A5004149455- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Neuroscience and Neural Engineering
- Osteoarthritis Treatment and Mechanisms
- Total Knee Arthroplasty Outcomes
- Nanofabrication and Lithography Techniques
- Analytical Chemistry and Sensors
- RNA Research and Splicing
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Electrochemical sensors and biosensors
- Musculoskeletal Disorders and Rehabilitation
- Electrospun Nanofibers in Biomedical Applications
- Nanoparticles: synthesis and applications
- Rheumatoid Arthritis Research and Therapies
- Bone Tissue Engineering Materials
- Advanced biosensing and bioanalysis techniques
- Cell Image Analysis Techniques
- Wound Healing and Treatments
- Biosensors and Analytical Detection
- Electrochemical Analysis and Applications
- Tissue Engineering and Regenerative Medicine
- Spectroscopy Techniques in Biomedical and Chemical Research
TU Wien
2016-2025
Medical University of Vienna
2020-2025
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
2022-2024
Ludwig Boltzmann Institute for Digital Health and Prevention
2022-2024
Austrian Cluster for Tissue Regeneration
2018-2022
University of Vienna
2022
Austrian Institute of Technology
2013-2015
The human placenta plays a crucial role as the interface between mother and fetus. It represents unique tissue that undergoes morphological well functional changes on cellular level throughout pregnancy. To better understand how works, variety of techniques has been developed to re-create this complex physiological barrier in vitro. However, due low availability freshly isolated primary cells, choriocarcinoma cell lines remain usual suspects vitro models for placental research. Here, we...
Versatile microfabrication of biocompatible OSTEMER using replica and microinjection molding enables fabrication complex multi-layered micropump 3D multi-membrane cell chips.
The placenta is a transient organ, essential for development and survival of the unborn fetus. It interfaces body pregnant woman with child secures transport endogenous exogenous substances. Maternal fetal blood are thereby separated at any time, by so-called placental barrier. Current in vitro approaches fail to model this multifaceted structure, therefore research field biology particularly challenging. present study aimed establishing novel model, simulating its implications on...
Abstract Physiological‐relevant in vitro tissue models with their promise of better predictability have the potential to improve drug screening outcomes preclinical studies. Despite advances spheroid pharmaceutical applications, variations size and consequential altered cell responses often lead nonreproducible unpredictable results. Here, a microfluidic multisize array is established characterized using liver, lung, colon, skin cells as well triple‐culture model blood‐brain barrier (BBB)...
Knowledge on the availability of dissolved oxygen inside microfluidic cell culture systems is vital for recreating physiological-relevant microenvironments and providing reliable reproducible measurement conditions. It important to highlight that in vivo cells experience a diverse range tensions depending resident tissue type, which can also be recreated vitro using specialized instruments regulate external concentrations. While cell-culture conditions readily adjusted state-of-the-art...
Abstract In the advent of affordable photo- and soft-lithography using polydimethylsiloxane (PDMS), low cost multi-step microfabrication methods have become available to a broad scientific community today. Although these are frequently applied for microfluidic prototype production in academic industrial settings, fast design iterations rapid prototyping within few minutes with high degree flexibility nearly impossible. To reduce concept-to-chip time costs, number alternative techniques...
Abstract The enhanced predictive power of 3D multi-cellular spheroids in comparison to conventional monolayer cultures makes them a promising drug screening tool. However, clinical translation for pharmacology and toxicology is lagging its technological progression. Even though show biological complexity resembling native tissue, standardization validation protocols are influenced by continuously changing physiological parameters during spheroid formation. Such cellular heterogeneities...
Abstract Dermal fibroblast cell migration is a key process in physiological wound healing. Therefore, the analysis of crucial for healing research. In this study, lab-on-a-chip technology was used to investigate effects basic growth factor (bFGF), mitomycin C (MMC), MEK1/2 inhibitor (U0126) and fetal calf serum (FCS) on human dermal migration. The microdevice fabricated consisting microchannels, pneumatic lines pneumatically-activated actuators by xurographic rapid prototyping. contrast...
We demonstrate that the integration of complex human synovial organ cultures in a lab-on-a-chip provides reproducible and reliable information on how systemic stress factors affect tissue architectures using light scatter biosensing.
Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to destruction of joint. The membrane as joint capsule's inner layer lined with fibroblast-like synoviocytes that are key player supporting persistent leading bone erosion and cartilage destruction. While microfluidic models model molecular aspects between bone-derived cells have been established, RA's synovial-chondral axis has not yet realised using 3D based on...
Luminescent chemical sensors have been proven to be a valuable asset in cell cultures and generally microbiological studies. We present microfluidic culture devices with integrated optical sensors. The offer the possibility monitor dissolved oxygen pH levels enable measurement of respiration acidification rates. metabolism was observed using this method by temporarily stopping incubation flow, barely influencing culture. A thermoplastic polymer is used as impermeable chip material rates,...
Abstract Lung cancer is a leading cause of death in both men and women worldwide. The high mortality rate lung part due to late-stage diagnostics as well spread cancer-cells organs tissues by metastasis. Automated detection its sub-types classification from cell’s images play crucial role toward an early-stage prognosis more individualized therapy. rapid development machine learning techniques, especially deep algorithms, has attracted much interest application medical image problems. In...
The assessment of drug-dose responses is vital for the prediction unwanted toxicological effects in modern medicine. Three-dimensional (3D) cell cultures techniques can provide vivo-like spheroids and microtissues that resemble natural tumor function. However, formation necrotic core diffusion limitation chemical compounds within these models reduce reproducibility precision standard bioassay protocols used to test two-dimensional (2D) cultures. Nonetheless, accurate detrimental based on...
Oxygen plays a pivotal role in cellular homeostasis, and its partial pressure determines function fate. Consequently, the ability to control oxygen tension is critical parameter for recreating physiologically relevant vitro culture conditions mammalian cells microorganisms. Despite importance, most microdevices organ-on-a-chip systems date overlook gradient parameters because controlling often requires bulky expensive external instrumental setups. To overcome this limitation, we have adapted...
Microfluidics cell-based assays require strong cell-substrate adhesion for cell viability, proliferation, and differentiation. The intrinsic properties of PDMS, a commonly used polymer in microfluidics systems, regarding interactions have limited its application assays. Various attempts by previous researchers, such as chemical modification, plasma-treatment, protein-coating PDMS revealed some improvements. These strategies are often reversible, time-consuming, short-lived with either...
A novel dopamine targeted electrochemical detection strategy has enabled the phenotyping and non-invasive monitoring of human midbrain organoids (healthy Parkinson's diseased), by employing a redox-cycling based microsensor.
A biomimetic stomach-on-a-chip with integrated peristalsis-like motion and intra-luminal flow. Application of dynamic conditions, elicited differentiation traits that are typical the normal gastric mucosa, but otherwise lost in 2D culture models.
Reengineering functional vascular networks in vitro remains an integral part tissue engineering, since the incorporation of non-perfused tissues results restricted nutrient supply and limited waste removal. Microfluidic devices are routinely used to mimic both physiological pathological microenvironments. Current procedures either involve investigation growth factor gradients interstitial flow on endothelial cell sprouting alone or heterotypic cell-cell interactions between mural cells....