A5081146584- dental development and anomalies
- Cell death mechanisms and regulation
- Bone Metabolism and Diseases
- Oral and Maxillofacial Pathology
- Bone and Dental Protein Studies
- Bone health and treatments
- RNA Interference and Gene Delivery
- Phagocytosis and Immune Regulation
- interferon and immune responses
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Dental Trauma and Treatments
- Cleft Lip and Palate Research
- NF-κB Signaling Pathways
- Autophagy in Disease and Therapy
- Hair Growth and Disorders
- Osteoarthritis Treatment and Mechanisms
- Pluripotent Stem Cells Research
- Calpain Protease Function and Regulation
- Periodontal Regeneration and Treatments
- Trace Elements in Health
- TGF-β signaling in diseases
- Immune Response and Inflammation
- Dermatologic Treatments and Research
- Cytokine Signaling Pathways and Interactions
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2015-2024
University of Veterinary Sciences Brno
2015-2024
Institute of Animal Physiology of the Slovak Academy of Sciences
2014-2023
Czech Academy of Sciences, Institute of Physiology
2005-2021
Czech Academy of Sciences
2004-2021
Moravian Museum
2015
University Hospital Brno
2011
Guy's Hospital
2004-2009
UCL Biomedical Research Centre
2009
King's College London
2004-2009
The first mouse molar (M1) is the most common model for odontogenesis, with research particularly focused on prenatal development. However, functional dentition forms postnatally, when histogenesis and morphogenesis of tooth completed, roots form physically anchors into jaw. In this work, M1 was studied from birth to eruption, assessing morphogenesis, proliferation apoptosis, correlating these remodeling surrounding bony tissue. completed crown formation between postnatal (P) days 0-2,...
Functional tooth germs in mammals, reptiles, and chondrichthyans are initiated from a dental lamina. The longevity of the lamina plays role governing number generations. Monophyodont species have no replacement lamina, while polyphyodont permanent continuous In diphyodont species, fragments regresses after initiation second generation. Regression seems to be an important mechanism preventing further development teeth. Defects complete removal lead cyst formation has been linked...
This study highlights the dynamic nature of mesenchymal cells during tooth development from bud to bell stage. Condensing cells, labelled on either side developing bud, move toward presumptive roots forming an arc under dental papilla. These take part in formation follicle, which contributes both and its surrounding periodontium, including supporting alveolar bone. study, thus, physically links with tissue into it develops. The results obtained clearly indicate that organ is entity...
Caspase-3 and -7 are generally known for their central role in the execution of apoptosis. However, function is not limited to apoptosis under specific conditions activation has been linked proliferation or differentiation specialised cell types. In present study, we followed localisation activated form caspase-7 during intramembranous (alveolar mandibular bones) endochondral (long bones limbs) ossification mice. both bone types, was detected from beginning embryonic development persisted...
Three-dimensional (3D) cell cultures are to date the gold standard in biomedical research fields due their enhanced biological functions compared conventional two-dimensional (2D) cultures. 3D spheroids, as well organoids, better suited replicate tissue functions, which enables use both vitro models for basic and toxicology, building blocks used tissue/organ biofabrication approaches. Culturing spheroids from bone-derived cells is an emerging technology disease modelling drug screening...
Abstract Recent research indicates that control of cusp morphology involves a signalling center at the heart developing tooth germ, known as enamel knot. The primary knot forms in both incisors and molar germs cap stage development. Secondary tertiary knots only develop germs. These sit sites future tips from early bell In studies describing relationship between secondary knots, it is often assumed there cellular continuity these structures, such cells physically contribute to knots. We have...
Štembírek J, Buchtová M, Král T, Matalová E, Lozanoff S, Míšek I. Early morphogenesis of heterodont dentition in minipigs. Eur J Oral Sci 2010; 118: 547–558. © 2010 The minipig provides an excellent experimental model for tooth because its diphyodont and resemble that humans. However, little information is available on the processes development pig. purpose this study was to classify early stages odontogenesis minipigs from initiation deciduous late bell stage when successional dental lamina...
Caspases are well known proteases in the context of inflammation and apoptosis. Recently, novel roles pro-apoptotic caspases have been reported, including findings related to development hard tissues. To further investigate these emerging functions caspases, vivo localisation key (-3,-6,-7,-8, -9) was assessed, concentrating on two neighbouring tissues, cells participating odontogenesis (represented by first mouse molar) intramembranous osteogenesis (mandibular/alveolar bone). The expression...
Apoptosis during tooth development appears dependent on the apoptotic executioner caspase‐3, but not caspase‐7. Instead, activated caspase‐7 has been found in differentiated odontoblasts and ameloblasts, where it does correlate with apoptosis. To further investigate these findings, mouse incisor was used as a model. Analysis of caspase‐7‐deficient mice revealed significant thinner layer hard tissue adult incisor. Micro computed tomography scan confirmed this decrease mineralized tissues....
Caspase-9 is traditionally considered the initiator caspase of intrinsic apoptotic pathway. In past decade, however, other functions beyond initiation/execution cell death have been described including type-dependent regulation proliferation, differentiation/maturation, mitochondrial, and endosomal/lysosomal homeostasis. As previous studies revealed nonapoptotic caspases in osteogenesis bone homeostasis, this study was performed to identify proteins pathways deregulated by knockout caspase-9...