A5091827843- Bone Tissue Engineering Materials
- Silk-based biomaterials and applications
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Dental Implant Techniques and Outcomes
- Orthopaedic implants and arthroplasty
- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- Bone Metabolism and Diseases
- Bone fractures and treatments
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Estrogen and related hormone effects
- Bone and Dental Protein Studies
- Surfactants and Colloidal Systems
- Bone health and osteoporosis research
- Galectins and Cancer Biology
- Advanced Polymer Synthesis and Characterization
- Glycosylation and Glycoproteins Research
- Additive Manufacturing and 3D Printing Technologies
- Block Copolymer Self-Assembly
- Bone health and treatments
- Polymer crystallization and properties
- Cancer Cells and Metastasis
Eindhoven University of Technology
2016-2025
Springer Nature (Germany)
2021-2024
Swiss Academy of Sciences
2023
ETH Zurich
2010-2022
Institute for Biomedical Engineering
2010-2022
Klinik für Frauenheilkunde
2009-2020
Biomechanics Institute of Valencia
2014-2019
Ludwig-Maximilians-Universität München
2009-2019
Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe
2009-2019
École Polytechnique Fédérale de Lausanne
2005-2017
Fetal bovine serum (FBS) is a widely used supplement in cell culture medium, despite its known variability composition, which greatly affects cellular function and consequently the outcome of studies. In bone tissue engineering, deposited mineralized matrix one main parameters, but using different brands FBS can result large variations. Alkaline phosphatase (ALP) present FBS. Not only ALP to judge osteogenic differentiation cells, it may affect deposition matrix. The study focused on...
Porous biodegradable silk scaffolds and human bone marrow derived mesenchymal stem cells (hMSCs) were used to engineer bone-like tissue in vitro. Two different with the same microstructure studied: collagen (to assess effects of fast degradation) covalently bound RGD sequences enhanced cell attachment slow degradation). The hMSCs isolated, expanded culture, characterized respect expression surface markers ability for chondrogenic osteogenic differentiation, seeded on scaffolds, cultured up 4...
Abstract Bone morphogenetic protein (BMP)‐2 has a critical role in bone formation and regeneration. Therefore, the ability to immobilize this molecule certain matrices may be crucial tissue engineering. Using carbodiimide chemistry, BMP‐2 was directly immobilized on silk fibroin films. Whereas human marrow stromal cells cultured unmodified films presence of osteogenic stimulants exhibited little if any osteogenesis, same decorated differentiated into an osteoblastic lineage as assessed by...
Abstract Human mesenchymal stem cells (hMSC) derived from bone marrow aspirates can form the basis for in vitro cultivation of autologous tissue grafts and help alleviate problems immunorejection disease transmission associated with use allografts. We explored utility hMSC cultured on protein scaffolds engineering cartilage. were isolated, expanded culture, characterized respect to expression surface markers ability chondrogenic osteogenic differentiation, seeded scaffolds. Four different...
Current tissue engineering techniques have various drawbacks: they often incorporate uncontrolled and imprecise scaffold geometries, whereas the current conventional cell seeding result mostly in random placement rather than uniform distribution. For successful reconstruction of deficient tissue, new material approaches to be considered overcome limitations. An emerging method produce complex biological products including cells or extracellular matrices a controlled manner is process called...
Silk fibroin scaffolds were studied as a new biomaterial option for tissue-engineered cartilage-like tissue. Human bone marrow–derived mesenchymal stem cells (MSCs) seeded on silk, collagen, and crosslinked collagen cultured 21 days in serum-free chondrogenic medium. Cells proliferated more rapidly the silk than matrices. The total content of glycosaminoglycan deposition was three times higher compared to scaffolds. Glycosaminoglycan coincided with overexpression type II aggrecan genes....
New regenerative materials and approaches need to be assessed through reliable comparable methods for rapid translation the clinic.There is a considerable proven in vitro assays that are able reduce burden on animal testing, by allowing assessment of biomaterial utility predictive results currently obtained vivo studies.The purpose this multicentre review was investigate correlation between existing with outcomes observed range biomaterials.Members from European consortium BioDesign,...
The orientation of seven iridium-based emitter molecules for OLEDs is compared and surprisingly all except <bold>Ir(ppy)3</bold> show considerable horizontal orientation.
Injectable scaffolds are of interest in the field regenerative medicine because their minimally invasive mode delivery. For tissue repair applications, it is essential that such have mechanical properties, porosity and pore diameter to support formation new tissue. In current study, porous poly(dl-lactic acid-co-glycolic acid) (PLGA) microspheres were fabricated with an average size 84±24μm for use as injectable cell carriers. Treatment ethanolic sodium hydroxide 2min was observed increase...
Abstract Bone formation (osteogenesis) is a complex process in which cellular differentiation and the generation of mineralized organic matrix are synchronized to produce hybrid hierarchical architecture. To study mechanisms osteogenesis health disease, there great need for functional model systems that capture parallel, both processes. Stem cell‐based organoids promising as functional, self‐organizing 3D vitro models studying physiology pathology various tissues. However, human bone, no...
Silk fibroin is an important polymer for scaffold designs, forming biocompatible and mechanically robust biomaterials bone, cartilage, ligament tissue engineering. In the present work, 3D biomaterial matrices were fabricated from silk with controlled pore diameter interconnectivity, utilized to engineer bone starting human mesenchymal stem cells (hMSC). Osteogenic differentiation of hMSC seeded on these scaffolds resulted in extensive mineralization, alkaline phosphatase activity, formation...
Abstract To obtain sufficient cell numbers for cartilage tissue engineering with autologous chondrocytes, cells are typically expanded in monolayer culture. As a result, they lose their chondrogenic phenotype process called dedifferentiation, which can be reversed upon transfer into 3D environment. We hypothesize that the properties of this environment, namely adhesion site density and substrate elasticity, would influence redifferentiation process. test hypothesis, chondrocytes were...