A5040396348- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Bone Tissue Engineering Materials
- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- Orthopaedic implants and arthroplasty
- Mesenchymal stem cell research
- Colorectal Cancer Treatments and Studies
- S100 Proteins and Annexins
- Lung Cancer Diagnosis and Treatment
- Titanium Alloys Microstructure and Properties
- Cancer Cells and Metastasis
- Pleural and Pulmonary Diseases
- Medical Imaging and Pathology Studies
- Renal cell carcinoma treatment
- Salivary Gland Tumors Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Galectins and Cancer Biology
Charles University
2015-2024
Nanomaterials are promising in the development of innovative therapeutic options that include tissue and organ replacement, as well bone repair regeneration. The expansion new nanoscaled biomaterials is based on progress field nanotechnologies, material sciences, biomedicine. In recent decades, nanomaterial systems have bridged line between synthetic natural worlds, leading to emergence a science called design for biological applications. replicating properties providing unique functions...
Colorectal cancer (CRC) ranks among the most common cancers worldwide. Surgical removal remains best strategy for treatment of resectable tumors. An important part caring patients after surgery is monitoring early detection a possible relapse disease. Efforts are being made to improve sensitivity and specificity routinely used carcinoembryonic antigen (CEA) with use additional biomarkers such as microRNAs. The aim our study was evaluate prognostic potential microRNAs their markers disease...
In the case of cancer, death is usually not due to primary tumor itself but dissemination. Analysis circulating cells (CTCs), i.e., responsible for a formation metastases, should provide information useful management cancer patients, fulfilling objectives predictive, preventive, and personalized medicine (PPPM). Despite promising results, decisions on stage disease how guide adjuvant treatment still do include results CTC assessment. We want describe two major reasons why recent diagnostic...
Background and Objectives Utilisation of the one‐step nucleic acid amplification (OSNA) molecular biology method for detection metastatic involvement sentinel lymph nodes (SLNs) in endometrial cancer (EC) patients. A comparison with histopathological ultrastaging a description clinical consequences. Methods Surgically treated EC patients underwent SLNs. Nodes greater than 5 mm were cut into sections 2‐mm thick parallel to short axis node. Odd examined according OSNA method, while even ones...
This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing–thawing method. We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, hemolytic test. The results showed that all types scaffolds should be safe for use in the human body. culturing osteoblast-like cells MG-63 their proliferation better adhesion due presence HA confirmed depending on...
Breast cancer belongs to the most commonly diagnosed malignancies worldwide, with its increasing incidence paralleled by advances in early diagnostics and effective treatments resulting significantly improved survival rates. However, breast survivors often experience reduced quality of life linked long-term health burden as a consequence aggressive oncological applied. Their frequently recorded complains include chronic fatigue, physical activity, disordered sleep, chronification pain,...
Background/Aim: Current research of prostate cancer (PCa) offers a promising way identifying patients with adverse prognosis who do benefit from radical treatment that can affect quality life as resections are associated numerous side-effects. The aim our study was to evaluate the relationship TMPRSS2-ERG fusion gene status, tumor tissue prostate-specific antigen (PSA), 3 (PCA3), miR-23b, miR-26a and miR-221 expression levels in combination preoperative serum PSA level risk PCa recurrence...
Bone tissue engineering tries to simulate natural behavior of hard tissues. This study aimed produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by situ synthesis physical mixing pre-prepared HAp. In resulted calcium deficient form HAp lower crystallinity. The proliferation human osteoblast-like cells MG-63 proved be better the synthesized compared those physically mixed For PVA/HA/HAp ratio 3:1:2, there...
Nanostructured titanium has become a useful material for biomedical applications such as dental implants. Certain surface properties (grain size, roughness, wettability) are highly expected to promote cell adhesion and osseointegration. The aim of this study was compare the biocompatibilities several materials using human osteoblast line hFOB 1.19. Eight different types specimens were examined: machined commercially pure grade 2 (cpTi2) 4 (cpTi4) titanium, nanostructured same grades (nTi2,...
MicroRNAs have the potential to become valuable predictive markers for gastric cancer. Samples of biopsy tissue, routinely taken from cancer patients undergoing palliative chemotherapy, constitute suitable material microRNA profiling with aim predicting effect chemotherapy. Our study group consisted 54 patients, all whom underwent chemotherapy based on 5-fluorouracil (5-FU) or 5-FU in combination platinum derivatives between 2000 and 2013. The expression 29 selected microRNAs genes BRCA1,...
Background Detection of tumor cells in lymph nodes (LNs) removed during the treatment pulmonary by radical surgery is limited possibilities standard histopathological methods. The goal this study was to obtain more accurate pTNM status a sensitive detection micrometastases LNs. Methods A total 885 LNs, an average 13.8 LNs per patient, were 64 surgeries. from same zone pooled together as group, five groups examined each patient. 320 examined. One‐step nucleic acid amplification (OSNA) method...
Neurotransplantation has great potential for future treatments of various neurodegenerative disorders. Preclinically, the Lurcher mutant mouse represents an appropriate model genetically-determined olivocerebellar degeneration. The aim present study was to assess survival naïve and neurally differentiated P19 carcinoma stem cells following transplantation into cerebellum mice wild type littermates.Adult normal (n=51) (n=87) B6CBA strain were used. mean age animals at time 261.5 days....