The first mouse molar (M1) is the most common model for odontogenesis, with research particularly focused on prenatal development. However, functional dentition forms postnatally, when histogenesis and morphogenesis of tooth completed, roots form physically anchors into jaw. In this work, M1 was studied from birth to eruption, assessing morphogenesis, proliferation apoptosis, correlating these remodeling surrounding bony tissue. completed crown formation between postnatal (P) days 0-2,...

Abstract Development of treatments for vocal dysphonia has been inhibited by lack human fold (VF) mucosa models because difficulty in procuring VF epithelial cells, cells’ limited proliferative capacity and absence cell lines. Here we report development engineered mucosae from hiPSC, transfected via TALEN constructs green fluorescent protein, that mimic cells utero. Modulation FGF signaling achieves stratified squamous epithelium definitive anterior foregut derived cultures. Robust culturing...

Chlastakova I, Lungova V, Wells K, Tucker AS, Radlanski RJ, Misek Matalova E. Morphogenesis and bone integration of the mouse mandibular third molar. Eur J Oral Sci 2011; 119: 265–274. © 2011 The molar (M3) develops postnatally is thus a unique model for studying non-mineralized tooth with mineralized bone. This study assessed morphogenesis M3, related to alveolar bone, comparing M3 development that first (M1), most common in odontogenesis. was evaluated from initiation eruption by...

ABSTRACT Congenital laryngeal webs result from failure of vocal fold separation during development in utero. Infants present with life-threatening respiratory problems at birth, and extensive lifelong difficulties breathing voicing. The molecular mechanisms that instruct formation are rarely studied. Here, we show, for the first time, conditional inactivation gene encoding β-catenin primitive laryngopharyngeal epithelium leads to folds, which approximates gross phenotype webbing. These...

The transcription factor c-Myb is involved in the control of cell proliferation, survival and differentiation. As these processes accompany morphogenesis developing teeth, this work investigates possible role during odontogenesis. Analysis expression monophyodont mouse was followed by similar analysis a diphyodont species, pig, which has dentition more closely resembling that human. distribution correlated with pattern proliferation apoptosis tooth phenotype mutant mice also assessed. In...

Vaping has been reported to cause acute epiglottitis, a life-threatening airway obstruction induced by direct epithelial injury and subsequent inflammatory reaction. Here, we show that were able recapitulate this phenomenon in vitro. Exposure of human engineered vocal fold (VF) mucosae 0.5% 5% electronic cigarette (e-cigarette) vapor extract (ECVE) for 1 week cellular damage luminal cells, disrupting homeostasis innate immune responses. Epithelial erosion was likely caused accumulation...

The MYB family of transcription activators has been associated with a high proliferation rate and an undifferentiated state cells in number tissues. Recently emerging data suggest that these molecules may also play role differentiation. In this study, the pattern expression c‐ was followed during postnatal stages mouse molar odontogenesis using immunohistochemistry on serial sections. Along abundance protein proliferating zones, we confirmed presence differentiated ameloblasts, odontoblasts,...

Structural changes to the vocal fold (VF) epithelium, namely, loosened intercellular junctions, have been reported in VF benign lesions. The potential mechanisms responsible for disruption of cell junctions do not address contribution resident microbial communities this pathological phenomenon. In study, we focused on determining relationship between Streptococcus pseudopneumoniae (SP), a dominant bacterial species associated with lesions, and salivarius (SS), commensal bacterium, human...

Mechanoreceptors are implicated as functional afferents within mucosa of the airways and recent discovery mechanosensitive channels Piezo1 Piezo2 has proved essential for cells various mechanically sensitive tissues. However, role Piezo1/2 in vocal fold (VF) mucosal epithelia, a cell that withstands excessive biomechanical insult, remains unknown. The purpose this study was to test hypothesis is required VF repair pathways epithelial injury. Utilizing sonic hedgehog (shh) Cre line...

Study of vocal fold (VF) mucosal biology requires essential human epithelial cell (hVFE) lines for use in appropriate model systems. We steadily transfected a retroviral construct containing telomerase reverse transcriptase (hTERT) into primary normal hVFE to establish continuously replicating line. Immortalized across passages have cobblestone morphology, express markers cytokeratin 4, 13 and 14, induced hTERT gene protein expression, similar RNAseq profiling, can grow more than 8 months....

The larynx, trachea, and esophagus share origin proximity during embryonic development. Clinical experimental evidence support the existence of neurophysiological, structural, functional interdependencies before birth. This investigation provides first comprehensive transcriptional profile all three organs organogenesis, where differential gene expression gradually assembles identity complexity these proximal from a shared in anterior foregut. By applying bulk RNA sequencing network analysis...

Current research approaches employ traditional tissue engineering strategies to promote vocal fold (VF) regeneration, whereas recent novel advances seek use principles of developmental biology guide generation by mimicking native cues, causing or allogenic/autologous progenitor cells undergo the regeneration process. To address paucity data direct VF differentiation and subsequent new formation, we characterize structure-proliferation relationships elastic moduli over embryonic development...

Abstract Electronic cigarettes (e-cigs) are nicotine delivery systems that have been touted as safer alternatives to smoking. A recently reported case of epiglottitis revealed a connection between vaping and swollen laryngeal vocal fold (VF) structures can lead acute life-threatening airway obstruction. The clinical course biopsy direct epithelial injury subsequent inflammatory reaction. Here we show were able recapitulate this phenomenon in vitro conditions. Exposure engineered VF mucosae...