A5028628370- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Lipid Membrane Structure and Behavior
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Caveolin-1 and cellular processes
- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- Pancreatic function and diabetes
- Advanced Fluorescence Microscopy Techniques
- Advanced Proteomics Techniques and Applications
- Protein Hydrolysis and Bioactive Peptides
- Sphingolipid Metabolism and Signaling
- Stress Responses and Cortisol
- Bipolar Disorder and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Mesenchymal stem cell research
- Glycosylation and Glycoproteins Research
- S100 Proteins and Annexins
- Metabolism, Diabetes, and Cancer
- Wound Healing and Treatments
- Photosynthetic Processes and Mechanisms
- Erythrocyte Function and Pathophysiology
- Pharmacological Receptor Mechanisms and Effects
- Tryptophan and brain disorders
Czech Academy of Sciences, Institute of Physiology
2014-2023
Czech Academy of Sciences
2011-2022
In this study, we focused on an analysis of biguanides effects mitochondrial enzyme activities, membrane potential and permeability transition pore function. We used phenformin, which is more efficient than metformin, evaluated its effect rat liver mitochondria isolated hepatocytes. contrast to previously published data, found that after a 5 min pre-incubation, dose-dependently inhibits not only complex I but also II IV activity in mitochondria. The enzymes complexes inhibition paralleled by...
Acylcarnitines are important markers in metabolic studies of many diseases, including metabolic, cardiovascular, and neurological disorders. We reviewed analytical methods for analyzing acylcarnitines with respect to the available molecular structural information, technical limitations legacy methods, potential new mass spectrometry-based techniques provide information on metabolite structure. summarized nomenclature based historical common names abbreviations, we propose use systematic...
Principal Findings HEK293 cells stably expressing PTX-insensitive δ-opioid receptor-Gi1α (C351I) fusion protein were homogenized, treated with low concentrations of non-ionic detergent Brij-58 at 0°C and fractionated by flotation in sucrose density gradient. In optimum range (0.025–0.05% w/v), Brij-58-treated, low-density membranes exhibited 2-3-fold higher efficacy DADLE-stimulated, high-affinity [32P]GTPase [35S]GTPγS binding than the same prepared absence detergent. The potency agonist...
This work aimed to test the effect of 7-day exposure rats multifunctional enkephalin analogs LYS739 and LYS744 at doses 3 mg/kg 10 on protein composition rat spleen lymphocytes, brain cortex, hippocampus. Alterations proteome induced by were compared with those elicited morphine. The changes in profiles analyzed label-free quantification (MaxLFQ). Proteomic analysis indicated that treatment caused significant alterations expression levels lymphocytes (45), cortex (31), hippocampus (42)....
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