A5049434819- Rock Mechanics and Modeling
- Seismic Imaging and Inversion Techniques
- Receptor Mechanisms and Signaling
- Seismic Waves and Analysis
- Alzheimer's disease research and treatments
- Geophysical Methods and Applications
- Geotechnical and Geomechanical Engineering
- Neuropeptides and Animal Physiology
- Drilling and Well Engineering
- Advanced Glycation End Products research
- Seismology and Earthquake Studies
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- earthquake and tectonic studies
- Cholinesterase and Neurodegenerative Diseases
- Caveolin-1 and cellular processes
- Mineral Processing and Grinding
- Earthquake Detection and Analysis
- S100 Proteins and Annexins
- High-pressure geophysics and materials
- Landslides and related hazards
- Neurological Disorders and Treatments
- Glycosylation and Glycoproteins Research
- Ion channel regulation and function
- Structural Health Monitoring Techniques
Charles University
2004-2024
Czech Academy of Sciences, Institute of Physiology
2004-2019
Czech Academy of Sciences
2006-2018
Czech Academy of Sciences, Institute of Geology
1985-2013
Czech Academy of Sciences, Institute of Rock Structure and Mechanics
1996-2006
The overproduction of β-amyloid (Aβ) fragments in transgenic APPswe/PS1dE9 mice results formation amyloid deposits the cerebral cortex and hippocampus starting around four months age leading to cognitive impairment much later. We have previously found an transgene-dependent weakening muscarinic receptor-mediated transmission that was not present young (6-10-week-old) animals but preceded both deficits. Now we investigated immediate prolonged vitro effects non-aggregated Aβ(1-42) on coupling...
Abstract Proper determination of agonist efficacy is essential in the assessment selectivity and signalling bias. Agonist a relative term that dependent on system which it measured, especially being receptor expression level. The operational model (OM) functional agonism useful means for using maximal response to ratio potency its equilibrium dissociation constant (K A ) at active state receptor. However, parameter τ inter-dependent two other parameters OM; agonist’s K highest could be...
Apolipoprotein E4 (apoE4) is the most prevalent genetic risk factor for Alzheimer's disease. We utilized apoE4-targeted replacement mice (approved by Tel Aviv University Animal Care Committee) to investigate whether cholinergic dysfunction, which increases during aging and a hallmark of disease, accentuated apoE4. This revealed that levels pre-synaptic marker, vesicular acetylcholine transporter in hippocampus corresponding electrically evoked release acetylcholine, are similar 4-month-old...
Many extracellular signals are at the cell surface received by specific receptors, which upon activation transduce information to appropriate cellular effector molecules via trimeric G proteins. The protein-mediated cascades ultimately lead highly refined regulation of systems such as sensory perception, growth, and hormonal regulation. Transmembrane signaling may be seriously deranged in various pathophysiological conditions. Over last two decades major experimental effort our group has...
Transgenic APPswe/PS1dE9 mice modeling Alzheimer's disease demonstrate ongoing accumulation of β-amyloid fragments resulting in formation amyloid plaques that starts at the age 4-5 months. Buildup is accompanied by impairment muscarinic transmission becomes detectable this age, well before appearance cognitive deficits manifest around 12 We have recently demonstrated long-term feeding trangenic with specific isocaloric fish oil-based diets improves behavioral parameters. Now we report on...
ABSTRACT The purpose of this paper is the comparison P‐wave velocity and anisotropy, measured at different scales under laboratory field conditions. A shallow seismic refraction survey with shot/receiver spacing up to 10 m was carried out on a flat outcrop lhertzolite in southern part Balmuccia massif. Oriented rock samples were also obtained from locality. particular advantage method used possibility measuring any direction controlled Laboratory tests made spherical peridotite samples, 50...
Xanomeline (3-(Hexyloxy)-4-(1-methyl-1,2,5,6-tetrahydropyridin-3-yl)-1,2,5-thiadiazole) is a muscarinic agonist that considered to be functionally selective for the M1/M4 receptor subtypes. Part of xanomeline binding resistant washing. Wash-resistant activates receptors persistently, except M5 subtype. Mutation leucine 6.46 isoleucine at M1 or M4 abolished persistent activation by wash-resistant xanomeline. Reciprocal mutation made it sensitive Lowering membrane cholesterol and mutants wild...
Abstract Background Early-life stress in the form of maternal separation can be associated with alterations offspring neurodevelopment and brain functioning. Here, we aimed to investigate potential impact prolonged on proteomic profiling prefrontal cortex, hippocampus cerebellum juvenile young adult rats. A special attention was devoted proteins involved process cell death redox state maintenance. Methods Long-Evans pups were separated from their mothers for 3 h daily over first weeks life...
Some G protein–coupled receptors might be spacially targetted to discrete domains within the plasma membrane. Here we assessed localization in membrane of epitope-tagged, fluorescent version thyrotropin-releasing hormone receptor (VSV-TRH-R-GFP) expressed HEK293 cells. Our comparison three different methods cell fractionation (detergent extraction, alkaline treatment/sonication and mechanical homogenization) indicated that dominant portion pool was totally solubilized by Triton X-100 its...