A5020808542- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Vestibular and auditory disorders
- Nerve injury and regeneration
- Electromagnetic Fields and Biological Effects
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Anesthesia and Neurotoxicity Research
- Biofield Effects and Biophysics
- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Neuroscience of respiration and sleep
- Spaceflight effects on biology
- Pluripotent Stem Cells Research
- Neurological disorders and treatments
- Trace Elements in Health
- Chemical Reactions and Isotopes
- Photoreceptor and optogenetics research
- Hemispheric Asymmetry in Neuroscience
- Ophthalmology and Eye Disorders
- Neonatal and fetal brain pathology
- Neuroscience, Education and Cognitive Function
- Muscle Physiology and Disorders
Charles University
2011-2021
Heidelberg University
2002
Adult heterozygous Lurcher mice show a degeneration of almost all Purkinje cells and 90% the granular cerebellum, resulting in ataxia or general deficits motor coordination. These are therefore an excellent model for studying role cerebellar cortex performance, including acquisition new abilities. The performance 3-month-old was studied various behavioural (fall, horizontal bar, rotating cylinder, ladder), spatial orientation (water maze) associative learning (eyelid classical conditioning)...
Young adult heterozygous Lurcher mice constitute an excellent model for studying the role of cerebellar cortex in motor performance—including acquisition new abilities—because early postnatal degeneration almost all their Purkinje and granular cells. Wild-type were classically conditioned eyelid responses using a delay paradigm with or without electrolytic lesion interpositus nucleus. Although late component electrically evoked blink reflexes was smaller amplitude had longer latency than...
The cerebellum is not only essential for motor coordination but also involved in cognitive and affective processes. These functions of the mechanisms their disorders cerebellar injury are completely understood. There a wide spectrum mutant mice which used as models hereditary degenerations. Nevertheless, they differ pathogenesis manifestation particular mutation strain background. aim this work was to compare spatial navigation, learning, memory pcd Lurcher mice, two most frequently mutants....
Abstract Lurcher mutant mice represent a model of olivocerebellar degeneration. They suffer from complete loss Purkinje cells and reduction granule inferior olive neurons. Their wild type littermates serve as healthy controls. The aim the work was to compare solid embryonic cerebellar graft survival within period 9 weeks after their transplantation in adult B6CBA strain. grafts were injected through hole occipital bone. Host sacrificed 3, 6, or cerebella brain‐stems examined histologically...
Neurotransplantation has great potential for future treatments of various neurodegenerative disorders. Preclinically, the Lurcher mutant mouse represents an appropriate model genetically-determined olivocerebellar degeneration. The aim present study was to assess survival naïve and neurally differentiated P19 carcinoma stem cells following transplantation into cerebellum mice wild type littermates.Adult normal (n=51) (n=87) B6CBA strain were used. mean age animals at time 261.5 days....
Abstract Classical eyeblink conditioning is an experimental model widely used for the study of neuronal mechanisms underlying acquisition new motor and cognitive skills. There are two principal interpretations role cerebellum in learning eyelid conditioned responses (CRs). One considers that place where this acquired stored, while second suggests mostly involved proper performance CRs, implying there must be other brain areas process. We checked timing cerebellar interpositus nucleus (IPN)...
Lurcher mutant mice represent a natural model of genetically-determined olivocerebellar degeneration caused by mutation in the δ2 glutamate receptor gene. They suffer from progressive postnatal loss cerebellar Purkinje cells and decrease granule inferior olive neurons. Their wild type littermates serve as healthy controls. A confocal laser scanning microscope was used aiming investigation dynamics changes cortex derived two strains during period 8-21 days. Fluorescent double-staining to...
Evaluation of the direct registration brain cortical and hippocampal activity during a high-frequency electromagnetic field (HF-EMF) exposure was performed. Experimental procedures were done under general anesthesia (urethane, 20%, 2g/kg i.p.) in Lurcher mutant mice, wild type (healthy littermates) used as controls. Animals exposed to HF-EMF with frequency corresponding cellular phones (900 MHz). We gel electrodes (silicon tubes or glass microcapillary filled agar) where connection classical...
Mouse embryonic carcinoma cells (P19 line) were studied for both their survival and developmental potential in the intact cerebellum of B6CBA mice. The P19 cultured labelled with green fluorescent protein using transfection. Cells used transplantation either undifferentiated stage or after 3 days neurodifferentiation induced by retinoic acid. intracerebellar application was performed 43 mice: group A (N = 21) received neuroprogenitors B 22) cells. morphology transplanted within context...
<i>Objective:</i> The nervous, endocrine and immune systems are functionally interconnected so that they may form a complex metasystem. Abnormalities within the neuroendocrine compartment can thus affect mechanisms vice versa. Lurcher-type mutation in mice has profound impact on brain development both homozygous heterozygous individuals, which is followed by system changes. We investigated whether macroscopic changes thymus size were associated with an altered thymocyte or...