A5051751389- Lymphatic System and Diseases
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Angiogenesis and VEGF in Cancer
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Planarian Biology and Electrostimulation
- Kruppel-like factors research
- Cellular Mechanics and Interactions
- Nonlinear Optical Materials Studies
- Tendon Structure and Treatment
- Cytokine Signaling Pathways and Interactions
- Photochromic and Fluorescence Chemistry
- NF-κB Signaling Pathways
- Rheumatoid Arthritis Research and Therapies
- Vascular Tumors and Angiosarcomas
- Electromagnetic Fields and Biological Effects
- Circular RNAs in diseases
- Cancer-related gene regulation
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Blood properties and coagulation
- Innovative Microfluidic and Catalytic Techniques Innovation
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
2016-2025
Ludwig Boltzmann Institute for Digital Health and Prevention
2021-2025
Austrian Cluster for Tissue Regeneration
2015-2024
University of Vienna
2021
Allgemeine Unfallversicherungsanstalt
2018-2020
BD Biosciences (United States)
2019
BioLegend (United States)
2019
University of Applied Sciences Technikum Wien
2012-2014
University of Helsinki
2008-2010
Institute for Molecular Medicine Finland
2010
Abstract Fumaric acid esters, mainly dimethylfumarate (DMF), have been successfully used to treat psoriasis. Based on previous observations that DMF inhibited expression of several TNF-induced genes in endothelial cells, we wished explore the molecular basis function greater detail. In first experiments analyzed effects tissue factor human cells culture, because is expressed by two independent sets transcription factors, NF-κB via TNF and early gene response-1 vascular growth (VEGF). We show...
The two-photon polymerization (2PP) of photosensitive gelatin in the presence living cells is reported. 2PP technique based on localized cross-linking photopolymers induced by femtosecond laser pulses. availability water-soluble photoinitiators (PI) suitable for crucial applying this method to cell-containing materials. Novel PIs developed our group allow formulations with up 80% cell culture medium. cytocompatibility these was evaluated an MTT assay. results encapsulation show occurrence...
The lack of efficient water-soluble two-photon absorption (TPA) photoinitiators has been a critical obstruction for three dimensional hydrogel microfabrications with high water load by induced polymerization (TPIP). In this paper, series cyclic benzylidene ketone-based initiators, containing carboxylic acid sodium salts to improve solubility, were synthesized via classical aldol condensation reactions. cytotoxicity cyclopentanone-based is as low that the well-known biocompatible...
The placenta is a transient organ, essential for development and survival of the unborn fetus. It interfaces body pregnant woman with child secures transport endogenous exogenous substances. Maternal fetal blood are thereby separated at any time, by so-called placental barrier. Current in vitro approaches fail to model this multifaceted structure, therefore research field biology particularly challenging. present study aimed establishing novel model, simulating its implications on...
In engineered heart tissues (EHT), oxygen and nutrient supply via mere diffusion is a likely factor limiting the thickness of cardiac muscle strands. Here, we report on novel method to in vitro perfuse EHT through tubular channels. Adapting our previously published protocols, expanded miniaturized fibrin-based EHT-format larger six-well format with six flexible silicone posts holding each (15×25×3 mm3). Thin dry alginate fibers (17×0.04×0.04 mm) were embedded into cell–fibrin–thrombin mix...
Abstract Tissue factor (TF) is the primary trigger of coagulation. Elevated levels TF are found in atherosclerotic plaques and leads to thrombus formation when released upon plaque rupture. Interleukin (IL)-33 was previously shown induce angiogenesis inflammatory activation endothelial cells (ECs). Here, we investigated impact IL-33 on human ECs, as a possible new link between inflammation induced mRNA protein umbilical vein ECs coronary artery ECs. IL-33-induced expression ST2-...
Abstract Low level light therapy receives increasing interest in the fields of tissue regeneration and wound healing. Several vivo studies demonstrated positive effects LLLT on angiogenesis. This study aimed to investigate underlying properties vitro by comparing emitting diodes different wavelengths endothelial cells . Human umbilical vein were treated with either 475 nm, 516 nm or 635 light. Control not illuminated. 2D proliferation was quantified manual counting. HUVEC migration analyzed...
Engineering three-dimensional (3D) hydrogels with well-defined architectures has become increasingly important for tissue engineering and basic research in biomaterials science. To fabricate 3D (sub)cellular-scale features, two-photon polymerization (2PP) shows great promise although the technique is limited by selection of appropriate hydrogel precursors. In this study, we report synthesis gelatin hydrolysate vinyl esters (GH-VE) its copolymerization reduced derivatives bovine serum albumin...
Vascularization of engineered tissues is one the current challenges in tissue engineering. Several strategies aim to generate a prevascularized scaffold which can be implanted at sites injury or trauma. Endothelial cells derived from peripheral blood (outgrowth endothelial cells, OECs) display promising features for vascular engineering, including their autologous nature, capacity proliferation and ability form mature vessels. In this study we investigated OECs structures co-culture with...
Vascular network engineering is essential for nutrient delivery to tissue-engineered constructs and, consequently, their survival. In addition, the functionality of tissues also depends on tissue drainage and immune cell accessibility, which are main functions lymphatic system. Engineering both blood microvasculature would advance survival constructs. The aim this study was isolate pure populations endothelial cells (LEC) vascular (BEC) from human dermal microvascular (HDMEC) formation in...
Knowledge on the availability of dissolved oxygen inside microfluidic cell culture systems is vital for recreating physiological-relevant microenvironments and providing reliable reproducible measurement conditions. It important to highlight that in vivo cells experience a diverse range tensions depending resident tissue type, which can also be recreated vitro using specialized instruments regulate external concentrations. While cell-culture conditions readily adjusted state-of-the-art...
Tissue-engineered constructs are promising to overcome shortage of organ donors and reconstruct at least parts injured or diseased tissues organs. However, oxygen nutrient supply limiting factors in many tissues, especially after implantation into the host. Therefore, development a vascular system prior appears crucial. To develop functional system, different cell types that interact with each other need be co-cultured simulate physiological environment vitro. This review provides an...
Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to destruction of joint. The membrane as joint capsule's inner layer lined with fibroblast-like synoviocytes that are key player supporting persistent leading bone erosion and cartilage destruction. While microfluidic models model molecular aspects between bone-derived cells have been established, RA's synovial-chondral axis has not yet realised using 3D based on...
Abstract Factors determining lymphatic differentiation in the adult organism are not yet well characterized. We have made observation that mixed primary cultures of dermal blood endothelial cells (BEC) and (LEC) grown under standard conditions change expression markers during subculture: After passage 6, they uniformly express LEC-specific Prox-1 podoplanin. Using sorted cells, we show LEC but BEC constitutively IL-3, which regulates podoplanin LEC. The addition IL-3 to medium induces...
A promising approach to overcome hypoxic conditions in tissue engineered constructs is use the potential of endothelial cells (EC) form networks vitro when co-cultured with a supporting cell type 3D environment. Adipose tissue-derived stromal (ASC) as well bone marrow-derived (BMSC) have been shown support vessel formation EC vitro, but only very few studies compared angiogenic both types using same model. Here, we aimed at investigating ability ASC and BMSC induce network co-culture model...