A5075657665- Reproductive Biology and Fertility
- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Estrogen and related hormone effects
- Birth, Development, and Health
- Endometriosis Research and Treatment
- Human Health and Disease
- Growth Hormone and Insulin-like Growth Factors
- Gynecological conditions and treatments
- Sexual Differentiation and Disorders
- Menstrual Health and Disorders
- Reproductive Physiology in Livestock
- Cervical Cancer and HPV Research
- Hormonal and reproductive studies
- Uterine Myomas and Treatments
- Reproductive Health and Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- Systemic Sclerosis and Related Diseases
- Stress Responses and Cortisol
- Medical and Biological Sciences
- Urticaria and Related Conditions
- Reproductive Health and Contraception
- Education, Psychology, and Social Research
Charles University
2015-2025
University Hospital Plzen
2019-2024
Magna International (Germany)
2023
Royal Tropical Institute
2023
Fundación Instituto Valenciano de Oncología
2021
Pilsen Tools (Czechia)
2012
Henry M. Jackson Foundation
2006
Walter Reed Army Institute of Research
2006
Ústav pro Péči o Matku a Dítě
1985-1995
Czech Academy of Sciences, Institute of Macromolecular Chemistry
1990
BackgroundSENTIX (ENGOT-CX2/CEEGOG-CX1) is an international, multicentre, prospective observational trial evaluating sentinel lymph node (SLN) biopsy without pelvic dissection in patients with early-stage cervical cancer. We report the final preplanned analysis of secondary end-points: SLN mapping and outcomes intraoperative pathology.MethodsForty-seven sites (18 countries) experience participated SENTIX. preregistered stage IA1/lymphovascular space invasion-positive to IB2 (4 cm or smaller...
<h3>Background</h3> Adjuvanticity, which is the ability of neoplastic cells to deliver danger signals, critical for host immune system mount spontaneous and therapy-driven anticancer responses. One such signals<i>,</i> i.e., exposure calreticulin (CALR) on membrane malignant experiencing endoplasmic reticulum (ER) stress, well known its role in activation responses dying cancer cells. However, potential impact CALR contexture primary metastatic high-grade serous carcinomas (HGSCs) prognostic...
<h3>Introduction/Background</h3> The SENTIX was an international, multicentre, prospective single-arm study on sentinel lymph node (SLN) biopsy without systematic pelvic lymphadenectomy in patients with early-stage cervical cancer. Here, we report the final survival outcomes of study. <h3>Methodology</h3> Forty-seven sites across 18 countries participated Patients stages 1A1/LVSI+ - 1B2 (FIGO 2018), common histological types and no preoperatively suspicious lymph-nodes (LN) were...
This is a multicentre, European, prospective trial evaluating the diagnostic accuracy of One Step Nucleic Acid Amplification (OSNA) compared to sentinel lymph nodes histopathological ultrastaging in endometrial cancer patients. Centres with expertise node mapping patients Europe will be invited participate study. Participating units trained on correct usage OSNA RD-210 analyser and nucleic acid amplification reagent kit LYNOAMP CK19 E for rapid detection metastatic nodal involvement, based...
Abstract Purpose We aimed to find predictive tumour characteristics as detected by interim positron-emission tomography/magnetic resonance imaging (PET/MRI) in cervical cancer patients. also investigated the type of response. Furthermore, we compared parameters with disease-free (DFS) and overall survival (OS) outcomes. Methods evaluated 108 patients treated between August 2015 January 2023 external-beam radiotherapy (EBRT) image-guided adaptive brachytherapy (IGABT) who had undergone...
Serum osteocalcin levels peaked 1 yr after oophorectomy in a prospective study of 12 women. Estrogen treatment restored serum to the normal range within 4 months therapy. The changes preceded those bone alkaline phosphatase activity by 1–2 months, these oophorectomized patients and during estrogen treatment. two markers formation over time were significantly different from urinary hydroxyproline excretion. A significant positive correlation was found between 18 with primary...
The aim of our study was to clarify whether the CD44 adhesion molecule as a cancer stem cell marker could also serve prognostic factor in patients with epithelial ovarian (EOC). A retrospective performed on 87 histologically verified EOC. Specimens both primary tumour and implantation metastases were tested from 48 them. expression detected by immunohistochemistry. We looked for cut-off levels using Cox regression model. confirmed statistically significant factors overall survival (OS)...
Background and Objectives Utilisation of the one‐step nucleic acid amplification (OSNA) molecular biology method for detection metastatic involvement sentinel lymph nodes (SLNs) in endometrial cancer (EC) patients. A comparison with histopathological ultrastaging a description clinical consequences. Methods Surgically treated EC patients underwent SLNs. Nodes greater than 5 mm were cut into sections 2‐mm thick parallel to short axis node. Odd examined according OSNA method, while even ones...
The Guidelines for Clinical Practice carriers of pathogenic variants in clinically relevant cancer predisposition genes define the steps primary and secondary prevention that should be provided to these individuals at high risk developing hereditary Czech Republic. drafting guidelines was organized by Oncogenetics Working Group Society Medical Genetics Genomics J. E. Purkyně (SLG ČLS JEP) cooperation with representatives oncology oncogynecology. are based on current recommendations National...
Background: To prospectively assess LLL incidence among cervical cancer patients treated by uterine surgery complemented SLN biopsy, without PLND. Methods: A prospective study in 150 with stage IA1–IB2 bilateral biopsy. Objective assessments, based on limb volume increase (LVI) between pre- and postoperative measurements, subjective patient-perceived swelling were conducted six-month periods over 24-months post-surgery. Results: The cumulative of at 24 months was 17.3% for mild (LVI 10–19%),...
Molecular classification of endometrial carcinomas (EC) divides these neoplasms into four distinct subgroups defined by a molecular background. Given its proven clinical significance, genetic examination is becoming an integral component the diagnostic procedure. Recommended algorithms comprise testing POLE gene, whereas remaining parameters are examined solely immunohistochemistry. The aim this study to share our experiences with EC, which has been conducted using immunohistochemistry and...
Morphological and sexual differentiation extending far into postnatal life in the rat, a species with short gestation, is influenced female first by absence later presence of ovarian oestrogens. Therefore, present study total oestrogen concentration was determined blood normal rats from early critical period hypothalamic until maturity. Wistar aged 5–7 days were used. The animals given heparin s.c. (5- 10-day-old receiving 500 i.u., all others 1000 i.u.) killed decapitation 45 min. later. At...
Rats do not respond to human chorionic gonadotrophin (HCG) in the first days of life: oestrogen is apparently secreted after treatment with HCG until animals are 8–9 old (Freed & Soskin, 1937; Falck, 1953). Similar results have been obtained our colony Wistar rats (Divila, Presl, Krabec Horský, 1971). An attempt was made study relationship age-dependent stage rat ovarian development and refractory condition ovary beginning its responsiveness HCG, by means an autoradiographic technique...