer (mCRC) harbouring wild-type RAS and treated targeted agents.The cohort included 178 first-line chemotherapy plus cetuximab, panitumumab or bevacizumab.We observed longer progression-free survival (PFS) overall (OS) left-sided (L-CRC) compared right-sided tumours (R-CRC) anti-EGFR mAbs (p=0.0033 p=0.0037), while there no difference bevacizumab (p=0.076 p=0.56)....
The anticancer properties of metformin have been suggested in numerous experimental studies and several retrospective clinical show that its use is associated with improved outcome patients cancer. However, limited data are available for metastatic renal cell carcinoma (mRCC) treated targeted therapy. aim this study was to assess the impact on survival mRCC sunitinib or pazopanib.Clinical from 343 pazopanib first line were analyzed. Progression-free (PFS) overall (OS) compared according...
We monitored the radioligand-binding characteristics of thyrotropin-releasing hormone (TRH) receptors, functional activity G(q/11)alpha proteins, and status whole signaling cascade in HEK293 expressing high levels TRH receptors G(11)alpha. Our analyses indicated that disruption plasma membrane microdomains by cholesterol depletion did not markedly influence binding parameters but it altered efficacy signal transduction. The coupling between receptor was assessed agonist-stimulated...
Abstract Testate amoebae (TA) are a group of free-living protozoa, important in ecology and paleoecology. taxonomy is mainly based on the morphological features shell, as examined by means light microscopy or (environmental) scanning electron (SEM/ESEM). We explored potential applications confocal laser (CLSM), two photon excitation (TPEM), phase contrast, differential interference contrast (DIC Nomarski), polarization to visualize TA shells inner structures living cells, which not possible...
Abstract The molecular mechanisms involved in GPCR‐initiated signaling cascades where the two receptors share same cascade, such as thyrotropin‐releasing hormone (TRH) and angiotensin II (ANG II), are still far from being understood. Here, we analyzed hormone‐induced Ca 2+ responses process of desensitization HEK‐293 cells, which express endogenous ANG receptors. These cells were transfected to exogenously high levels TRH (clone E2) or both G 11 α protein E2M11). We observed that...
NKG2D and its ligands, MICA MICB, are known as the key regulators of NK cells. cells first reconstituted after allogeneic hematopoietic stem cell transplantation (HSCT); therefore, it is crucial to understand their role in HSCT outcome. In presented study, we investigated single amino acid changes across exons 2-4 MICB genes, point mutations within gene, which defines type haploblock (HNK/LNK) donors (n = 124), well patients with acute myeloid leukemia 78). our cohort, found that graft from...
Neural stem cells are fundamental to development of the central nervous system (CNS)—as well as its plasticity and regeneration—and represent a potential tool for neuro transplantation therapy research. This study is focused on examination proliferation dynamic fate embryonic neural (eNSCs) under differentiating conditions. In this work, we analyzed eNSCs alone in presence sonic hedgehog (SHH) or triiodothyronine (T3) which play an important role CNS. We found that inhibition SHH pathway...
Killer-immunoglobulin-like receptors (KIRs) are critical natural killer (NK) cell regulators. The expression of KIRs is a dynamic process influenced by many factors. Their ligands-HLA(Human Leukocyte Antigen) class I molecules-are expressed on all nucleated cells that keep NK under control. In hematopoietic stem transplantation (HSCT), play an essential role in relapse protection. the presented pilot study, we characterized inhibitory KIRS (iKIRs), which protect against untoward lysis,...
Background/Aim: The management of patients with clear cell renal carcinoma (ccRCC) includes prognosis assessment based on TNM classification and biochemical markers. This approach stratifies advanced ccRCC into groups favorable, intermediate, poor prognosis. aim the study was to improve estimation using microRNAs involved in pathogenesis ccRCC. Patients Methods: a histologically-verified set matched FFPE tissue samples (normal tissue, primary tumor, metastasis, n=20+20+20). expression 2,549...