A5090302985- Steroid Chemistry and Biochemistry
- Estrogen and related hormone effects
- Organic Chemistry Cycloaddition Reactions
- Fibroblast Growth Factor Research
- Connective tissue disorders research
- Cleft Lip and Palate Research
- Bone and Dental Protein Studies
- Pluripotent Stem Cells Research
- Olfactory and Sensory Function Studies
- Cytokine Signaling Pathways and Interactions
- Craniofacial Disorders and Treatments
- Cancer-related gene regulation
- Mass Spectrometry Techniques and Applications
- Neurogenetic and Muscular Disorders Research
- Analytical Chemistry and Chromatography
- Hedgehog Signaling Pathway Studies
- Neurogenesis and neuroplasticity mechanisms
- Macrophage Migration Inhibitory Factor
- GDF15 and Related Biomarkers
- Developmental Biology and Gene Regulation
- Proteoglycans and glycosaminoglycans research
- dental development and anomalies
- Biochemical Analysis and Sensing Techniques
- Chemokine receptors and signaling
- Protein Tyrosine Phosphatases
University of Michigan
2018-2022
Michigan United
2020
Masaryk University
2010-2019
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2015-2017
University of Veterinary Sciences Brno
2015-2016
University of British Columbia
2015
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2009
Institute of Rheumatology
2005
N.D. Zelinsky Institute of Organic Chemistry
1971-2002
Russian Academy of Sciences
1988-1991
Receptor tyrosine kinase signaling cooperates with WNT/β-catenin in regulating many biological processes, but the mechanisms of their interaction remain poorly defined. We describe a potent activation by FGFR2, FGFR3, EGFR and TRKA kinases, which is independent PI3K/AKT pathway. Instead, this phenotype depends on ERK MAP kinase-mediated phosphorylation WNT co-receptor LRP6 at Ser1490 Thr1572 during its Golgi network-based maturation process. This dramatically increases cellular response to...
Activating mutations in the fibroblast growth factor receptor 3 (FGFR3) cause most common genetic form of human dwarfism, achondroplasia (ACH). Small chemical inhibitors FGFR tyrosine kinase activity are considered to be viable option for treating ACH, but little experimental evidence supports this claim. We evaluated five (TKIs) (SU5402, PD173074, AZD1480, AZD4547 and BGJ398) their against signaling chondrocytes. All TKIs strongly inhibited activation cultured chondrocytes limb rudiment...
The short rib polydactyly syndromes (SRPS) are a group of recessively inherited, perinatal-lethal skeletal disorders primarily characterized by ribs, shortened long bones, varying types and concomitant visceral abnormalities. Mutations in several genes affecting cilia function cause SRPS, revealing role for development. To identify additional SRPS discover novel ciliary molecules required normal skeletogenesis, we performed exome sequencing cohort patients identified homozygosity missense...
Highlights•Sucrose consumption showed specific and selective effects on the peripheral taste system•Dietary sugar lowered chorda tympany responses to sucrose number of PLCβ2+ cells•Responses other sensory qualities modalities were largely unaffected•We observed that phenotypes restored after was removed from dietSummaryElevated is associated with an increased risk for metabolic diseases. Whereas evidence humans, rodents, insects suggests dietary modifies sweet sensation, understanding nerve...
The successional dental lamina (SDL) plays an essential role in the development of replacement teeth diphyodont and polyphyodont animals. A morphologically similar structure, rudimental (RSDL), has been described monophyodont (only one tooth generation) lizards on lingual side developing functional tooth. This rudimentary regresses, which proposed to play a preventing formation future generations teeth. structure reported associated with first molar mouse, we show that this is common all...
The blocking of specific protein-protein interactions using nanoparticles is an emerging alternative to small molecule-based therapeutic interventions. However, the designed as “artificial proteins” generally require modification their surface with (bio)organic molecules and/or polymers ensure selectivity and specificity action. Here, we show that nanosized diamond crystals (nanodiamonds, NDs) without any synthetically installed interface enable efficient targeting family extracellular...
The fungiform papilla (FP) is a gustatory and somatosensory structure incorporating chorda tympani (CT) nerve fibers that innervate taste buds (TB) also contain endings for touch temperature. Hedgehog (HH) pathway inhibition eliminates TB, but CT innervation remains in the FP. Importantly, after HH inhibition, neurophysiological responses to stimuli are eliminated, tactile remain. To examine respond absence of we used Phox2b-Cre; Rosa26LSL-TdTomato reporter mice selectively label with...
Abstract Retinoic acid (RA) is able to induce the differentiation of embryonic stem cells into neuronal lineages. The mechanism this effect unknown but it has been evidenced be dependent on formation floating spheroids called embryoid bodies. Results presented here show that inhibition phosphoinositide 3‐kinase signaling pre‐determines mouse RA induced neurogenesis in monolayer culture with no need bodies formation. J. Cell. Biochem. 113: 563–570, 2012. © 2011 Wiley Periodicals, Inc.
Lineage tracing has shown that most of the facial skeleton is derived from cranial neural crest cells. However, local signals influence postmigratory, crest-derived mesenchyme also play a major role in patterning skeleton. Here, we study BMP signaling regulating fate chondro-osteoprogenitor cells face.A single Noggin-soaked bead inserted into stage 15 chicken embryos induced an ectopic cartilage resembling interorbital septum within palate and other midline structures. In contrast, same...
The leukaemia inhibitory factor is a cytokine that exhibits pleiotropic activities in wide range of cell types. There are evidences factor-regulated signalling pathways involved cardiomyogesis and maintenance cardiomyocytes. In the present work we studied effect on cardiomyogenesis embryonic stem cells together with role serum-born factors. We showed had an during both induction progression phases cells. factor-mediated inhibition was abolished by inhibitors STAT3 activity. These results...
Mouse embryonic carcinoma cells (P19 line) were studied for both their survival and developmental potential in the intact cerebellum of B6CBA mice. The P19 cultured labelled with green fluorescent protein using transfection. Cells used transplantation either undifferentiated stage or after 3 days neurodifferentiation induced by retinoic acid. intracerebellar application was performed 43 mice: group A (N = 21) received neuroprogenitors B 22) cells. morphology transplanted within context...
Craniosynostosis is the premature fusion of cranial bones. The goal this study was to determine if delivery recombinant tissue nonspecific alkaline phosphatase (TNAP) could prevent or diminish severity craniosynostosis in a C57BL/6 FGFR2C342Y/+ model neonatal onset BALB/c postnatal craniosynostosis. Mice were injected with lentivirus encoding mineral targeted form TNAP immediately after birth. Cranial bone as well volume, content and density assessed by micro CT. Craniofacial shape measured...
Chaperonins have classically been thought of as intracellular molecules involved in the correct folding proteins.Their expression is upregulated during times stress such heat (hence their common nomenclature shock proteins [HSP]), anoxia, hypoglycaemia and reactive oxygen species [1].These are conditions found infected tissues or with chronic inflammation rheumatoid synovium.In location they protect cell from apoptotic death due to stress.Increasingly chaperonins recognised subserve...