A5083739043- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Orthopaedic implants and arthroplasty
- Connective tissue disorders research
- Tissue Engineering and Regenerative Medicine
- Dental Implant Techniques and Outcomes
- 3D Printing in Biomedical Research
- Spine and Intervertebral Disc Pathology
- Bone fractures and treatments
- Metal and Thin Film Mechanics
- Surgical Sutures and Adhesives
- Bone health and osteoporosis research
- Spinal Fractures and Fixation Techniques
- Proteoglycans and glycosaminoglycans research
- Electrospun Nanofibers in Biomedical Applications
- Advanced materials and composites
- Neonatal Respiratory Health Research
- Hemostasis and retained surgical items
- Mesenchymal stem cell research
- TGF-β signaling in diseases
- Hydrogels: synthesis, properties, applications
- Polymer Surface Interaction Studies
- Congenital heart defects research
- Monoclonal and Polyclonal Antibodies Research
- Bone Metabolism and Diseases
Uppsala University
2014-2025
Science for Life Laboratory
2021-2024
University of Southampton
2016-2018
Southampton General Hospital
2016
Uppsala University Hospital
2013
New regenerative materials and approaches need to be assessed through reliable comparable methods for rapid translation the clinic.There is a considerable proven in vitro assays that are able reduce burden on animal testing, by allowing assessment of biomaterial utility predictive results currently obtained vivo studies.The purpose this multicentre review was investigate correlation between existing with outcomes observed range biomaterials.Members from European consortium BioDesign,...
Abstract Biomaterial development for tissue engineering applications is rapidly increasing but necessitates efficacy and safety testing prior to clinical application. Current in vitro vivo models hold a number of limitations, including expense, lack correlation between animal human outcomes the need perform invasive procedures on animals; hence requiring new predictive screening methods. In present study we tested hypothesis that chick embryo chorioallantoic membrane (CAM) can be used as...
Regeneration of bone by delivery morphogenetic proteins (BMPs) from implantable scaffolds is a promising alternative to the existing autologous grafting procedures. Hydrogels are used extensively in biomaterials as systems for different growth factors. However, controlled release factors necessary induce formation, which can be accomplished various chemical functionalities. Herein we demonstrate that functionalization hyaluronan (HA) hydrogel with covalently linked bisphosphonate (BP)...
Background and Purpose: Cerebral cavernous malformations (CCM) present as mulberry-like of the microvasculature central nervous system. Current medical treatment CCM lesions is limited to surgical removal vascular malformations. It is, therefore, important identify therapeutic drug treatments for patients with CCM. Propranolol has shown great benefit in infantile hemangioma. In addition, who receive propranolol have demonstrated a reduction their lesions. Our investigation set out provide...
The development of biomaterial for bone regeneration requires animal models that are reliable and designed to mimic clinically relevant situations. We have previously investigated hydrogels comprised modified hyaluronic acid polyvinyl alcohol in ectopic formation. This hydrogel induces when loaded with morphogenetic proteins (BMPs). To allow further optimization hydrogels, we developed a new, femoral, non-critical-sized cortical defect model. In the rat femur, drilled standardized, elongated...
Despite technological advancements, further innovations in the field of orthopaedics and bone regeneration are essential to meet rising demands an increasing aging population associated issues disease, injury trauma. Nanotopography provides new opportunities for novel implant surface modifications promises deliver improvements performance. However, technical complexities nanotopography fabrication analysis have precluded identification optimal features trigger osteogenesis. We herein detail...
In this work a radiopaque premixed calcium phosphate cement (pCPC) has been developed and evaluated in vivo . Radiopacity was obtained by adding 0–40 % zirconia to the paste. The effects of on setting time, strength radiopacity were evaluated. study 2 3.5 mm cylindrical defect rat vertebrae filled with either pCPC, PMMA or bone chips. Nano-SPECT CT analysis used monitor osteoblast activity during regeneration. showed that time becomes longer compressive is reduced. All materials there strong...
Abstract Treatment of osteoporotic fractures with conventional surgical methods is associated a high rate complications. Intense search for new treatment options includes development specific biomaterials aimed to be part the armamentarium. Strontium doped calcium phosphate spheres (SrCPS) material that might interest due influence on osteoclast and osteoblast activity. In present study, we successfully constructed hollow spherical SrCPS particles diameter ∼700 nm shell thickness ∼150 nm....
Currently there are no standard models with which to evaluate the biomechanical performance of calcified tissue adhesives, in vivo. We present, herein, a pre-clinical murine distal femoral bone model for evaluating adhesives intended use both osseous and osteochondral reconstruction. Cylindrical cores (diameter (Ø) 2 mm (mm) × depth), containing cancellous cortical bone, were fractured out from femur then reattached using one two adhesives. The adhesiveness fibrin glue (Tisseeltm), novel,...
Abstract A new class of materials, bone adhesives, could revolutionise the treatment highly fragmented fractures. We present first biological safety investigation a bio-inspired adhesive. The formulation was based upon modified calcium phosphate cement that included amino acid phosphoserine. This material has recently been described as substantially stronger than other bioresorbable cements. Four adhesive groups with active substance (phosphoserine) and two control without phosphoserine were...
Traditionally used as an angiogenic assay, the chorioallantoic membrane (CAM) assay of chick embryo offers significant potential in vivo model for xenograft organ culture. Viable human bone can be cultivated on CAM and increases volume are evident; however, it remains unclear by what mechanism this change occurs whether reflects physiological process remodelling. In study we tested hypothesis that CAM-induced remodelling is a consequence host graft mediated processes. Bone cylinders...
Osteoporotic fractures are a growing issue due to the increasing incidence of osteoporosis worldwide. High reoperation rates in osteoporotic call for investigation into new methods improving fixation bones. In present study, strength recently developed bone bioadhesive, OsStictm, was evaluated vivo using novel core assay murine animal model at 0, 3, 7, 14, 28, and 42 days. Histology micro-CT were obtained all time points, mean peak pull-out force assessed on days 0-28. The adhesive provided...
In the characterization of severe lung diseases, early detection specific inflammatory cells could help to monitor patients' response therapy and increase chances survival. Macrophages contribute regulating resolution termination inflammation have increasingly been interest for targeted therapies. [68Ga]Ga-DOTA-TATE is an established clinical radiopharmaceutical targeting somatostatin receptor subtype 2 (SSTR 2). Since activated macrophages (M1) overexpress SSTR 2, aim this study was...
Bone morphogenetic proteins (BMP's) are vital for bone and cartilage formation, where protein-2 (BMP-2) is acknowledged as a growth factor in osteoblast differentiation. However, uncontrolled delivery may result adverse clinical effects. In this study we investigated the possibility longitudinal non-invasive monitoring of implanted [125I]BMP-2 retention its relation to ossification at site implantation. A unilateral critically sized femoral defect was produced left limb rats while right...
This study presents a thorough in vitro and vivo characterization of the delivery bone morphogenetic protein 2 (BMP-2) from hyaluronan-based hydrogel system. The release BMP-2 similar hydrogels has previously been studied by enzyme-linked immunosorbent assay (ELISA), which only fraction loaded is detected. In current study, 125I radiolabelling was used instead to monitor vivo. To minimize loss during handling, 125I-BMP-2 adsorption different tubes at times temperatures. data showed that...